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Bioactive extracts of red seaweeds Pterocladiella capillacea and Osmundaria obtusiloba(Floridophyceae:Rhodophyta) with antioxidant and bacterial agglutination potential

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Asian Pacific Journal of Tropical Medicine 2016年第4期9卷 363-370页

作者： Daniel Barroso de Alencar Fátima Cristiane Teles de Carvalho Rosa Helena Rebouas Daniel Rodrigues dos Santos Kelma Maria dos Santos Pires-Cavalcante Rebeca Larangeira de Lima Bárbara Mendes Baracho Rayssa Mendes Bezerra Francisco Arnaldo Viana Regine Helena Silva dos Fernandes Vieira Alexandre Holanda Sampaio Oscarina Viana de Sousa Silvana Saker-Sampaio Laboratrio de Produtos Naturais Marinhos.Departamento de Engenharia de Pesca Universidade Federal do Ceará.Campus do Pici.Ar.Mister Hull.s/nCaixa Postal 6043 Laboratrio de Microbiologia Ambiental e do Pescado.Instituto de Ciências do Mar.Universidade Federal do Ceará.Av.da Abolicao 3207 Laboratrio de Microbiologia Ambiental e do Pescado.Instituto de Ciências do Mar.Universidade Federal do Ceará Laboratrio de Produtos Naturais Marinhos.Departamento de Engenharia de Pesca Universidade Federal do Ceará Laboratrio de Cromatografia Departamento de QuimicaUniversidade do Estado do Rio Grande do NorteCampus Universitário Central

Objective:To evaluate the antioxidant,antibacterial and bacterial cell agglutination activities of the hexane(Hex) and 70%ethanol(70%EtOH) extracts of two species of red seaweeds Pterocladiella capillacea(***) and Osmundaria ***:In vitro antioxidant activity was determined by DPPH radical scavenging assay,ferric-reducing antioxidant power assay,ferrous ion chelating assay,β-carotene bleaching assay and total phenolic content *** activity was tested using the method of disc diffusion on Mueller-Hinton *** ability of algal extracts to agglutinate bacterial cells was also ***:The 70%EtOH extract of the two algae showed the highest values of total phenolic content compared to the Hex *** results of DPPH for both extracts(Hex,70%EtOH) of Osmundaria obtusiloba(43.46%and 99.47%) were higher than those of ***(33.04%and 40.81%) at a concentration of 1 000 μg/*** for the ferrous ion chelating,there was an opposite behavior,extracts of *** had a higher *** extracts showed a low ferric-reducing antioxidant power,with optical density ranging from0.054 to *** activities of all extracts evaluated for β-carotene bleaching were above 40%.There was no antibacterial activity against bacterial strains ***,the extracts of both species were able to agglutinate bacterial Gram positive cells of Staphylococcus aureus and Gram negative cells of Escherichia coli,multidrug-resistant Salmonella and Vibrio *** This is the first report of the interaction between these algal extracts,rich in natural compounds with antioxidant potential,and Gram positive and Gram negative bacterial cells.

关键词： Bioactive compounds Antioxidant Bacterial agglutination Rhodophyta

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The Expression and Characterization of a Bifunctional Protein in *** for Autologous Erythrocyte agglutination Test

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Cellular & Molecular Immunology 2008年第4期5卷 299-306页

作者： Changli Shao Jingang Zhang Institute of Transfusion Medicine Academy of Military Medical SciencesBeijing 100850 China Biochemistry Department Chinese PLA General Hospital Beijing 100853China

H antigen, the precursor of A and B antigens, belongs to Hh blood system in which it is the only antigen. H antigen distributes on all the human RBC surface except for Bombay phenotype and the copy number of H antigen on the surface of an adult RBC is approximately 1.7 × 10^6. These characteristics made H antigen the potential target molecule for the immunoassay and immunotherapy. A monoclonal antibody 2E8 against H antigen on the surface of erythrocyte had been prepared in previous work. Based on this antibody, the variable region genes of heavy and light chains （Vu and VL） from 2E8 had been cloned by 5＇ RACE. The two variable region genes were spliced by overlap extension and assembled ScFv （Vu-linker-VL） gene encoding the anti-H antigen named ScFv2Es. According to the prediction of the three-dimension structure of ScFv2Es and Cul fragment from 2E8 and HIV-1 gp41 antigen peptide, we further constructed the ScFv2Es-Cul-gp41 fusion molecule. The recombinant ScFv2Es-Cul-gp41 gene was cloned into pET-his vector and expressed in BL21（DE3）plysS cells. The fusion protein was purified from the inclusion bodies. In a series of subsequent analyses, this fusion protein showed identical antigen binding site and activity with the parent antibody. Meanwhile, in mimic test, as the main ingredient of reagent for autologous erythrocyte agglutination test, the bifunctional protein could agglutinate the RBCs in the presence of HIV-1 gp41 antibodies using sera from HIV-infected individuals. Cellular ＆ Molecular Immunology. 2008;5（4）：299-306.

