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两种茶提取物对牛蛙心脏和骨骼肌影响的比较及机制研究

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西南农业学报 2021年第10期34卷 2301-2306页

作者：沙爱龙赵雪雯何璐汐黄燕婕重庆三峡学院教师教育学院重庆万州404120 重庆三峡学院生物与食品工程学院/重庆市渝东北特色生物资源开发利用工程技术研究中心重庆万州404120

【目的】研究普洱茶提取物和苦丁茶提取物对牛蛙心脏和骨骼肌的影响及其作用机制。【方法】将牛蛙腓肠肌分别浸泡在两种茶提取物中,记录收缩各指标。测定两种茶提取物对坐骨神经-腓肠肌接头处乙酰胆碱(ACh)含量和乙酰胆碱酯酶(A-CHE)活... 详细信息

【目的】研究普洱茶提取物和苦丁茶提取物对牛蛙心脏和骨骼肌的影响及其作用机制。【方法】将牛蛙腓肠肌分别浸泡在两种茶提取物中,记录收缩各指标。测定两种茶提取物对坐骨神经-腓肠肌接头处乙酰胆碱(ACh)含量和乙酰胆碱酯酶(A-CHE)活性的影响;分别用不同浓度的两种茶提取物灌流离体蛙心,记录收缩变化;分别选取CaCl_(2)、ACh与高浓度茶提取物共同孵育,观察对蛙心收缩的影响。【结果】两种茶提取物均对骨骼肌收缩舒张具有促进作用,作用机制与通过促进终池内Ca^(2+)进入肌浆,使肌浆内Ca^(2+)浓度升高和通过抑制接头处A-CHE活性,使ACh含量增加有关;两种茶提取物均能抑制离体蛙心收缩,均能协同ACh对离体蛙心收缩的抑制作用、抑制CaCl_(2)对其促进作用,作用机制可能与阻断I_(Ca-L)和激活心肌膜M受体,使胞内cAMP浓度减少抑制胞外Ca^(2+)内流有关。【结论】无论对骨骼肌还是心肌,都是普洱茶较苦丁茶影响更明显。

关键词：苦丁茶普洱茶牛蛙在体骨骼肌离体蛙心 Ca^(2+) ACh

慢性间歇性低压低氧对发育大鼠主动脉舒张的影响

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中国药理学通报 2020年第2期36卷 215-220页

作者：李宏伟宋丽莉张翼董泽飞邢台医学高等专科学校生理教研室河北邢台054000 河北医科大学生理教研室河北石家庄050017

目的探讨慢性间歇性低压低氧(chronic intermittent hypobaric hypoxia,CIHH)对发育大鼠胸主动脉舒张功能的影响及作用机制。方法新生♂Sprague-Dawlay大鼠,随机分为8组:CIHH处理(CIHH)、CIHH后1周(CIHH-p1)、CIHH后2周(CIHH-p2)、CIHH后3周(CIHH-p3)、对照(Con)、1周对照(Con-1)、2周对照(Con-2)与3周对照(Con-3)。CIHH大鼠给予42 d、每天5 h、模拟海拔3 km的低压低氧处理。制备胸主动脉环,描记舒张活动。结果CIHH明显增强动脉环内皮依赖性舒张(P<0.05),此效应至少可维持3周时间(P<0.05);CIHH明显减弱急性低氧对动脉环舒张的抑制(P<0.05);CIHH对胸主动脉环舒张的增强效应及抗低氧作用可被前列环素抑制剂吲哚美辛、K ATP通道阻断剂格列本脲和氧自由基清除剂Tempo所阻断。结论CIHH处理增强发育大鼠主动脉内皮依赖性舒张,并对抗急性低氧对舒张的抑制,此作用与K ATP通道开放、前列环素和氧自由基作用有关。

关键词：慢性间歇性低压低氧急性低氧胸主动脉环 ACh 前列环素 K ATP通道氧自由基发育大鼠

Dynamic Expression of Hyperpolarization-activated Cyclic Nucleotide-gated Cation Channel 4 Involved in Microwave Induced Pacemaker Cell Injuries

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Biomedical and Environmental Sciences 2015年第11期28卷 823-828页

作者： LIU Yan Qing ZHAO Li GAO Ya Bing DONG Ji WANG Hui YAO Bin Wei ZHOU Hong Mei WANG Shui Ming PENG Rui Yun Department of Experimental Pathology Beijing Institute of Radiation Medicine The Affiliated Hospital of Logistic College of Chinese People’s Armed Police Forces Department of Radiation Protection Beijing Institute of Radiation Medicine

To investigate the mechanisms of microwave induced pacemaker cell injuries, Wistar rats and the primary pacemaker cells of newborn Wistar rats were exposed to microwave at average power density of 50 mW/cm2. Slower spontaneous beating rate, intercellular Ca2＋ aggregation and cell membrane perforation were detected immediately after the exposure. Moreover, hyperpolarizationactivated cyclic nucleotide-gated cation channel 4 （HCN4） was down-regulated immediately after the exposure and up-regulated at 12 h after the exposure. In the sinoatrial node （SAN） of the rats,

