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灭菌过程中AGEs和ALEs的形成及抑制

吉林农业大学学报 2022年第5期44卷 541-547页

作者：王婧朴春红王玉梅刘俊梅胡洋唐玉芳吉林农业大学食品科学与工程学院长春130118

在巴氏杀菌和高温高压灭菌条件下,研究葡萄糖/果糖-BSA,亚油酸-赖氨酸/BSA和豆乳中晚期糖基化终末产物(Advanced glycation end products,AGEs)或晚期脂质过氧化终产物(Advanced lipid peroxidation end products,ALEs)形成情况及荞麦... 详细信息

在巴氏杀菌和高温高压灭菌条件下,研究葡萄糖/果糖-BSA,亚油酸-赖氨酸/BSA和豆乳中晚期糖基化终末产物(Advanced glycation end products,AGEs)或晚期脂质过氧化终产物(Advanced lipid peroxidation end products,ALEs)形成情况及荞麦壳提取物(Buckwheat hull extract,BHE)对其的抑制作用,以氨基胍(Amino guanidine,AG)作为阳性对照。结果表明:在不同的体系灭菌过程中均形成了大量的AGEs或ALEs。在葡萄糖/果糖-BSA体系中BHE呈现出高抑制AGEs活性,最高抑制率达到66.55%(80℃,1 h),其抑制方式与AG不同。在亚油酸-赖氨酸/BSA体系中,BHE对其ALEs的形成抑制效果也高于AG,但其抑制率低于葡萄糖/果糖-BSA体系,最高抑制率为39.14%(80℃,6 h)。加入BHE豆乳经灭菌后,其荧光值与未加入的豆乳相比,均显著性下降,下降幅度为11.78%(80℃,1 h)、14.32%(115℃,20 min)和14.09%(121℃,20 min)。荞麦壳提取物能显著降低食品加工中AGEs和ALEs的形成,在低褐变食品加工中具有重要意义。

关键词：荞麦壳提取物美拉德反应 AGEs ALEs 巴氏杀菌高温高压灭菌

PM2.5 exposure induces age-dependent hepatic lipid metabolism disorder in female mice

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Journal of Environmental Sciences 2020年第3期32卷 227-237页

作者： Ruifeng Yan Tingting Ku Huifeng Yue Guangke Li Nan Sang College of Environment and Resource Research Center of Environment and HealthShanxi UniversityTaiyuanShanxi 030006China

Particulate matter exposure has been described to elevate the risk of lung and cardiovascular diseases.An increasing number of recent studies have indicated positive correlations between PM2.5(the fraction of airborne particles with an aerodynamic diameter less than2.5 μm) exposure and the risk of liver diseases.However,research on the effects of PM2.5exposure on liver fat synthesis,secretion,and clearance mechanisms under normal diet conditions is limited,and whether these effects are age-dependent is largely unknown.Female C57BL/6 mice at different AGEs(4 weeks(4 w),4 months(4 m),and 10 months(10 m))were treated with 3 mg/kg body weight of PM2.5 every other day for 4 weeks.Subsequently,the ultrastructural changes of liver,the expression of genes involved in oxidative damage and lipid metabolism in the liver were examined.Observation of hepatic ultrastructure showed more and larger lipid droplets in the livers of 4-week-old and 10-month-old mice exposed to PM2.5.Further analysis showed that PM2.5 exposure increased the expression of genes related to lipid synthesis,but decreased the expression of genes involved in lipid transport and catabolism in the livers of 10-month-old mice.Our findings suggest that exposure to PM2.5 disrupts the normal metabolism of liver lipids and induces lipid accumulation in the liver of female mice in an age-dependent manner,with older mice being more susceptible to PM2.5.

关键词： Fine particulate matter Liver Lipid metabolism Different AGEs

Focusing on the recent progress of tea polyphenol chemistry and perspectives

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Food Science and Human Wellness 2022年第3期11卷 437-444页

作者： Shiming Li Liang Zhang Xiaochun Wan Jianfeng Zhan Chi-Tang Ho Hubei Key Laboratory of EFGIR Huanggang Normal UniversityHuanggang 438000China State Key Laboratory of Tea Plant Biology and Utilization School of Tea&Food ScienceAnhui Agricultural UniversityHefei 230036China Department of Food Science Rutgers UniversityNJ 08901USA