关键词： agglutination bifunctional protein

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Severe Labial agglutination Release in a Postmenopausal Woman

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Open Journal of Obstetrics and Gynecology 2014年第14期4卷 822-825页

作者： Rebecca James Anne Sammarco David Sheyn Sangeeta Mahajan Department of Obstetrics and Gynecology University Hospitals Case Medical Center Cleveland USA

Labial agglutination occurs when the labia minora have become fused in the midline through either filmy or dense adhesions, forming a raphe. This condition is typically seen in pre-pubertal girls;however, there have been cases reported in the literature of labial agglutination in postmenopausal and reproductive aged women. Labial agglutination has been associated with local irritation and inflammation in the setting of a hypoestrogenic state. Topical treatment is usually recommended prior to manual separation. However, some postmenopausal women have been refractory to conservative management, and surgery may then be required. We present a case of a 79-year-old Caucasian gravida 3 para 3 who was referred for treatment refractory labial agglutination for 3 years. The patient’s complaints included a small trickle escaping from the introitus during urination and the sensation of little urinary control. The patient underwent an exam under anesthesia. Gentle blunt dissection with a lacrimal dilator easily separated the labia minora down the visible midline fusion line (raphe). Bimanual exam then revealed a 12-week-sized enlarged uterus confirmed on ultrasound to be an 8 × 8 × 7 cm irregular mass concerning for uterine cancer. Proceeding to an operative release of severe labial agglutination can relieve patient discomfort and frustration when estradiol therapy has failed. In addition to alleviating symptoms, releasing labial agglutination can also provide the gynecologist access to critical screening procedures such as cervical cytology or a bimanual exam, all of which could lead to a diagnosis or pre-cancerous or cancerous lesions.

关键词： Labial agglutination Hypoestrogenic State

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Preparation and evaluation of a glycerol-preserved direct agglutination antigen for long-term preservation:a comparative study of the detection of anti-Leishmania infantum antibodies in human and dog

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Asian Pacific Journal of Tropical Medicine 2012年第2期5卷 117-120页

作者： Behnaz Akhoundi Mehdi Mohebali Gholam-Hossein Edrissian Mohammad-Bagher Eslami Hossein Keshavarz Hossein Malekafzali Mohammad-Bagher Rokni Department of Medical Parasitology and Mycology School of Public HealthTehran University of Medical SciencesTehranIran Department of Pathobiology School of Public HealthTehran University of Medical SciencesTehranIran Department of Biostatistics and Epidemiology School of Public HealthTehran University of Medical SciencesTehranIran

Objective:To prepare and evaluate a glycerol-preserved antigen from an Iranian strain of Leishmania infantum(L infantum) for use in glycerol-preserved direct agglutination tests (GP-DAT) as an alternative to freeze dried direct agglutination teals(FD-DAT) that use freezedried ***:Glycerol-preserved DAT antigen was prepared and stored at different *** tested antigen stored at 4℃,22-37℃and 50℃over a period of 365 days. Seven hundred twenty-nine serum samples were collected from different geographical zones of Iran from 2007-2009,and 80 of these samples were pooled to produce *** pooled serum contained 10 *** positive and negative pooled sera were separately tested for anti-L. infantum antibodies with GP-DAT,FD-DAT and formaldehyde-fixed direct agglutination test (FF-DAT) antigens;tests were performed on both human and dog sera over a period of 12 months. Results:There was strong agreement between the results obtained using GP-DAT and FDDAT antigens stored at 22-37℃for 12 months for both human(100%) and dog(100%) pooled *** direct agglutination test results were highly reproducible(weighted kappa:GP=0.833, FD=0.979 and FF=0.917).Conclusions:Because GP-DAT antigen is highly stable over a range of temperatures and is easy to transport in the field,this type of antigen may be particularly useful in areas with endemic visceral leishmaniasis.

