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All-trans retinoic acid promotes smooth muscle cell differentiation of rabbit bone marrow-derived mesenchymal stem cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第7期11卷 489-496页

作者： Zhong-yuan SU Ying LI Xiao-li ZHAO Ming ZHANG College of Life Sciences Zhejiang University Hangzhou 310058 China Zhejiang Academy of Medical Science Hangzhou 310058 China

Bone marrow-derived mesenchymal stem cells are multipotent stem cells, an attractive resource for regenerative medicine. Accumulating evidence suggests that all-trans retinoic acid plays a key role in the devel- opment and differentiation of smooth muscle cells. In the present study, we demonstrate, for the first time, that rabbit bone marrow-derived mesenchymal stem cells differentiate into smooth muscle cells upon the treatment with all-trans retinoic acid. aii-trans retinoic acid increased the expression of myocardin, caldesmon, 22-kDa smooth muscle cell- specific protein （SM22Q）, and SM-myosin heavy chains in rabbit bone marrow-derived mesenchymal stem cells, as detected by reverse transcription polymerase chain reaction （PCR）. Immunostaining of SM22a and SM-myosin heavy chains using monoclonal antibodies also indicated smooth muscle cell differentiation of rabbit bone marrow- derived mesenchymal stem cells following the treatment with all-trans retinoic acid. In addition, more than 47% of bone marrow-derived mesenchymal stem cells demonstrated the contractile phenotype of smooth muscle cells. Western blot results showed that SM-1 and SM-2 were highly expressed in the differentiated cells. These results suggest that all-trans retinoic acid may serve as a potent agent for functional smooth muscle cell differentiation in tissue engineering.

关键词： Bone marrow-derived mesenchymal stem cells Smooth muscle cells aii-trans retinoic acid Rabbit

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