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Inhibiting NF-κB increases cholesterol efflux from THP-1 derived-foam cells treated with AngⅡ via up-regulating the expression of atp-binding cassette transporter a1

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Journal of Nanjing Medical University 2008年第4期22卷 211-216页

作者： Kun Liu Yanfu Wang Zhijian Chen Yuhua Liao Xiang Gao Jian Chen Hua Zhong University of Science and Technology Union Hospital Institute of Cardiology Wuhan430022 Hubei Province China

Objective： To study the role of nuclear factor-kappa B（NF- κB） in cholesterol efflux from THP-1 derived-foam cells treated with Angiotensin Ⅱ（Ang Ⅱ ）. Methods：Cultured THP-1 derived-foam cells were treated with Ang Ⅱ or preincubated with tosyl-phenylalanine chloromethyl-ketone（TPCK） NF- κB inhibitor. The levels of activated NF- κB in the cells were examined by sandwich ELISA, Cellular cholesterol content was studied by electron microscopy scanning and zymochemistry via fluorospectrophotometer and cholesterol effiux was detected by scintillation counting technique. ABCa1 mRNA and protein were quantified by RT-PCR and Western blotting. Results：Addition of TPCK to the cells before Ang Ⅱ stimulation attenuated the response of NF- κB p65 nuclear translocation induced by Ang Ⅱ and showed no peak in foam cells group and caused a reduction in cholesterol content and an increase in cholesterol efflux by 24.1%（P〈 0.05） and 41.1%（P〈 0.05） respectively, when compared with Ang Ⅱ group. In accordance, the ABCa1 mRNA and protein were increased by 30% and 19%（P 〈 0.05） respectively, when compared with Ang Ⅱ group. Conclusion：Ang Ⅱ can downregulate ABCa1 in THP-1 derived-foam cells via NF- K B, which leads to less cholesterol effiux and the increase of cholesterol content with the consequence of the promotion of atherosclerosis.

关键词： Angiotensin Ⅱ nuclear factor- kappa B atp-binding cassette transporter a1 cholesterol effiux atherosclerosis

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Protective Effect of Irbesartan on atp binding cassette transporter a1 in THP-1 Derived Macrophages

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South China Journal of Cardiology 2009年第4期10卷 227-233页

作者：张慧玲李清贤王彦富石胜伟 The Affiliated Hospital of Jining Medical College

Objectives To explore the protective effect of irbesartan （lrb） under the interference with angiotensin II （Ang II） on ABCa1. Methods Electron microscopy was used to detect the morphous of foam cells. The expression of ABCa1 mRNA and its protein were determined by RT-PCR and Western blotting, respectively. The variance of cellular cholesterol content was measured by zymochemistry via-fluorospeetrophotometer. Results A positive facilitative effect of Ang II on the formation of foam cells was observed. Total cholesterol was increased significantly by Ang II, the expression of ABCa1 was down-regulated obviously by Ang II; Irb could protect ABCa1 against the lesion of Ang II; Total cellular cholesterol content was reduced significantly in Irb ＋ Ang II group; However, considerable alteration about the cholesterol content and the expression of ABCa1 were not observed in Irb group without incubation with Ang II. Conclusions Irb could protect ABCa1 against the lesion of Ang II, which may contribute to its anti-atherosclerotic properties. （ S Chin J Cardiol 2009; 10（4）： 227 -233）

关键词： atherosclerosis angiotensin II irbesartan atp-binding cassette transporter a1

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A Possible Mechanism Linking Hyperglycemia and Reduced High-density Lipoprotein Cholesterol Levels in Diabetes

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2010年第3期30卷 318-321页

作者：高峰严同赵艳尹凡胡翠宁 Department of Endocrinology Union HospitalTongji Medical CollegeHuazhong University of Science and Technology

This study investigated the role of glucose in the biogenesis of high-density lipoprotein cholesterol(HDL-C).Mouse primary peritoneal macrophages were harvested and maintained in Dulbecco’s modified Eagle’s medium(DMEM) containing glucose of various *** cells were divided into 3 groups in terms of different glucose concentrations in the cultures:Control group(5.6 mmol/L glucose),high glucose concentration groups(16.7 mmol/L and 30 mmol/L glucose).atp-binding cassette transporter a1(ABCa1) mRNA expression in the macrophages was detected by semi-quantitative RT-PCR 24,48 and 72 h after glucose *** results showed that ABCa1 mRNA expression in the 16.7 mmol/L glucose group was not significantly different from that in the control group at all testing time points(P>0.05 for each).In the 30 mmol/L glucose group,macrophage ABCa1 mRNA expression was not changed significantly at 24 h(P=0.14),but was substantially decreased by 40.4% at 48 h(P=0.009) and by 48.1% at 72 h(P=0.015) as compared with that in the control *** was concluded that ABCa1 is of vital importance for HDL-C *** glucose may hamper HDL-C biogenesis by decreasing ABCa1 expression,which contributes to low HDL-C level in diabetes.

关键词： reverse cholesterol transport diabetes high-density lipoprotein cholesterol atp-binding cassette transporter a1

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Two pyrrole acids isolated from Phyllanthus emblica *** their bioactivities

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Natural Products and Bioprospecting 2023年第1期13卷 493-501页

作者： Shu-Hui Wang Cong Guo Wen-Jin Cui Qing-Xia Xu Jun Zhang Jin-Zhu Jiang Yan Liu Sha Chen Chang Chen Jin-Tang Cheng An Liu Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing 100700China

An undescribed pyrrole acid,1-(4′-methoxy-4′-oxobutyl)-1 H-pyrrole-2,5-dicarboxylic acid(1)and one known pyr-role acid(2)were isolated from the fruits of Phyllanthus *** structures of these compounds were elucidated via the comprehensive analyses of IR,HRESIMS,1D and 2D spectroscopic data.A series of biological assays revealed that compounds 1 and 2 could inhibit LPS-induced over-production of nitric oxide(NO),interleukin-6(IL-6),monocyte chemotactic protein 1(MCP-1)and tumor necrosis factor-α(TNF-α)by reducing the phosphorylation of extracellular regulated protein kinases(ERK)and c-Jun N-terminal kinases(JNK)in RAW 264.7 ***,compounds 1 and 2 were found to reduce lipid deposition and increase the mRNA expression of atp-binding cassette transporter a1 in oxidized low-density lipoprotein-treated RAW264.7 macrophages.

关键词： Pyrrole acid Phyllanthus emblica L. Lipid deposition atp-binding cassette transporter a1 RAW264.7 macrophages

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