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普鲁卡因对离体人红细胞膜atpases活性的影响

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中华麻醉学杂志 1994年第3期14卷 189-191页

作者：尹宁林桂芳刘存明钱燕宁镇江市第一人民医院麻醉科邮政编码212002 南京医学院第一附属医院

盐酸普鲁卡因是静脉复合麻醉（ＩＢＡ）的常用药物之一。我们发现在ＩＰＢＡ下施行上腹部手术时，术毕人红细胞膜ＡＴＰａｓｅｓ活性有所下降。本研究的目的是观察普鲁卡因对离体人红细胞膜ＡＴ－Ｐａｓｅ活性是否有直接影响。结果；... 详细信息

盐酸普鲁卡因是静脉复合麻醉（ＩＢＡ）的常用药物之一。我们发现在ＩＰＢＡ下施行上腹部手术时，术毕人红细胞膜ＡＴＰａｓｅｓ活性有所下降。本研究的目的是观察普鲁卡因对离体人红细胞膜ＡＴ－Ｐａｓｅ活性是否有直接影响。结果；一定浓度的普鲁卡因对Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ活性有明显抑制作用，对Ｍｇ２＋－ＡＴＰａｓｅ活性无明显作用，对Ｃａ２＋－Ｍｇ２＋－ＡＴＰａｓｅ活性有轻微保护作用。研究结果为临床合理应用ＩＰＢＡ提供了一定的实验依据。

关键词：麻醉普鲁卡因红细胞膜 atpases

芬太尼对离体红细胞膜atpases活性的影响

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中华麻醉学杂志 1996年第6期16卷 253-254页

作者：尹宁林桂芳钱燕宁南京医科大学附属第一医院

选用静脉普鲁卡因复合麻醉(IPBA)中伍用镇痛药芬太尼,按一定浓度作用于离体状态人红细胞膜,结果发现:当芬太尼浓度>10μg/L对人红细胞膜Na^+-K^+-ATPase活性开始出现轻微抑制;浓度>50μg/L有明显抑制作用(P<0.01)。任何浓度芬太尼对Mg^(... 详细信息

选用静脉普鲁卡因复合麻醉(IPBA)中伍用镇痛药芬太尼,按一定浓度作用于离体状态人红细胞膜,结果发现:当芬太尼浓度>10μg/L对人红细胞膜Na^+-K^+-ATPase活性开始出现轻微抑制;浓度>50μg/L有明显抑制作用(P<0.01)。任何浓度芬太尼对Mg^(2+)-ATPase无明显影响,对Ca^(2+)-Mg^(2+)-ATPase有轻度抑制,抑制程度不随芬太尼浓度的增减而显著改变。

关键词：麻醉芬太尼红细胞膜 atpases

平邑甜茶根系质膜atpases活性的调控及其与根系营养吸收的关系研究

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平邑甜茶根系质膜ATPases活性的调控及其与根系营养吸收的关系研究

作者：马方放山东农业大学

学位级别：硕士

本试验以具有无融合生殖能力的平邑甜茶(Malus hupehensis(Pamp)Rehd.)当年实生苗或2-3年生幼树为试材,在水培、砂培和盆栽条件下,研究了四种营养物质、四种植物生长物质、根系修剪和秋施基肥对根系质膜atpases、蛋白激酶活性和根系营... 详细信息

