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Aression of TLR_9 in human pulmonary adenocarcinoma cell line a549

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The Chinese-German Journal of Clinical Oncology 2009年第7期8卷 393-396页

作者： Jun Yu Tiecheng Pan Xiang Wei Ligang Liu Min Hu Fang Yuan Jiaduo Li Department of Cardiothoracic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science andTechnology Wuahn 430030 China

Objective:Being considered as a bridge between the innate immunity and acquired immunity,Toll-like receptors(TRLs) are very important innate immunity *** researchs on the innate immunity have focused on the relationship between TLRs and human *** paper investgated the expression and significance of TLR9 in human pulmonary adenocarcinoma cell(a549 cell) and human bronchial epithelial cell(HBE cell).Methods:After culturing a549 cell and HBE cell in vitro,the expression of TLR9 mRNA and protein in both cells were detected by immunocytochemistry,Real-time Quantitative Reverse Transcriptase-Polymerase Chain Reaction(Real-time Quantitative PCR) and Western blot,***:By immunocytochemistry staining,TLR9 was mainly expressed in both cells' cell membrane and endochylema as brown-yellow *** showed that the expressions of TLR9 mRNA and protein in a549 cell were stronger than those in HBE cell(P < 0.01).Conclusion:The results suggest TLR9 might cause the progression of human pulmonary adenocarcinoma,and the mechanism needs to be further investgatied.

关键词： TLR9 a549 cell HBE cell immunocytochemistry Real-time Quantitative PCR western blot

Oleanolic acid-induced apoptosis and its relation with intracellular calcium in human lung adenocarcinoma a549 cells

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Journal of Pharmaceutical Analysis 2010年第2期22卷 116-119页

作者： Asmitanand Thakur Department of Respiratory Medicine

Objective To investigate the effect of oleanolic acid (OA) on apoptosis,correlation between apoptosis and intracellular calcium,and its mechanism in human lung adenocarcinoma cell line a549. Methods Human lung adenocarcinoma a549 cells were incubated in vitro and assigned with OA concentrations of 0,10,20 and 40μg/mL. The apoptosis status of a549 cell line was detected with Annexin V-FITC/PI by flow cytometry (FCM); fluorescence intensity (FI) of a549 cells was assessed and the level of intracellular calcium was calculated at 24 hour of OA intervention. The relation between apoptosis and calcium FI was illustrated by curve fitting. Results FCM showed that 10,20 and 40μg/mL of OA could induce a549 cell apoptosis,which followed a concentration-effect pattern; 24-hour intervention with 20μg/mL and 40μg/mL OA showed increased a549 cell apoptosis,and was significantly different from that with 0μg/mL OA (Pcellular calcium concentration in 10,20 and 40μg/mL OA groups was significantly higher than that in 0μg/mL group after 24 hours’ intervention,and the FI showed a trend of increase with increased OA concentration (Pcellular calcium concentration in a549 cells (r=0.981,Pcells in a concentration-dependent manner. The OA-induced apoptosis is responsible for intracellular calcium overload of the tumor.

关键词： oleanolic acid a549 cell apoptosis intracellular calcium flow cytometry

Effect of hypoxia on migration, invasion and adhesion to endothelium of human pulmonary adenocarcinoma a549 cells

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The Chinese-German Journal of Clinical Oncology 2008年第9期7卷 509-514页

作者： Weigan Shen Jun Zhu Zhiyong Yu Qingyu Xue Department of cell Biology Medical College of Yangzhou University Yangzhou 225001 China Department of Biology Yangzhou Educational College Yangzhou 225000 China

Objective： To evaluate the effect of hypoxia on migration, invasion and adhesion to endothelium of human pulmo- nary adenocarcinoma a549 cells. Methods： Wound-healing and Transwell invasion assays were performed to study the effect of hypoxia on migration and invasion of a549 cells, and a549 cells were added to a monolayer of human umbilical vein endo-thelial cells （HUVECs） to test the ability to adhere to endothelium. Immunofluorescence assay and luciferase reporter gene assay were also used to evaluate the effect of hypoxia on distribution of E-cadherin, β-catenin, and actin, and hypoxia-inducible factor-1 （HIF-1）-dependent transcription, respectively. Results： Hypoxia facilitated a549 cell migration, invasion, and a549 cell-endothelial cells adhesion, and modulated the distribution of E-cadherin and β-catenin, and actin cytoskeleton rearrange-ment, and up-regulated HIF-l-dependent reporter gene expression in a549 cells. Conclusion： Promotion ofa549 cell migration, invasion, and adhesion on endothelium by hypoxia might be modulated through its up-regulating HIF-l-dependent gene expression, which then induced the redistribution of E-cadherin and β-catenin, and the actin cytoskeletal reorganization.