Myriad evidence attests to the health-promoting benefits of tea drinking.While there are multiple factors of tea influencing the effective biological properties,tea polyphenols are the most significant and valuable components.The chemical characterization and physical characteristics of tea polyphenols have been comprehensively studied over the previous years.Still the emergence of new chemistry in tea,particularly the property of scavenging reactive carbonyl species(RCS)and the newly discovered flavoalkaloid compounds,has drawn increasing attention.In this review,we summarize recent findings of a new class of compounds in tea-flavonoid alkaloids(flavoalkaloids),which exist in fresh tea leaves and can be generated during the process of post-harvesting,and also postulate the formation mechanism of flavoalkaloids between catechins and theanine-derived Strecker aldehyde.Additionally,we detail the up-to-date research results of tea polyphenols regarding their ability to trap RCS and their in vivo aminated metabolites to suppress advanced glycation ends products(AGEs).We further raise questions to be addressed in the near future,including the synthetic pathways for the generation of flavoalkaloids and AGEs in fresh tea leaves before processing and the concentrations of tea polyphenols that affect their RCS scavenging capability due to their pro-oxidant nature.More intensive research is warranted to elucidate the mechanisms of action underlying the biological activity of flavoalkaloids and the pharmacological application of tea polyphenols in scavenging RCS and impeding detrimental AGEs.

关键词： Tea chemistry Flavonoalkaloids RCS adducts Aminated tea polyphenols AGEs

The significance of U-Pb zircon AGEs in zoned plutons:the case of the Flamenco pluton, Coastal Range batholith, northern Chile

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Geoscience Frontiers 2019年第3期10卷 1073-1099页

作者： Natalia Rodríguez Juan Díaz-Alvarado Carlos Fernández Paulina Fuentes Christoph Breitkreuz Colombo Celso Gaeta Tassinari Departamento de Geología Universidad de Atacama Departamento de Ciencias de la Tierra Universidad de Huelva Institut für Geologie TU Bergakademie Freiberg High Resolution Geochronological Laboratory University of S?o Paulo

Compositionally zoned plutons, both layered and concentrically arranged, provide granitic exposures where the mechanisms and timing of the magmatic emplacement processes can be studied. The importance of in-situ geochemical differentiation and the magma replenishment rates are revealed by geochemistry and field relations, together with the increasingly accurate U-Pb geochronology, which has promoted the knowledge about the pluton incremental assembly theories.The Flamenco pluton, located in the Coastal Range of northern Chile, is part of the Upper Triassic to Early Cretaceous Andean intrusives formed in the western active margin of South America, and present a normal zoned structure with mafic magmatic facies(mostly gabbros and Qtz-diorites) close to the contacts with the host metasediments, and tonalites, granodiorites and granites in the inner areas. A combined study of the field relations, geochemistry and zircon geochronology of the magmatic facies was applied to determine the emplacement sequence of the Flamenco pluton, revealing three distinguishable domains separated by metasedimentary septa. The SW area is constituted by mostly homogeneous leucocratic granodiorites that yielded an age of 213 Ma as the best estimation for their emplacement age. Distinctive geochemical characteristics, such as the absence of an Eu anomaly, the depletion in HREE, or the highest Sr, Sr/Y and Ce/Yb values among the granodioritic facies of the pluton,involve lower T and/or higher P conditions at the magmatic source according to experimental studies.These conditions were established during an early stage of the Andean magmatic arc building that is firstly defined here as Upper Triassic. The NW and E domains of the pluton were sequentially emplaced between 194 Ma and 186 Ma and both the field relations and the detailed geochronological results suggest that the mafic facies intruded latter in the emplacement sequence. To the NW, Qtz-dioritic and gabbroic externally emplaced pulses gave a younger crystallization age of 186.3 ± 1.8

关键词： Zircon U-Pb geochronology Zoned plutons Andean magmatic arc Sequential emplacement Individual and statistical zircon AGEs

Precambrian Captured Zircon AGEs in the Daheigou Formation, Xing’an Block

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Acta Geologica Sinica(English Edition) 2019年第3期93卷 758-759页

作者： SONG Wanbing CUI Fanghua YANG Hongzhi ZHANG Li WU Xinwei SHI Jianmin ZHANG Chao Shenyang Institute of Geology and Mineral Resources China Geological Survey Shenyang 110034 Liaoning China Resources and Environmental Engineering of Shandong University of Technology Zibo 255049 Shandong China

The Xing'an Block (XB) is located between the ErgunaBlock (EB) and the Songnen Block (SB), whichtopographically occupies the northeastern half of the GreatXing'an Range (Liu et al., 2017). The XB wastraditionally considered to be composed of a Precambrianmetamorphic basement and post-Cambrian cover.However, the recent geochronological studies show thatthe so-called "Precambrian metamorphic rocks^^ are muchyounger in age and considered to be formed in the EarlyPaleozoic, indicating no existence of the Precambrianmetamorphic basement.

