检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

土壤杆菌固氮、溶磷活性及对植物的促生作用

山东科学 2017年第4期30卷 26-30页

作者：魏艳丽 Maarten RYDER 李纪顺李玲杨玉忠杨合同山东省科学院生态研究所、山东省应用微生物重点实验室山东济南250014 澳大利亚阿德莱德大学农业食品葡萄酒学院阿德莱德5064 山东省黄河三角洲可持续发展研究院山东东营257000

通过选择性培养基,定性和定量检测了26株土壤杆菌的固氮和溶磷活性,通过盆栽检测了澳洲生防菌株K1026对黄瓜和小麦的促生长作用。结果显示,包括致病性和无致病性的26株土壤杆菌均可在无氮培养基上正常生长;其中19株具有溶磷活性,K1026... 详细信息

通过选择性培养基,定性和定量检测了26株土壤杆菌的固氮和溶磷活性,通过盆栽检测了澳洲生防菌株K1026对黄瓜和小麦的促生长作用。结果显示,包括致病性和无致病性的26株土壤杆菌均可在无氮培养基上正常生长;其中19株具有溶磷活性,K1026对磷酸钙液体培养基的溶磷量为109.11μg/m L;对黄瓜幼苗根系生长具有显著促进作用,但对小麦生长没有明显影响。

关键词：土壤杆菌 agrobacterium rhizogenes K1026 固氮溶磷促生作用

Cultivating Rice with Delaying Leaf-Senescence by P_(SAG12)-IPT Gene Transformation

在线全文

学校读者我要写书评

暂无评论

Acta Botanica Sinica 2002年第11期44卷 1333-1338页

作者：林拥军曹孟良徐才国陈浩魏君张启发华中农业大学作物遗传改良国家重点实验室分子生物学分室武汉430070

P SAG12 _ IPT gene was introduced into an elite rice (Oryza sativa L. ssp. indica ) restorer line Minghui 63 through agrobacterium _mediated transformation method. Out of 61 independent transgenic plants obtained, a few acquired a recognizable phenotype in which leave senescence was delayed to a great degree. The results of field plot test on two homozygous transgenic lines indicated: (1) the stay_green ability of transgenic plants was significantly improved; (2) both the seed_setting rate and the number of panicles per plant of transgenic plants were significantly increased compared with that of the non_transgenic plants of Minghui 63; and (3) the plant height of transgenic plants was significantly reduced.

关键词： Oryza sativa agrobacterium transformation P SAG12 _ IPT stay_green agronomic traits

Production of the Cancer-Preventive Glucoraphanin in Tobacco

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2010年第4期3卷 751-759页

作者： Michael Dalgaard Mikkelsen Carl Erik Olsen Barbara Ann Halkier University of Copenhagen Faculty of Life Sciences Department of Plant Biology Plant Biochemistry Laboratory VKR Research Centre for Pro-Active Plants 40 Thorvaldsensvej DK-1871 Frederiksberg C Copenhagen Denmark University of Copenhagen Faculty of Life Sciences Department of Basic Sciences and Environment/Bioorganic Chemistry 40 Thorvaldsensvej DK-1871 Frederiksberg C Copenhagen Denmark

Epidemiological studies have demonstrated reduced risk of developing cancer upon consumption of diets rich in cruciferous vegetables. This chemoprevention has been largely attributed to the presence of the natural products glu- cosinolates, particularly the methionine-derived glucoraphanin from broccoli. Improved nutrition by functional foods or health-promoting dietary supplements is an attractive means for prevention of lifestyle-based diseases. Towards this goal, we have engineered the glucoraphanin pathway into tobacco. First, we engineered elongation of the side chain of methionine to produce the key intermediate dihomo-methionine. This process is catalyzed through two cycles in a chain-elongation pathway that takes place partly in the cytosol and partly in the chloroplast. Second, by coupling the five enzymes of the chain-elongation pathway to eight enzymes of the glucosinolate pathway, we show production of glucoraphanin together with other glucosinolates derived from chain-elongated isoleucine and/or leucine. The conver- sion of methionine to glucoraphanin is obtained via 14 intermediates. Demonstrating the production of the high-value glucoraphanin in a heterologous host has great potential in the food and medicinal industry as a means to generate a stable, rich source of glucoraphanin for the benefit of human health.

关键词： Compartmentation secondary metabolism/natural products agrobacterium Arabidopsis tobacco glucosinolates metabolic engineering.