关键词： HCN In node Dynamic Expression of Hyperpolarization-activated Cyclic Nucleotide-gated Cation Channel 4 Involved in Microwave Induced Pacemaker Cell Injuries SAN ISH Figure ACh AR

香参丸对结肠炎大鼠结肠粘膜代谢关键酶的调控作用

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时珍国医国药 2019年第11期30卷 2561-2563页

作者：刘億刘端勇刘雪珂刘馥春张晓云陈芳赵海梅江西中医药大学江西南昌330004 江西省中药药理学重点实验室江西南昌330004 江西中医药大学科技学院江西南昌330004 江西中医药大学生命科学学院江西南昌330004

目的评价香参丸治疗实验性结肠炎大鼠疗效,并从香参丸对结肠炎大鼠结肠黏膜关键酶的调节作用探讨其作用机制。方法将40只SD大鼠随机平均分成正常组、模型组、香参丸组、美沙拉嗪组,给予3mg/kg TNBS/50%乙醇混合溶液灌肠复制模型,灌胃给... 详细信息

目的评价香参丸治疗实验性结肠炎大鼠疗效,并从香参丸对结肠炎大鼠结肠黏膜关键酶的调节作用探讨其作用机制。方法将40只SD大鼠随机平均分成正常组、模型组、香参丸组、美沙拉嗪组,给予3mg/kg TNBS/50%乙醇混合溶液灌肠复制模型,灌胃给药7d。通过测量结肠重量和长度、计算结肠重量指数、观察组织病理切片评价香参丸疗效,检测结肠黏膜ADH、ChAT、NADP^+、ACh和MAO含量。结果与模型组比较,香参丸组结肠长度明显增长,重量和结肠重量指数下降,ADH、ChAT和NADP^+均下降,而ACh和MAO显著升高(PACh的表达量起到治疗实验性大鼠结肠炎作用。

关键词：香参丸结肠炎 ADH ChAT NADP^+ MAO ACh

Acetylcholinesterase inhibitor modifications: a promising strategy to delay the progression of Alzheimer's disease

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Neural Regeneration Research 2015年第1期10卷 43-45页

作者： Soumee BhattACharya Dirk Montag Leibniz Institute for Neurobiology Neurogenetics Special Laboratory

Alzheimer＇s disease （AD）, a fatal, progressive, neurodegener- ative disorder, is the most common cause of old-age demen- tia, accounting for 50-75% of dementia patients. Early stages of AD are marked by vocabulary shrinkage, spatial disori- entation, depression, apraxia, and deterioration of recent forms of declarative memory. In course of time, the patients require close supervision due to the loss of cognitive and functional abilities, and at the terminal stages of the disease, all forms of memory are severely impaired with the patients needing nursing home care （World Alzheimer Report, 2013）.

关键词： Acetylcholinesterase inhibitor modifications a promising strategy to delay the progression of Alzheimer’s disease ACh oral

Targeting α7 nicotinic acetylcholine receptors: a future potential for neuroprotection from traumatic brain injury

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Neural Regeneration Research 2015年第10期10卷 1552-1554页

作者： Samuel S.Shin C.Edward Dixon Department of Neurological Surgery University of Pittsburgh Medica Center

Traumatic brain injury （TBI） poses a significant socioeconomic burden in the world. The long lasting consequences in cognitive impairments are often underreported and its mechanisms are unclear. In this perspective, cholinergic dysfunction and thera-peutic strategy targeting this will be reviewed. Novel agents that can target specific subtype of acetylcholine receptors have been developed over the recent years and are at various stages of development, which include AR-R 17779, GTS-21, SSR- 180711A, AR-R17779, and PNU-282987. A detailed review on this topic has been previously published （Shin and Dixon, 2015）.

关键词： TBI nicotinic acetylcholine receptors Targeting a future potential for neuroprotection from traumatic brain injury ACh

趋化因子CCL2对大鼠学习记忆的影响及其机制

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中国药理学通报 2019年第7期35卷 962-967页

作者：陈健民谭丽秋蒋俊俊梁冰玉周燕广西医科大学药学院广西南宁530021 广西医科大学广西艾滋病防治研究重点实验室广西南宁530021

目的研究CC类趋化因子配体2(CCL2)对大鼠学习记忆的影响及机制。方法 40只♂ SD大鼠随机分为5组:空白对照组、假手术组、CCL2(0.5、5、50 ng)组。除空白对照组外,其余各组均进行双侧海马注射。注射后的d3~8进行Morris水迷宫实验,d10分... 详细信息