关键词： Xing'an Block Precambrian Captured Zircon AGEs

MRTF-A通过NF-κB参与AGEs诱导肾小球系膜细胞中FN、ICAM-1的表达

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中国药理学通报 2017年第4期33卷 572-577页

作者：陈秋红陈志泉黄佳妮黄河清中山大学药学院广东省疾病模式动物工程技术研究中心广东广州510006

目的该研究观察在糖基化终末产物(AGEs)诱导大鼠肾小球系膜细胞(GMCs)中,心肌素相关转录因子MRTFA的变化及其对细胞黏附分子(ICAM-1)及纤维连接蛋白(FN)的影响,探讨MRTF-A是否通过影响NF-κB信号通路参与糖尿病肾病。方法 AGEs条件下,... 详细信息

目的该研究观察在糖基化终末产物(AGEs)诱导大鼠肾小球系膜细胞(GMCs)中,心肌素相关转录因子MRTFA的变化及其对细胞黏附分子(ICAM-1)及纤维连接蛋白(FN)的影响,探讨MRTF-A是否通过影响NF-κB信号通路参与糖尿病肾病。方法 AGEs条件下,抑制剂CCG-1423抑制MRTF-A,或对MRTF-A进行过表达,干扰处理,通过Western blot检测MRTF-A、ICAM-1和FN的蛋白水平变化及p65在细胞核内的水平。结果在AGEs诱导的GMCs中,MRTF-A的蛋白表达增加;干扰或抑制MRTF-A能够下调AGEs诱导的FN和ICAM-1的蛋白表达及p65入核水平;而过表达MRTF-A则进一步上调FN和ICAM-1的蛋白表达及p65入核水平。结论 AGEs能够上调GMCs中MRTF-A的表达,且MRTF-A通过影响NF-κB信号通路参与介导了AGEs诱导的FN和ICAM-1的蛋白表达。

关键词：糖尿病肾病肾小球系膜细胞 AGEs MRTF-A p65 FN ICAM-1

梓醇、马钱苷及其配伍干预AGEs-RAGE通路对糖尿病睾丸病变的保护作用研究

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中国药理学通报 2020年第7期36卷 998-1005页

作者：陈玉萍焦妮朱逸辉丁网丽刘梦雪李新亮许惠琴江苏医药职业学院基础医学部江苏盐城224005 南京大学金陵学院化学与生命科学学院江苏南京210089 江苏省中医院药学部 210029 南京中医药大学药学院江苏南京210023

目的研究梓醇、马钱苷及其配伍对糖尿病睾丸病变的保护作用及机制。方法采用KK-Ay自发性糖尿病小鼠模型和糖基化终末产物诱导小鼠精母细胞株(GC-2)损伤模型,分组干预,HE染色检测睾丸的病理改变;试剂盒法检测睾丸特异性标志酶、SOD活性和... 详细信息

目的研究梓醇、马钱苷及其配伍对糖尿病睾丸病变的保护作用及机制。方法采用KK-Ay自发性糖尿病小鼠模型和糖基化终末产物诱导小鼠精母细胞株(GC-2)损伤模型,分组干预,HE染色检测睾丸的病理改变;试剂盒法检测睾丸特异性标志酶、SOD活性和ATP、ROS水平;AO/EB染色法检测GC-2的凋亡;Western blot法测定AGEs-RAGE通路相关蛋白的表达。结果梓醇、马钱苷及其配伍均可改善糖尿病小鼠睾丸病理损伤,降低血糖,升高睾丸特异性标志酶活力,降低AGEs诱导的GC-2细胞凋亡率,改善氧化应激,配伍组改善效果较优,同时梓醇、马钱苷及其配伍可下调模型组睾丸组织、GC-2细胞的RAGE及其下游蛋白Nox4的表达、抑制p38MAPK磷酸化。结论梓醇、马钱苷及其配伍对糖尿病睾丸病变有明显的保护作用,配伍组药效优于单用组,其作用机制与干预AGEs-RAGE通路相关。

关键词： AGEs 马钱苷梓醇配伍 RAGE 糖尿病睾丸病变

真空低热协同茶多酚处理对鲟鱼肉AGEs形成的影响及其调控机制研究

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真空低热协同茶多酚处理对鲟鱼肉AGEs形成的影响及其调控机制研究

作者：韦剑玲浙江工商大学

学位级别：硕士

晚期糖基化终末产物（AGEs）来源于蛋白氨基与还原糖羰基在非酶条件下的相互作用而产生的有害物质。水产品在热加工过程中会产生AGEs,食源性AGEs的积累也会加剧人体糖尿病、肾病等慢性疾病的发生。本文以俄罗斯鲟鱼为研究对象,分析真空... 详细信息

