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Dietary choline activates the ampk/Srebp signaling pathway and decreases lipid levels in Pacific white shrimp(Litopenaeus vannamei)

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Animal Nutrition 2023年第4期15卷 58-70页

作者： Jingjing Lu Xinyue Tao Jiaxiang Luo Tingting Zhu Lefei Jiao Peng Sun Qicun Zhou Douglas R.Tocher Min Jin Laboratory of Fish and Shellfish Nutrition School of Marine SciencesNingbo UniversityNingbo 315211China Guangdong Provincial Key Laboratory of Marine Biotechnology Institute of Marine SciencesShantou UniversityShantou 515063China

An 8-week feeding trial was conducted in Pacific white shrimp(Litopenaeus vannamei)to evaluate the effects of dietary choline supplementation on choline transport and metabolism,hepatopancreas histological structure and fatty acid profile,and regulation of lipid metabolism.Six isonitrogenous and isolipidic diets were formulated to contain different choline levels of 2.91(basal diet),3.85,4.67,6.55,10.70 and 18.90 g/kg,respectively.A total of 960 shrimp(initial weight,1.38±0.01 g)were distributed randomly into twenty-four 250-L cylindrical fiber-glass tanks,with each diet assigned randomly to 4replicate tanks.The results indicated that dietary choline significantly promoted the deposition of choline,betaine and carnitine(Pampk in hepatopancreas increased significantly as dietary choline increased(P<0.05).In addition,hepatopancreas m RNA expression levels of ctl1,ctl2,oct1,badh,bhmt,ck,cept,and cct were generally up-regulated as dietary choline level increased(P<0.01).In conclusion,dietary choline promoted the deposition of choline and its metabolites by up-regulating genes related to choline transport and metabolism.Moreover,appropriate dietary choline level promoted the development of hepatopancreas R cells and maintained the normal accumulation of lipids required for develo

关键词： Pacific white shrimp Choline Lipid Fatty acid Histology ampk signaling pathway

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MIR-448 Regulates MAGEA6/ampk signaling pathway in Hepatocellular Carcinoma Tumor Stem Cells

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Journal of Cancer Therapy 2023年第4期14卷 182-201页

作者： Changliang Jiao Jinfang Zheng Juncheng Guo Hainan Vocational University of Science and Technology Haikou China Hainan Provincial People’s Hospital Haikou China Haikou Hospital Xiangya School of Medicine Central South University Haikou China

Objective: To explore the role of miR-448 in regulating MAGEA6/ampk signaling pathway in the biological study of hepatocellular carcinoma (HCC) tumor stem cells. Methods: Using the database, the hepatocellular carcinoma related expression chips were obtained and the regulatory mirnas of candidate genes were predicted, and the predicted results were analyzed. The effects of miR-448 and MAGEA6 on the pellet formation rate and clone formation rate of hepatocellular carcinoma stem cells were detected by immunofluorescence identification of stem cell markers and light microscope counting method. The effects of miR-448 and MAGEA6 on migration and invasion of hepatocellular carcinoma stem cells were detected by scratch and Transwell assay. Dual luciferase reporter assay to verify whether miR-448 targets MAGEA6. The expression and influence of miR-448 on MAGEA6 and ampk pathway were detected by qRT-PCR and Western blot. Results: It was found that miR-448 may directly regulate the expression of MAGEA6. Overexpression of miR-448 inhibited the characteristics, proliferation, migration, and invasion of hepatocellular carcinoma stem cells in vitro, as well as the ability of xenograft tumor formation in vivo. However, inhibition of miR-448 showed opposite results. In addition, miR-448 directly targets MAGEA6 and regulates ampk signaling. Silencing MAGEA6 and adding ampk activator further verified that miR-448 activated ampk signaling pathway by targeting MAGEA6, thus affecting characteristics, proliferation, migration and invasion of hepatoma stem cells. Conclusions: Our results reveal that miR-448 activates ampk signaling pathway by targeting MAGEA6, thereby affecting characteristics, proliferation, migration and invasion of hepatoma stem cells. It is suggested that overexpression of miR-448 may be a new therapeutic strategy for hepatocellular carcinoma.