关键词： Leishmania infantum Direct agglutination test antigen Preservation Glycerol Iran

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Detection of leptospiral antibodies by microscopic agglutination test in north-east of Iran

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Asian Pacific Journal of Tropical Biomedicine 2011年第3期1卷 227-229页

作者： Ehsanollah Sakhaee Gholam Reza Abdollah pour Department of Clinical Sciences Faculty of Veterinary MedicineShahid Bahonar University of Kerman Department of Clinical Sciences Faculty of Veterinary MedicineUniversity of Tehran

Objective:To detect leptospiral antibodies by microscopic agglutination test(MAT) in northeast of ***:This study was conducted to evaluate prevalence of human leptospiral infections by *** six current reference strains of Leptospira interrogans in north-east of Iran.A total of 285 serum samples were collected from three north-east provinces of Iran,from December,2009 to June,***:Antibodies were detected at least against one serovar of Leptospira interrogans in 45 sera(15.79%) among 283 samples at a dilution 1:100 or *** titers against more than one serovar were detected in 24 sera of the positive ***, there were 75 positive reactions against different serovar of Leptospira *** titers were recorded against serovar icterohaemorrhagiae(31 samples),hardjo(26 samples), grippotyphosa(7 samples),pomona(5 samples),canicola(4 samples) and ballum(***). Conclusions:In present study the most prevalent(Leptospira icterohaemorrhagiae) and the least prevalent(Leptospira ballum) serovar are different from previous ***,species and prevalence of serovars change during the lime in one area and between regions.

关键词： Leptospirosis Microscopic agglutination test Human Iran Prevalence Leptospiral infection Serovar

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A rapid, simple, and sensitive immunoagglutination assay with silica nanoparticles for serotype identification of Pseudomonas aeruginosa

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Journal of Integrative Agriculture 2019年第8期18卷 1936-1943页

作者： DONG Xiu-mei TAO Jing LI Ting-ting ZHANG Ping ZHU Yan TANG Yu SU Rui-hong SHI Dong-fang College of Veterinary Medicine Northeast Agricultural University Harbin 150030 P.R.China Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development College of Veterinary MedicineNortheast Agricultural University Harbin 150030 P.R.China

An agglutination test based on colored silica nanoparticles(colored SiNps) was established to detect serotypes of Pseudomonas aeruginosa. Monodisperse colored SiNps were used as agglutination test carriers. The colored SiNps were prepared through reverse microemulsion with reactive dyes, sensitized with 11 kinds of mono-specific antibodies against P. aeruginosa, and denoted as IgG-colored SiNps. Eleven kinds of IgG-colored SiNps were individually mixed with P. aeruginosa on a glass *** serotypes of P. aeruginosa could be identified by agglutination test with evident agglutination. The P. aeruginosa could be detected in a range from 3.6×10^5 to 3.6×10^12 cfu mL^–1. This new agglutination test was confirmed to be a specific,sensitive, fast, easy-to-perform, and cost-efficient tool for the routine diagnosis of P. aeruginosa.

关键词： Pseudomonas aeruginosa colored silica nanoparticles agglutination test serotype detection

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Characterization and Phylogenetic Analysis of Lectin Gene cDNA Isolated from Sea Cucumber (Apostichopus japonicus) Body Wall

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Journal of Ocean University of China 2017年第6期16卷 1148-1156页

作者： XUE Zhuang LI Hui LIU Yang ZHOU Wei SUN Jing WANG Xiuli College of Fisheries and Life Science Dalian Ocean University Dalian 116023 P. R. China Liaoning Province Academy of Analytic Science Shenyang 110015 P. R. China

As a ‘living fossil' of species origin and ‘rich treasure' of food and nutrition development, sea cucumber has received a lot of attentions from researchers. The cDNA library construction and EST sequencing of blood had been conducted previously in our lab. The bioinformatic analysis provided a gene fragment which is highly homologous with the genes of lectin family, named AjL(Apostichopus japonicus lectin). To characterize and determine the phylogeny of AjL genes in early evolution, we isolated a fulllength cDNA of lectin gene from the body wall of A. japonicus. The open reading frame of this gene contained 489 bp and encoded a 163 amino acids secretory protein being homologous to lectins of mammals and aquatic organisms. The deduced protein included a lectin-like domain. SDS-PAGE analysis showed that AjL migrated as a specific band(about 36.09 kDa under reducing), and agglutinated against rabbit red blood cells. AjL was similar to chain A of CEL-IV in space structure. We predicted that AjL may play the same role of CEL-IV. Our results suggested that more than one lectin gene functioned in sea cucumber and most of other species, which was fused by uncertain sequences during the evolution and encoded different proteins with diverse functions. Our findings provided the insights into the function and characteristics of lectin genes invertebrates. The results will also be helpful for the identification and structural, functional, and evolutionary analyses of lectin genes.

关键词： sea cucumber agglutination activity lectin space structure

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Label-free colorimetric estimation of proteins using nanoparticles of silver

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Nano-Micro Letters 2010年第3期2卷 164-168页

作者： Siddhartha Shrivastava Debabrata Dash Department of Biochemistry Institute of Medical SciencesBanaras Hindu University

Metallic nanoparticles have received considerable attention in bioassays and diagnostics due to their unique surface plasmon resonance(SPR) *** nanoparticles have been employed for the development of SPR-based colorimetric *** the present report we have described a sensitive colorimetric approach for estimation of proteins,within a detection limit of 10?80 μg/m L,using unmodified silver *** the common advantages of colorimetric assay such as simplicity,high sensitivity,and low cost,our method has a label-free design and provides an important and attractive alternative to classical sensing probes and *** present work will contribute to the development of nanotechnology-based diagnostic tools.