Expression of the Arabidopsis thaliana Histone Gene AtHTA1 Enhances Rice Transformation Efficiency

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2009年第4期2卷 832-837页

作者： Ye Zheng Xiao-Wei He Ying-Hui Ying Jiang-Feng Lu Stanton B. Gelvin Hui-Xia Shou State Key Laboratory of Plant Physiology and Biochemistry College of Life Sciences Zhejiang University Yuhangtanghe Road 368 Hangzhou 310058 China Department of Biological Sciences Purdue University West Lafayette IN 47907-1392 USA

We expressed the Arabidopsis thaliana histone AtHTA1 in rice under the control of the maize ubiquitin promoter. Transformation efficiencies of rice plants that constitutively expressed AtHTA 1 were 28-44% higher than calli containing an empty vector control. Furthermore, co-infection of rice calli with a vector containing AtHTA 1 and another vector with the target gene increased transformation by 27-50%. Thus, expression of AtHTA 1 either transiently or in stably transformed cells improved rice transformation efficiency.

关键词： Arabidopsis histone H2A agrobacterium rice transformation.

Expression of a Rice Chitinase Gene Enhances Antifungal Response in Transgenic Litchi (cv. Bedana)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2018年第11期9卷 2256-2275页

作者： Dilip Kumar Das Amir Rahman Post Graduate Department of Biotechnology Tilka Manjhi Bhagalpur University Bhagalpur India

To enhance the antifungal response of litchi, transferring rice chitinase gene under a maize-ubiquitin promoter along with its first intron into the zygotic embryos via agrobacterium tumefaciens-mediated transformation generated transgenic plants. After co-cultivation for 2 days with recombinant agrobacterium, zygotic embryos were transferred onto Murashige and Skoog (MS) medium consisted of MS salts and Gamborg (B5) vitamins with 2 mgl-1 2, 4-dichlorophenoxyacetic acid (2, 4-D), 50 gl-1 sucrose and 8 gl-1 agar supplemented with 25 mgl-1 hygromycin and 400 mgl-1 cefotaxime. Embryos were selected passing through a series of MS modified media and the antibiotic resistant transgenic plantlets were analyzed. The integration and stability of the transgene was confirmed by PCR, RT-PCR, Southern blotting and by Western blot analyses. The transgenic plants exhibited higher chitinase activity than the non-transformed plants. The chitinase activity was also examined using the native polyacrylamide in-gel assay. These analyses indicated that the foreign gene was translated into the protein of expected molecular weight that showed chitinase activity. Following in-vitro inoculation of die-back, leaf spots and blight pathogen (Phomopsis sp.), the transgenic plants showed delayed onset of the disease and smaller lesions. The transgenic plants were adapted to the greenhouse and did not show any phenotypic alterations.

关键词： Transgenic Litchi agrobacterium Tumefaciens Blight Die Back and Leaf Spots Phomopsis sp. Somatic Embryogenesis Transformation

Development of High Efficient in vitro Regeneration and Transformation System in Strawberry

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Northeast Agricultural University(English Edition) 2018年第2期25卷 1-12页

作者： Li Wen-yan Zhou Jing Wei You Wei Chi-zhang South Asian Tropical Agricultural Science Research Institute of Guangxi Longzhou 532415 Guangxi China

In this paper, several factors that affect the efficiency of in vitro adventitious bud regeneration and agrobacterium tumefaciens-mediated transformation of F. vesca were studied. The results showed that F. vesca seeds germination rate was the highest while seeds were cultured in water, and the germination rate was the lowest while seeds were cultured on MS medium supplemented with hormone; the germination rates that seeds cultured on two and three layers filter paper were higher than that seeds cultured on four and five layers filter paper. In vitro adventitious regeneration efficiency was affected by different explants types. The significant difference was existed between petioles and leaves. When using the same type explants, in vitro adventitious buds regeneration rate and the average number of buds per explant between Ruegen （RE） and Yellow Wonder （YW） had no significant difference. RE to agrobacterium tumefaciens was more sensitive than YW. Using seedling leaves of RE and YW as materials, an efficient agrobacterium-mediated transformation system was developed. In this system, the concentration of bacteria was OD600=0.5, the explants were immersed in bacteria broth for 9 min, the co-cultured time was 2 days, and had no pre-cultured time. The percentage of explants with resistant buds of RE and YW was compared. The putative transformed plants were confirmed by PCR.