目的研究CC类趋化因子配体2(CCL2)对大鼠学习记忆的影响及机制。方法 40只♂ SD大鼠随机分为5组:空白对照组、假手术组、CCL2(0.5、5、50 ng)组。除空白对照组外,其余各组均进行双侧海马注射。注射后的d3~8进行Morris水迷宫实验,d10分离各组大鼠海马,qPCR检测凋亡蛋白caspase-8、caspase-3以及磷酸激活谷氨酰胺酶(PAG)mRNA的相对表达水平;ELISA检测肿瘤坏死因子ɑ(TNF-ɑ)、乙酰胆碱酯酶(AChE)的含量,以及谷氨酰胺合成酶(GS)的活力。结果与假手术组相比,各CCL2处理组大鼠逃避潜伏期及游泳路程均明显增长,原平台穿越次数和原平台象限路程百分比均明显减少;各CCL2处理组大鼠海马组织中caspase-8、caspase-3、PAG mRNA相对表达量增加;各CCL2处理组大鼠海马组织内TNF-ɑ、AChE的表达量增加,GS活力下降。结论 CCL2能损伤大鼠的学习记忆力,其机制与参与炎症反应、神经兴奋毒性、导致ACh含量下降以及介导细胞凋亡有关。

关键词： CCL2 学习记忆炎症反应兴奋毒性 ACh 细胞凋亡

DDAH1 promotes neurogenesis and neural repair in cerebral ischemia

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Acta Pharmaceutica Sinica B 2024年第5期14卷 2097-2118页

作者： Gao Q. Ni P. Wang Y. Huo P. Zhang X. Wang S. Xiao F. Li Y. Feng W. Yuan J. Zhang T. Li Q. Fan B. Kan Y. Li Z. Qi Y. Xing J. Yang Z. Cheng H. Gao X. Feng X. Xue M. Liu Y. Luo Y. Lu Z. Zhao Y. Department of Pharmacology School of Basic Medical Sciences Capital Medical University Beijing 100069 China Cerebrovascular Diseases Research Institute and Department of Neurology Xuanwu Hospital of Capital Medical University Beijing 100053 China State Key Laboratory for Structural Chemistry of Unstable and Stable Species Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China College of Life Science University of Chinese Academy of Sciences Beijing 100049 China Department of Laboratory Animal School of Basic Medical Sciences Capital Medical University Beijing 100069 China Department of Physiology and Pathophysiology School of Basic Medical Sciences Capital Medical University Beijing 100069 China

Choline acetyltransferase (ChAT)-positive neurons in neural stem cell (NSC) niches can evoke adult neurogenesis (AN) and restore impaired brain function after injury, such as acute ischemic stroke (AIS). However, the relevant mechanism by which ChAT+ neurons develop in NSC niches is poorly understood. Our RNA-seq analysis revealed that dimethylarginine dimethylaminohydrolase 1 (DDAH1), a hydrolase for asymmetric NG,NG-dimethylarginine (ADMA), regulated genes responsible for the synthesis and transportation of acetylcholine (ACh) (Chat, Slc5a7 and Slc18a3) after stroke insult. The dual-luciferase reporter assay further suggested that DDAH1 controlled the activity of ChAT, possibly through hypoxia-inducible factor 1α (HIF-1α). KC7F2, an inhibitor of HIF-1α, abolished DDAH1-induced ChAT expression and suppressed neurogenesis. As expected, DDAH1 was clinically elevated in the blood of AIS patients and was positively correlated with AIS severity. By comparing the results among Ddah1 general knockout (KO) mice, transgenic (TG) mice and wild-type (WT) mice, we discovered that DDAH1 upregulated the proliferation and neural differentiation of NSCs in the subgranular zone (SGZ) under ischemic insult. As a result, DDAH1 may promote cognitive and motor function recovery against stroke impairment, while these neuroprotective effects are dramatically suppressed by NSC conditional knockout of Ddah1 in mice. © 2024 The Authors

关键词： ACh ADMA ChAT DDAH1 HIF-1α Neural repair Neurogenesis Stroke

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梓醇多效性相关信号通路研究进展

中国药理学通报 2015年第9期31卷 1189-1194页

作者：王静欢邹利万东祝慧凤王园秦蕾西南大学药学院暨中医药学院重庆400716 重庆医科大学附属第一医院急诊医学科重症医学科重庆400016

梓醇是一种环烯醚萜类化合物,具有广泛的药理活性,包括抗脑缺血损伤、抗老年性痴呆、抗炎、抑制毛细血管通透性、止痛、抗肿瘤、缓泻、降血糖、保肝及抗衰老等多种药理作用。该文总结了目前梓醇研究所涉及的信号通路,包括:NF-κB信号通... 详细信息

梓醇是一种环烯醚萜类化合物,具有广泛的药理活性,包括抗脑缺血损伤、抗老年性痴呆、抗炎、抑制毛细血管通透性、止痛、抗肿瘤、缓泻、降血糖、保肝及抗衰老等多种药理作用。该文总结了目前梓醇研究所涉及的信号通路,包括:NF-κB信号通路、PI3K/AKT信号通路、BDNF/Trk B信号通路、JAK2/STAT3血管新生信号通路、MAPK信号通路、TGF-β/Smad信号通路、乙酰胆碱信号通路等,以此加深对梓醇分子信号机制的理解和认识,为梓醇后续深入研究提供参考。

关键词：梓醇信号通路 PI3K/AKT BDNF ACh JAK2/STAT3 MAPK