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The Integrity of the Plant Golgi Apparatus Depends on Cell Growth-Controlled Activity of GNL1

Molecular Plant 2013年第3期6卷 905-915页

作者： Wenyan Du Kentaro Tamura Giovanni Stefano Federica Brandizzi MSU-DOE Plant Research Lab Michigan State University East Lansing MI 48824 USA Department of Plant Biology Michigan State University East Lansing MI 48824 USA Department of Botany Graduate School of Science Kyoto University Sakyo-ku Kyoto 606-8502 Japan

Membrane traffic and organelle integrity in the plant secretory pathway depend on arf-GTPases, which are activated by guanine-nucleotide exchange factors （arf-gefs）. While maintenance of conserved roles, evolution of unique functions as well as tissue-specific roles have been shown for a handful of plant arf-gefs, a fundamental yet unanswered question concerns the extent to which their function overlaps during cell growth. To address this, we have characterized pao, a novel allele of GNOM-like 1 （GNL1）, a brefeldin A （BFA）-insensitive arf-GEF, isolated through a confocal microscopy-based forward genetics screen of the Golgi in Arabidopsis thaliana. Specifically, we have analyzed the dependence of the integrity of trafficking routes and secretory organelles on GNL1 availability during expansion stages of cotyledon epidermal cells, an exquisite model system for vegetative cell growth analyses in intact tissues. We show that Golgi traffic is influenced largely by GNL1 availability at early stages of cotyledon cell expansion but by BFA- sensitive gefs when cell growth terminates. These data reveal an unanticipated level of complexity in the biology of GNL1 by showing that its cellular roles are correlated with cell growth. These results also indicate that the cell growth stage is an important element weighting into functional analyses of the cellular roles of arf-gefs.

关键词： arf-gefs Golgi Arabidopsis GNL1 brefeldin A.

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