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Global Analysis of Expression Profiles of Rice Receptor-Like Kinase Genes

Molecular Plant 2012年第1期5卷 143-153页

作者： Lin-Lin Gao Hong-Wei Xue National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and EcologyShanghai Institutes for Biological Science(SIBS)ChineseAcademy of Sciences300 Fenglin Road20032 ShanghaiChina

The receptor-like kinases （RLKs） play critical roles in plant development and response to stress stimuli. By perceiving or sensing the extracellular signals, RLK activates the downstream signaling pathway through phosphorylating the specific targets. Up to now, only a few RLKs have been functionally identified, which are even fewer in rice （Oryza sativa L.）. We here report the systemic analysis of the expression profiles of rice RLK coding genes in different tissues, with the emphasis on seed development and in response to both abiotic stress and plant hormones. The results showed that most rice RLK genes are expressed in two or more tissues, of which the RLCK-RLKs have a higher, while WAK- and SD-RLKs have a lower, expression level in the vegetative tissues than other subfamily members. Interestingly, the constitutively highly expressed RLKs in rice and Arabidopsis are conserved, which is consistent with the previous hypothesis that RLKs existed before the differentiation of monocotyledon and dicotyledon plants. Nearly one-third of the detected rice RLKs are expressed during seed development, and the RLCK-RLK members possess a higher percentage during the endosperm de- velopment, suggesting a novel function of RLCK-RLK members in endosperm development. Further analysis revealed that many RLK genes expressed during seed development are also regulated by abiotic stresses （cold, salt, or drought） or hor- mones, indicating that RLKs may take part in the stress-related signaling pathways such as dehydration of endosperm. These results provide informative insights into the RLK studies and will be helpful to reveal the global regulatory network controlling rice seed development.

关键词： Receptor-like kinase rice profiling seed development stress aba.

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Changes of DHN1 expression and subcellular distribution in A.delicisoa cells under osmotic stress

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Science China(Life Sciences) 2002年第1期45卷 1-9页

作者：邱全胜王泽宙蔡起贵姜荣锡 College of Life Sciences Beijing Normal University Beijing 100875 China

The changes of DHN1 expression and subcellular distribution in A. delicisoa cells under osmotic stress were studied by using GFP as a reporter molecule. Through creating the Xba I and BamH I restriction sites at the ends of dhn1 by PCR, the expression vector for the fusion protein DHN1-mGFP4 was constructed by cloning dhn1 into plasmid pBIN-35SmGFP4. Then the DHN1-mGFP4 expression vector was transformed into A. delicisoa suspension cells by microprojectile bombardment method. Bright green fluorescence of GFP which shows the high-level expression of DHN1-mGFP4 was visualized after culture for 10 h. However, the green fluorescence was only located within the nucleus. By increasing the culture medium osmotic potential, the green fluorescence was visualized in the cytoplasm (mainly around the plasma membranes). The generation of GFP fluorescence in the cytoplasm was also promoted by increasing the medium osmotic potential. Moreover, GFP green fluorescence was abolished by protein synthesis inhibitor dicyclohexylcarbodiimid, indicating that the cytoplasmic DHN1 was newly synthesized under osmotic stress. Furthermore, aba.promoted the presence of green fluorescence in the cytoplasm, and the GFP fluorescence was visualized within a shorter time under a higher osmotic potential.

关键词： A. delicisoa suspension cells, osmotic stress, dehydrin DHN1, green fluorescent protein (GFP), transient expression, subcellular distribution, aba.

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Molecular cloning, sequence analysis and expression studies of a novel GAmyb homologous gene, hvmyb

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Science China(Life Sciences) 1998年第5期41卷 525-529页

作者：凌世昀祁放朱玉贤 Peking University Beijing China

Using a known GAmyb gene as the probe, two fully identical clones were isolated from a barley aleurone cDNA library. Sequence analysis showed that their 5′ termini are highly homologous to the 3′ termini of GAmyb (97%) and their 3′ termini share no significant homology with any myb genes. Therefore, the deduced protein may hold intact putative GAmyb activation domain but lack the normal DNA binding domain. Northern blot reveals that hvmyb expression in barley aleurone layers is strongly up regulated by gibberellin (GA) and down regulated by abscisic acid (aba.. The tissue and developmental stage specificity of hvmyb was also found, which was only expressed in barley aleurone cells and dropped to non detectable level soon after germination.

关键词： barley aleurone GAmyb GA aba.

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