本试验以具有无融合生殖能力的平邑甜茶(Malus hupehensis(Pamp)Rehd.)当年实生苗或2-3年生幼树为试材,在水培、砂培和盆栽条件下,研究了四种营养物质、四种植物生长物质、根系修剪和秋施基肥对根系质膜atpases、蛋白激酶活性和根系营养吸收速率的调节,并结合抑制剂试验和回归分析探讨了atpases活性与根系营养吸收速率间的关系,结果如下:1. NaH2PO4、KCl、Ca(NO3)2和MgCl2均可显著激活根系质膜H+-ATPase和Ca2+-ATPase的活性;NaH2PO4、KCl、Ca(NO3)2可提高质膜H+-ATPase的PNPP水解活性,降低H+-ATPase和PNPP间的Km值。2. NaH2PO4、KCl和MgCl2可提高质膜蛋白总激酶的活性,且激酶活性与质膜atpases酶活的变化间有显著的相关性。3. NaH2PO4处理下根系吸收Fe,Ca,Mn,Zn,Cu的速率变化和质膜atpases活性呈显著相关,与同样处理下质膜总蛋白激酶活性的变化趋势相似但无相关性。4.白色根和褐色根质膜atpases活性对生长物质反应不同,吲哚乙酸(IAA,10-80μg/g)和萘乙酸(NAA,10-40μg/g)对白色根质膜H+-ATPase和Ca2+-ATPase的激活程度均强于褐色木质根,而高浓度的IAA(100μg/g)、NAA(50μg/g)对褐根质膜atpases活性的抑制程度高于白色根。低浓度的茉莉酸甲酯(5μmol/L,MeJA)和壳羧孢菌素(2.5μg/g,FC)可显著激活质膜atpases的活性。IAA可提高白色根和褐色根质膜H+-ATPase PNPP的水解活性,降低H+-ATPase和PNPP间的Km值;而MeJA和FC对质膜H+-ATPase的PNPP水解活性无影响。5. IAA, MeJA和FC能够激活质膜蛋白总激酶的活性,且与atpases活性变化间有显著的相关性。

关键词：平邑甜茶根系质膜 atpases 蛋白激酶营养吸收

咪唑安定对离体人红细胞膜atpases及脂质流动性的影响

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海南医学 2003年第12期14卷 53-54页

作者：张斯璧武欣生周力陈珏钱燕宁南京市儿童医院南京210008 江苏省人民医院南京210029

目的　探讨咪唑安定对离体人红细胞膜atpases及脂质流动性的影响。方法　将不同浓度咪唑安定 (A组 0浓度 ,B组 4ng/ml,C组 40ng/ml,D组 40 0ng/ml,E组 4μg/ml,F组 40 μg/m)与离体人红细胞孵育后 ,提取红细胞膜 ,测各组红细胞膜ATPase... 详细信息

目的　探讨咪唑安定对离体人红细胞膜atpases及脂质流动性的影响。方法　将不同浓度咪唑安定 (A组 0浓度 ,B组 4ng/ml,C组 40ng/ml,D组 40 0ng/ml,E组 4μg/ml,F组 40 μg/m)与离体人红细胞孵育后 ,提取红细胞膜 ,测各组红细胞膜atpases及脂质流动性 ,并进行比较。结果　采用双因素方差分析 ,各组间atpases活性无显著差异 ,F组膜脂质流动性显著下降 (P atpases及脂质流动性影响无显著影响 ,从而推断咪唑安定在临床使用中安全范围较广。

关键词：咪唑安定红细胞膜 atpases 脂质流动性低渗溶血法血药浓度

Potassium Bromate-induced Changes in the Adult Mouse Cerebellum Are Ameliorated by Vanillin

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Biomedical and Environmental Sciences 2018年第2期31卷 115-125页

作者： Hajer Ben Saad Dorra Driss Imen Jaballi Hanen Ghozzi Ons Boudawara Michael Droguet Christian Magne Monsef Nasri Khaled Mounir Zeghal Ahmed Hakim Ibtissem Ben Amara Laboratory of Pharmacology University of SfaxFaculty of Medicine3029 SfaxTunisia Laboratory of Enzyme engineering and Microbiology National Engineering School in SfaxUniversity of SfaxB.P.11733038 SfaxTunisia Centre of Biotechnology of Sfax P.O.Box 11773018 SfaxTunisia Anatomopathology Laboratory University of SfaxHabib Bourguiba Hospital3029 SfaxTunisia EA 4324 ORPHY Faculty of MedicineUniversity of Brest29238 Brest CedexFrance EA 2219 Geoarchitecture University of BrestUFR Sciences&Techniques29238 Brest Cedex 3France

