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Profile and development of microsatellite primers for acanthogobius ommaturus based on high-throughput sequencing technology

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Journal of Oceanology and Limnology 2020年第6期38卷 1880-1890页

作者： SONG Chenyu FENG Ziyi LI Chunhou SUN Zhicheng GAO Tianxiang SONG Na LIU Lu Fisheries College Ocean University of ChinaQingdao 266003China South China Sea Fisheries Research Institute Chinese Academy of Fishery SciencesGuangzhou 510300China National Engineering Research Center for Marine Aquaculture Zhejiang Ocean UniversityZhoushan 316022China

acanthogobius ommaturus,a fish species of the Family Gobiidae,is a marine commercial fish perched on the bottom of *** this study,Illumina high-throughput sequencing technology was applied to obtain the candidate microsatellite markers of ***.A total of 4746 microsatellite-rich fragments were found,of which 4542 microsatellites are with primer fragments,containing 971 dinucleotide sequences,2643 trinucleotide sequences,569 tetranucleotide sequences,406 pentanucleotide sequences,and 212 hexanucleotide *** on the results of high-throughput sequencing,a total of 141 pairs of the microsatellite primers were designed and *** then 24 polymorphic primers were finally obtained by polyacrylamide gel *** total,271 alleles were detected in the 24 pairs of *** number of alleles for different primers ranged from 5 to *** average number of effective alleles(Na)was 11.292;the average observed heterozygosity(Ho)of the 24 pairs of primers was 0.665,the average expected heterozygosity(He)was 0.880,and the average polymorphic information content was *** sites were highly polymorphic(PIC>0.50).

关键词： microsatellite acanthogobius ommaturus high-throughput sequencing polymorphic sites

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Discordant patterns of genetic variation between mitochondrial and microsatellite markers in acanthogobius ommaturus across the coastal areas of China

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Acta Oceanologica Sinica 2023年第4期42卷 72-80页

作者： Chenyu Song Zhong Tu Na Song Key Laboratory of Mariculture(Ocean University of China) Ministry of EducationQingdao 266003China Shandong Fisheries Development and Resources Conservation Center Yantai 264003China

acanthogobius ommaturus, which belongs to the family Gobiidae, is a euryhaline and demersal fish that is widely distributed in the coastal areas, harbors, and estuaries of China, D. P. R. Korea and Japan. In this study, the genetic diversity and genetic structure of five geographical populations of A. ommaturus was assessed using the mitochondrial hypervariable region gene and microsatellite markers. The results of the two genetic markers indicated that the A. ommaturus populations had a high level of genetic diversity. The mitochondrial marker detected weak genetic differentiation among populations, and the Neighbor-Joining tree showed that there was no obvious pedigree branches and geographic structure as well. However, population of Zhoushan showed significant genetic differentiation with other populations by microsatellite markers. The population of *** has not experienced bottleneck effect recently. We speculated that the Pleistocene climate change and juvenile fish dispersal played an important role in the population differentiation of A. ommaturus.

关键词： mitochondrial markers microsatellite markers genetic diversity genetic structure acanthogobius ommaturus

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