关键词： mir-448 MAGEA6 ampk signaling pathway Liver Cancer Tumor Stem Cells

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A new peptide,VD11,promotes structural and functional recovery after spinal cord injury

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Neural Regeneration Research 2023年第10期18卷 2260-2267页

作者： Shan-Shan Li Bai-Yu Zhang Sai-Ge Yin Zi-Qi Wei Nai-Xin Liu Yi-Lin Li Si-Yu Wang Yu-Heng Shi Jian Zhao Li-Juan Wang Yue Zhang Jun Sun Ying Wang Xin-Wang Yang Department of Anatomy and Histology&Embryology Faculty of Basic Medical ScienceKunming Medical UniversityKunmingYunnan ProvinceChina Key Laboratory of Chemistry in Ethnic Medicinal Resources&Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal Endophytes State Ethnic Affairs Commission&Ministry of EducationSchool of Ethnic MedicineYunnan Minzu UniversityKunmingYunnan ProvinceChina Department of Urology First Affiliated Hospital of Kunming Medical UniversityKunmingYunnan ProvinceChina Yunnan Province Clinical Research Center for Chronic Kidney Disease First Affiliated Hospital of Kunming Medical UniversityKunmingYunnan ProvinceChina

The regenerative capacity of the central nervous system is very limited and few effective treatments are currently available for spinal cord injury.It is therefore a priority to develop new drugs that can promote structural and functional recovery after spinal cord injury.Previous studies have shown that peptides can promote substantial repair and regeneration of injured tissue.While amphibians have a pronounced ability to regenerate the spinal cord,few studies have investigated the effect of amphibian spinal cord-derived peptides on spinal cord injury.Here we report for the first time the successful identification and isolation of a new polypeptide,VD11(amino acid sequence:VDELWPPWLPC),from the spinal cord of an endemic Chinese amphibian(Odorrana schmackeri).In vitro experiments showed that VD11 promoted the secretion of nerve growth factor and brain-derived neurotrophic factor in BV2 cells stimulated with lipopolysaccharide,as well as the proliferation and synaptic elongation of PC12 cells subjected to hypoxia.In vivo experiments showed that intravertebral injection of VD11 markedly promoted recovery of motor function in rats with spinal cord injury,alleviated pathological damage,and promoted axonal regeneration.Furthermore,RNA sequencing and western blotting showed that VD11 may affect spinal cord injury through activation of the ampk and AKT signaling pathways.In summary,we discovered a novel amphibian-derived peptide that promotes structural and functional recovery after spinal cord injury.

关键词： Akt signaling pathway amphibian-derived bioactive peptide ampk signaling pathway axonal regeneration brain-derived neurotrophic factor ischemia/reperfusion injury motor function nerve growth factor neuroprotective effect spinal cord injury

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Loganin regulates glycolipid metabolism by influencing intestinal microbiota and ampk signaling in obese mice

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Journal of Traditional Chinese Medical Sciences 2022年第3期9卷 321-329页

作者： Bingrui Xu Zimengwei Ye Tian Tian Ruyuan Zhu Chenyue Liu Xin Fang Dongwei Zhang Min Fu Sihua Gao Dandan Zhao School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing102488China Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing100700China Department of Endocrinology Beijing University of Chinese Medicine Third Affiliated HospitalBeijing100029China Research Institute of McGill University Health Center McGill UniversityMontrealH4A 3J1Canada

Objective: We aimed to observe the effects of loganin(Log) on serum glycolipid levels and probe the mechanisms focusing on intestinal flora and AMP-activated protein kinase(ampk) signaling in obese mice.Methods: A high-fat diet was given for 12 consecutive weeks to generate the obesity model in institute of cancer research(ICR) mice. Body weight was measured weekly and fasting blood glucose(FBG) was determined every 2 weeks. Both the oral glucose tolerance test and the intraperitoneal insulin tolerance test were performed. The serum levels of total cholesterol(TC), triglyceride, high-density lipoproteincholesterol, low-density lipoprotein-cholesterol(LDL-C), and free fatty acids(FFA) were measured. The expression of key proteins in the ampk signaling pathway in skeletal muscle tissue was detected by immunoblotting, and gut microbiota were characterized using 16S rDNA sequencing.Results: Log significantly decreased the body weight and the FBG in obese mice(P ampka), Sirtuin 1(SIRT1), and peroxisome proliferator-activated receptor-gamma coactivator-1 alpha(PGC1a) in skeletal muscle tissue of obese mice. 16S rDNA sequencing indicated that Log reduced the diversity of the gut flora in feces and altered the floral composition of obese mice.Conclusions: Log was effective in reducing body weight and improving glucolipid metabolism in obese mice, probably through activating ampk signaling and regulating intestinal microbial diversity.

关键词： Loganin Insulin resistance Obesity Glucose metabolism Lipid metabolism Gut microbiota ampk signaling pathway Mechanisms

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