关键词： F vesca in vitro regeneration agrobacterium genetic transformation

Deletional analysis of functional regions of complementary sense promoter from cotton leaf curl virus

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2000年第5期43卷 498-506,562页

作者：谢迎秋刘玉乐朱祯 Laboratory of Plant Genetic Manipulation Institute of Genetics Chinese Academy of Sciences Beijing 100101 China Laboratory of Plant Genetic Manipulation Institute of Genetics Chinese Academy of Sciences Beijing 100101 China Institute of Microbiology Chinese Academy of Sciences Beijing 100080 China

Complementary sense promoter from cotton leaf curl virus (CLCuV) is a novel plant promoter for genetic engineering that could drive high-level foreign gene expression in plant. To determine the optimal promoter sequence for gene expression, CLCuV promoter was deleted from its 5' end to form promoter fragments with five different lengths, and chimeric gus genes were constructed using the promoterdeletion. These vectors were delivered into agrobacterium and tobacco (Nicotiana tabacum L cv. Xanthi) plants which were transformed by leaf discs method. GUS activity of transgenic plants was measured. The results showed that GUS activities with the promoter deleted to -287 and -271 from the translation initiation site were respectively about five and three times that of full-length promoter. There exists a c/s-element which is important for the expressing activity in phloem from -271 to -176. Deletion from -176 to -141 resulted in a 20-30-fold reduction in GUS activity in leaves with weak activity in leaves and stems and losing GUS activity in roots. The functional domains of complementary sense gene promoter of CLCuV were firstly analyzed and compared. It was found that the promoter activity with the deletion of negative c/s-elements was much stronger than that of full-length promoter and was about twelve times on average that of CaMV 35S promoter, suggesting that the promoter has great application potential. Results also provide novel clues for understanding the mechanisms of geminivirus gene regulation and interaction between virus and plant.

关键词： geminivirus promoter cis-element agrobacterium tobacco.

Cloning of Three Antiporter Genes from Arabidopsis and Rice for Over-Expressing Them in Farmer Popular Tomato Varieties of Bangladesh

在线全文

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2014年第26期5卷 3957-3963页

作者： Samsad Razzaque Debashis Chakraborty Rumana Sultana Tammi Sabrina M. Elias Zeba I. Seraj Aparna Islam Plant Biotechnology Lab Department of Biochemistry and Molecular Biology University of Dhaka Dhaka Bangladesh Biotechnology Program Department of Mathematics and Natural Sciences BRAC University Dhaka Bangladesh

Salinity is one of the most critical environmental problems, which causes plant growth retardation by disturbing intracellular ion homeostasis. The Na+/H+ antiporter plays an important role in resistance to salt stress by sequestering Na+ in exchange for H+ across the vacuolar membranes. In the current study, the coding regions of two Arabidopsis antiporters (AtNHX1 and AtNHX2) and one rice antiporter (OsNHX1) were amplified by target specific PCR. PCR amplicons were first cloned into pENTR/D-TOPO and later recombined with a destination vector (pK7WG2.0) by LR reaction. Positive clones were selected by PCR, restriction digestion (RD) and sequencing. They were then transformed into agrobacterium tumefaciens (LBA4404 strain) for subsequent transformation of farmer popular tomato varieties.

关键词： Antiporters pENTR/D-TOPO pK7WG2.0 agrobacterium

灵芝甾醇14α-脱甲基酶基因的克隆及超量表达对三萜合成的影响

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

菌物学报 2011年第2期30卷 242-248页

作者：方星师亮徐颖洁赵明文南京农业大学生命科学学院农业部农业微生物环境工程重点开放实验室南京210095 南京农业大学作物遗传与种质创新国家重点实验室南京210095

灵芝Ganoderma lucidum是我国传统的药用真菌,三萜类物质是灵芝的主要生物活性成分,甾醇14α-脱甲基酶是三萜合成途径中的关键酶。根据已报道其他物种甾醇14α-脱甲基酶的氨基酸保守序列设计简并引物,获得灵芝甾醇14α-脱甲基酶特异基... 详细信息

灵芝Ganoderma lucidum是我国传统的药用真菌,三萜类物质是灵芝的主要生物活性成分,甾醇14α-脱甲基酶是三萜合成途径中的关键酶。根据已报道其他物种甾醇14α-脱甲基酶的氨基酸保守序列设计简并引物,获得灵芝甾醇14α-脱甲基酶特异基因片段,并进一步获得灵芝甾醇14α-脱甲基酶基因的全长DNA和cDNA序列。其中DNA序列长1,981bp,cDNA序列长1,635bp。结构基因编码蛋白包含544个氨基酸,分子量为61.99kDa,等电点为6.36。将甾醇14α-脱甲基酶基因的cDNA序列克隆到灵芝超量表达载体pGl-GPD中,利用农杆菌介导的转化法实现了甾醇14α-脱甲基酶基因在灵芝内的超量表达。转化子的甾醇14α-脱甲基酶基因在转录水平表达量增加,三萜含量增加。进一步研究发现,三萜合成途径的关键酶基因Gl-aact、Gl-hmgr及Gl-ls的转录表达量也有所增加。

关键词：灵芝 agrobacterium tumefaciens介导转化超量表达三萜合成