关键词： Silver nanoparticles agglutination Surface plasmon resonance Colorimetric estimation Bovine serum albumin Immunoglobulin

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Clinical profile of brucellosis from a tertiary care center in southern India

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Asian Pacific Journal of Tropical Medicine 2011年第5期4卷 397-400页

作者： Vishwanath Sathyanarayanan Abdul Razak Kavitha Saravu Shastry Barkur Ananthakrishna Mukhyprana Prabhu M Vandana KE Department of Internal Medicine Kasturba Medical CollegeManipal UniversityKarnatakaIndia Department of Microbiology Kasturba Medical CollegeManipal UniversityKarnatakaIndia

Objective:To highlight the spectrum of clinical manifestations,labs,complications,treatment and outcome of ***:Retrospective study was conducted in Kasturba Medical College,Manipal University,Karnataka,India which included 68 confirmed cases of brucellosis from January 2006- April *** of brucellosis was made by culturing the sera/body fluids by standard BACTEC method(or) by testing the sera for Brucella agglutinins using the standard agglutination test(SAT).A titer of 1:320 or more was considered as *** 16 was used for statistical analysis and Microsoft Excel for graphical ***:Of the 68 patients,46(68%) were male and 22(32%) were female patients with age distribution of 9-75 *** four(64.7%) had history of contact with unpasteurized dairy products or infected *** included fever(68,100%),myalgia(21,31%),musculoskeletal symptoms (23,34%),headache(16,24%),gastrointestinal symptoms(19,28%) and altered sensorium(3, 4%).Co-morbidities and associations included HIV positivity(2,3%),type 2 diabetes mellitus (13,19%),steroid therapy(3,4%) and HBsAg positivity(8,12%).Ten(15%) patients had cervical lymphadenopathy,4(6%) had splenic enlargement,6(7%) had hepatomegaly,19(28%) had hepatosplenomegaly and 2(3%) got meningeal *** was observed in 39(57.3%) cases,high erythrocyte sedimentation rate(ESR) was present in 55(80.8%) cases,leucocytosis in 10(14.7%),leucopenia in 10(14.7%),thrombocytopenia in 23(33.82%) and thrombocytosis in 2(2.94%) ***:In countries like India,where brucellosis and tuberculosis are endemic;rapid,sensitive and highly specific diagnostic methods are required to make early diagnosis and prevent resistance as there is an overlap in therapy.

关键词： Brucella South India BACTEC Standard agglutination test

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COUNTERCURRENT IMMUNOELECTROPHORESIS FOR DIAGNOSIS OF ACUTE BACTERIAL PNEUMONIA IN CHINESE CHILDREN

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中华医学杂志(英文版) 1993年第2期106卷 105-109页

作者：杨永弘江载芳陈贤楠江沁波张桂荣沈叙庄彭华 Rachel Schneerson John B.Robbins Laboratory of Microbiology and Immunology Beijing Pediatric Instituteand Pulmonary Division and ICUBeijing Children's HospitalBeijing 100045 Laboratory of Developmental and Molecular Immunity National Institute of Child Health and DevelopmentNational Institutes of HealthBethesdaUSA

Countercurrent immunoelectrophoresis(CIE)with *** type b(Hib) and Pneumococci Omni antisera was per-formed on serum and concentrated urine and pleural fluid sam-ples from 100 patients with acute pneumonia in BeijingChildren's ***-one patients were investigated bybacteriologic techniques(blood culture and pleural fluidculture).CIE was positive in 29/100 (29%) of cases for Hib,13/100 (13%) of cases for ***,bacterialcauses of pneumonia were diagnosed by CIE in 41/100 (41%)*** were detected in 5/90 (5.6%) serum samples,in 36/87(41.5%) concentrated urine samples,and in 1/2(50%) samples of pleural *** one Hib strain was foundby pleural fluid *** contamination rate of bacterial cul-ture in this group was high (5/31 cases,16.1%).Therefore,traditional bacteriologic techniques are of very limited valuefor diagnosing pneumonia in Chinese *** results show-ed that bacterial pathogen,especially Hib and Pneumococci,are very common and important causes of pediatric pneumoniain China.

关键词： INFLUENZAE TYPE-B COUNTERIMMUNOELECTROPHORESIS agglutination ANTIGENS WORLD

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