关键词： Hypoxia migration invasion adhesion a549 cell

Carbon quantum dots/Bi_(4)O_(5)Br_(2) photocatalyst with enhanced photodynamic therapy:killing of lung cancer(a549)cells in vitro

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Rare Metals 2022年第1期41卷 132-143页

作者： Bing He Hai-Yan Jin Ya-Wen Wang Cai-Mei Fan Yun-Fang Wang Xiao-Chao Zhang Jian-Xin Liu Rui Li Jue-Wen Liu College of Chemistry and Chemical Engineering Taiyuan University of TechnologyTaiyuan 030024China Department of Chemistry University of WaterlooWaterlooON N2L 3G1Canada

Inorganic photocatalysts have been regarded as a promising candidate in the domain of tumor photodynamic therapy(PDT)due to their inspirational photocatalytic *** this study,a Bi_(4)O_(5)Br_(2)photocatalyst was synthesized and it exhibited effective photo-killing activity of a549 cells(a human lung carcinoma epithelial cell line)in *** this basis,we modified Bi_(4)O_(5)Br_(2)with carbon quantum dots(CQDs)via a hydrolysis method at room temperature,which resulted in an improved photo-killing effect of Bi_(4)O_(5)Br_(2)to a549 *** samples and the interaction between samples and cells were fully *** has been found that the loading of CQDs on Bi_(4)O_(5)Br_(2)can reduce the hydration ratio,increase the cellular uptake and improve the photogenerated reactive oxygen species(ROS)as compared with pristine Bi_(4)O_(5)Br_(2).Electron spin resonance(ESR)analysis and radical-trapping experiments manifested that the ROS contributed to PDT may be·O^(2-)and ·*** study may provide a useful strategy to ameliorate the penetrability,cell compatibility and PDT effect upon cancer cells of other inorganic photocatalysts.

关键词： CQDs/Bi_(4)O_(5)Br_(2) Photocatalyst PDT a549 cell Reactive oxygen species

miR-185/YWHAZ轴通过糖酵解调控NSCLC a549细胞凋亡

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中南医学科学杂志 2023年第3期51卷 330-334页

作者：马建刚王冬谭维周凤张莉媛陕西中医药大学第二附属医院呼吸内科陕西咸阳712000

目的探讨miR-185/YWHAZ轴通过糖酵解对非小细胞肺癌(NSCLC)a549细胞凋亡的影响。方法糖酵解抑制剂处理后,检测各组糖酵解酶、乳酸、NADH水平和细胞凋亡水平。过表达或敲低miR-185后,过表达或敲低YWHAZ后,均检测细胞乳酸、NADH和细胞... 详细信息

目的探讨miR-185/YWHAZ轴通过糖酵解对非小细胞肺癌(NSCLC)a549细胞凋亡的影响。方法糖酵解抑制剂处理后,检测各组糖酵解酶、乳酸、NADH水平和细胞凋亡水平。过表达或敲低miR-185后,过表达或敲低YWHAZ后,均检测细胞乳酸、NADH和细胞凋亡水平。荧光素酶报告实验检测miR-185与YWHAZ mRNA的相互作用。结果糖酵解抑制剂处理后,糖酵解酶、乳酸、NADH下降,细胞凋亡水平上升(P<0.05)。过表达miR-185时,细胞乳酸、NADH下降,YWHAZ mRNA和蛋白水平下降,细胞凋亡水平上升;敲低miR-185时上述变化逆转(P<0.05)。敲低YWHAZ时,细胞乳酸、NADH下降,细胞凋亡水平上升;过表达YWHAZ时上述变化逆转(P<0.05)。miR-185靶向YWHAZ mRNA的3′端非翻译区。结论 YWHAZ、miR-185分别是促进和抑制糖酵解的关键分子。miR-185靶向YWHAZ mRNA的3′端非翻译区,减少了YWHAZ表达,促进了NSCLC的细胞凋亡。

关键词： miR-185 YWHAZ 非小细胞肺癌凋亡糖酵解 a549 cell