晚期糖基化终末产物（AGEs）来源于蛋白氨基与还原糖羰基在非酶条件下的相互作用而产生的有害物质。水产品在热加工过程中会产生AGEs,食源性AGEs的积累也会加剧人体糖尿病、肾病等慢性疾病的发生。本文以俄罗斯鲟鱼为研究对象,分析真空低热处理（LTVH）技术处理对鲟鱼肉AGEs形成的影响以及茶多酚协同干预对于AGEs的抑制作用机制。（1）与对照组（TC）相比,LTVH处理的鱼肉获得更好的食用品质,但AGEs含量较高。茶多酚的添加可以改善鱼肉的色差,抑制不良风味,并对LTVH鱼肉AGEs的产生有显著的抑制作用;茶多酚对AGEs的抑制效果具有浓度依赖性,低浓度（20μg/m L）和中浓度（200μg/m L）的茶多酚显著抑制LTVH鲟鱼肉的游离羧甲基赖氨酸（CML）、游离羧乙基赖氨酸（CEL）和结合CEL浓度（P＜0.05）,但高浓度（2000μg/m L）的茶多酚会促进游离CML的生成。（2）蛋白组学技术分析了糖基化模型中蛋白的修饰类型和修饰位点,发现高温会加剧糖基化修饰蛋白数量、修饰位点数量和修饰类型数量。高温处理组鉴定的蛋白则高达143种,远高于60℃的处理组鉴定蛋白数（49种）。茶多酚的添加可减少蛋白的糖基化修饰,6015B/G获得最低的糖基化修饰蛋白数量、修饰位点数量,分别为34、70个,其吡咯啉修饰、羧甲基化修饰、羧乙基化修饰数量分别为0、31、10个,均低于未添加茶多酚处理组数量（9、42、12个）。（3）体外模型结果表明蛋白通过侧链氨基与葡萄糖作用形成AGEs。高温会加剧此反应过程,产生更多的Amadori产物和α-二羰基化合物。而茶多酚可葡萄糖竞争性结合鲟鱼肌原纤维蛋白的游离氨基,减少葡萄糖与蛋白的糖基化位点和数量,抑制Amadori产物和α-二羰基化合物的产生,从而抑制AGEs的形成,但茶多酚抑制效果在高温下减弱。（4）利用时间动力学和热力学方法分析了蛋白与茶多酚的相互作用,发现热处理初期蛋白的赖氨酸降低迅速,4 min后基本保持不变（P＞0.05）;而精氨酸耐热性较好,在0～10 min内与茶多酚持续作用。热处理过程中蛋白模拟体系主导作用力由前期的离子键、氢键和疏水作用变成了疏水作用和二硫键,蛋白二级结构也由α-螺旋转变成β-折叠和β-转角。热力学结果表明茶多酚与鲟鱼肌原纤维蛋白的结合有两种结合类型,是一个放热熵增过程,1分子蛋白可与10～11分子茶多酚进行结合。

关键词：鲟鱼肉 AGEs 蛋白组学分析形成及调控机制真空低热处理

Source of the Ore-forming Adakitic Porphyry at the Beiya Super-large Au Deposit, Western Yangtze Craton: New Evidence from Zircon U-Pb AGEs of the Amphibolite Xenoliths

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Acta Geologica Sinica(English Edition) 2020年第1期94卷 208-209页

作者： LIU Siqi ZHENG Yuanchuan SHEN Yang HOU Zengqian WANG Lu WANG Zixuan State Key Laboratory of Geological Processes and Mineral Resources and School of Earth Science and ResourcesChina University of GeosciencesBeijing 100083China Institute of Geology Chinese Academy of Geological SciencesBeijing 100037China

Objective The Beiya super-large Au-rich porphyry deposit(304 t Au,2.4 g/t Au)is located within the western Yangtze craton,to the southeast of the Sanjiang Tethyan Orogen(Fig.1).The ore-forming porphyry is adakitic,characterized by high Sr/Y and La/Yb ratios coupled with low Y and Yb contents,and is generally thought to be derived from partial melting of thickened mafic lower crust.The lower crust underneath the western Yangtze craton is mainly composed of ancient crust with Archean AGEs,juvenile crust resulting from the Neoproterozoic subduction(740–1000 Ma),and late Permian juvenile crust related to the Emeishan mantle plume.Which lower crustal end-member has played a critical role in genesis of the Beiya ore-forming porphyry can be constrained by zircon U-Pb AGEs of amphibolite xenoliths hosted in the ore-forming porphyry,because these xenoliths represent direct samples of the source.In this study,we present new zircon U-Pb AGEs of these amphibolite xenoliths to have insight into the nature of the Beiya adakitic porphyry source.

关键词： Western Yangtze Craton New Evidence from Zircon U-Pb AGEs of the Amphibolite Xenoliths Source of the Ore-forming Adakitic Porphyry at the Beiya Super-large Au Deposit