Objective The current study aimed to elucidate the effect of vanillin on behavioral changes, oxidative stress, and histopathological changes induced by potassium bromate （KBrO3）, an environmental pollutant, in the cerebellum of adult *** The animals were divided into four groups： group 1 served as a control, group 2 received KBrO3, group 3 received KBrO3 and vanillin, and group 4 received only vanillin. We then measured behavioral changes, oxidative stress, and molecular and histological changes in the *** We observed significant behavioral changes in KBrO3-exposed mice. When investigating redox homeostasis in the cerebellum, we found that mice treated with KBrO3 had increased lipid peroxidation and protein oxidation in the cerebellum. These effects were accompanied by decreased Na＋-K＋ and Mg2＋ ATPase activity and antioxidant enzyme gene expression when compared to the control group. Additionally, there was a significant increase in cytokine gene expression in KBrO3-treated mice. Microscopy revealed that KBrO3 intoxication resulted in numerous degenerative changes in the cerebellum that were substantially ameliorated by vanillin supplementation. Co-administration of vanillin blocked the biochemical and molecular anomalies induced by *** Our results demonstrate that vanillin is a potential therapeutic agent for oxidative stress associated with neurodegenerative diseases.

关键词： Cerebellum Behavior Potassium bromate Vanillin atpases Genes expression

Endogenous modulators in the regulation of ion transporting enzymes: structure, function, interactions, recent advancements and future perspectives

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Advances in Biological Chemistry 2011年第3期1卷 74-92页

作者： Parimal C. Sen 不详

A prerequisite for life is the ability to uphold electro-chemical imbalance across biomembranes. Ion trans- porting enzymes, known as specific pumps, are re-sponsible for the transport of various ions across cell membranes to sustain the same. In all eukaryotes, the plasma membrane potential and secondary transport systems are maintained by the activity of P-type ion transporting enzymes, commonly known as ATPase membrane pumps. Malfunction of pumps leads to various cell disorders and subsequently diseases like cardiac problems, renal malfunctionings, diabetes, cataract, even cancer. Activities/functions of these pumps are regulated either by exogenous agents or by endogenous substances like proteins, peptides, hormones, etc., which are collectively known as mo- dulators. Some of these endogenous modulators may be useful for developing drugs depending on the na-ture of regulation. For more than last two decades, researchers across the globe are exploring the me- chanism of action of different endogenous modulators on these ion transporting enzymes with the aim of developing target-specific drugs. In this review, we have discussed recent advances in our understanding of ATPase pumps, e.g., Ca2+-, Na+, K+-, Ca2+, Mg2+-, H+, K+-atpases, with the emphasis on their functional regulation by a number of endogenous modulators, and the implications of development of some of these modulators as potential drugs.

关键词： atpases Endogenous Protein/Peptides FX- YD Phospholamban Sarcolipin Interleukin Regulations.

Changes in erythrocyte membrane ATPase and plasma lipid peroxides during perioperative period

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医学研究生学报 1993年第S1期6卷 50-54页

作者： Tu Weifeng Lin Guifang Shen Jianfan Department of Anesthesiology,Jinling Hospital,Nanjing,China Department of Anesthesiology,First Affiliated Hospital of Nanjing Medical College,Nanjing,China

Changes in erythrocyte membrane atpases and plasma lipid peroxides（LPO） were studiedperioperatively in 15 abdominal surgical patients under intravenous procaine-balanced *** resultsrevealed that erythrocyte membrne Na+-K+ATPase,Mg2+ATPase and Ca2+-Mg2+ATPase activities werenot significantly changed at 60～90 min after incision as compared with 30 min before anesthesia,but weredecreased markedly at 10 rain and 24 hours after completion of operation（P0.05）.These correlations are suggested to be inducedby neuroendoerine responses after anesthesia and/or surgical trauma.

关键词： erythrocytes membrane atpases lipid peroxides balanced anesthesia