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New Insights into Aluminum Tolerance in Rice： The asr. Protein Binds the STAR1 Promoter and Other Aluminum-Responsive Genes

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Molecular Plant 2014年第4期7卷 709-721页

作者： Rafael Augusto Arenhart Yang Bai Luiz Felipe Valter de Oliveira Lauro Bucker Neto Mariana Schunemann Felipe dos Santos Maraschin Jorge Mariath Adriano Silverio Gilberto Sachetto-Martins Rogerio Margis Zhi-Yong Wang Marcia Margis-Pinheiro Programa de Pos-Graduacao em Genetica e Biologia Molecular Avenida Bento Goncalves 9500 Departamento de Genetica sala 207 predio 43312 Universidade Federal do Rio Grande do Sul 91501-970 Porto Alegre Brasil Department of Plant Biology Carnegie Institution for Science Stanford CA 94305 USA School of Computer Science and Technology Harbin Institute of Technology Harbin 150001 China Departamento de Botanica Universidade Federal do Rio Grande do Sul Porto Alegre Brasil Departamento de Genetica Universidade Federal do Rio de Janeiro Porto Alegre Brasil Centro de Biotecnologia Universidade Federal do Rio Grande do Sul Porto Alegre Brasil

Aluminum （AI） toxicity in plants is one of the primary constraints in crop production. Al3＋, the most toxic form of Al, is released into soil under acidic conditions and causes extensive damage to plants, especially in the roots. In rice, Al tolerance requires the asr. gene, but the molecular function of asr. has remained unknown. Here, we perform genome-wide analyses to identify asr.-dependent Al-responsive genes in rice. Based on asr._RNAi silencing in plants, a global transcriptome analysis identified a total of 961 genes that were responsive to Al treatment in wildtype rice roots. Of these genes, 909 did not respond to Al in the asr._RNAi plants, indicating a central role for asr. in Al-responsive gene expression. Under normal conditions, without Al treatment, the asr. RNAi plants expressed 1.756 genes differentially compared to the wild-type plants, and 446 of these genes responded to AI treatment in the wild-type plants. Chromatin immunoprecipitation followed by deep sequencing identified 104 putative target genes that were directly regulated by asr. binding to their promoters, including the STAR1 gene, which encodes an ABC transporter required for AI tolerance. Motif analysis of the binding peak sequences revealed the binding motif for asr., which was confirmed via in vitro DNA-binding assays using the STAR1 promoter. These results demonstrate that asr. acts as a key transcription factor that is essential for AI-responsive gene expression and Al tolerance in rice.

关键词： Aluminum ChlP-Seq RNA-Seq rice asr.

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