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浙江中西医结合杂志 2015年第2期25卷 111-114页

作者：周勇赵玮张君杭州市红十字会医院干部保健科杭州310003 浙江中医药大学附属第一医院肺功能室杭州310006 浙江中医药大学杭州310053

目的观察麻杏二三汤对哮喘小鼠肺组织气道黏蛋白(MUC)5ac m RNA的影响,探讨其抑制哮喘小鼠气道黏液高分泌的作用机制。方法雌性BALB/c小鼠60只,随机分成空白对照组、哮喘模型组、强的松组及麻杏二三汤高、中、低剂量组,每组10只。除空... 详细信息

目的观察麻杏二三汤对哮喘小鼠肺组织气道黏蛋白(MUC)5ac m RNA的影响,探讨其抑制哮喘小鼠气道黏液高分泌的作用机制。方法雌性BALB/c小鼠60只,随机分成空白对照组、哮喘模型组、强的松组及麻杏二三汤高、中、低剂量组,每组10只。除空白对照组,另五组以腹腔注射0.1%卵白蛋白(OVA)与10%氢氧化铝混悬溶液致敏,以1%OVA雾化液雾化攻击制作哮喘小鼠模型。分别给予生理盐水、生理盐水、强的松(10.0mg/kg)、麻杏二三汤高剂量(30g/kg)、中剂量(15g/kg)、低剂量(7.5g/kg)灌胃,R-T PCR测定各组MUC5ac m RNA表达。结果麻杏二三汤高、中、低剂量组MUC5ac m RNA荧光定量△Ct值分别为(12.80±0.84)、(12.15±0.49)、(11.41±1.49),均高于哮喘模型组的(9.82±0.84)(P5ac m RNA表达有关。

关键词：小鼠哮喘 MUC 5ac mRNA 麻杏二三汤

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Somatic molecular changes and histo-pathological features of colorectal cancer in Tunisia

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World Journal of Gastroenterology 2013年第32期19卷 5286-5294页

作者： Sana Aissi Marie Pierre Buisine Farid Zerimech Nadia Kourda Amel Moussa Mohamed Manai Nicole Porchet Laboratory of Biochemistry and Molecular Biology Science University of Tunis2092 El Manar TunisTunisia Centre de Recherche Jean-Pierre Aubert INSERM U83759000 LilleFrance Laboratory of Biochemistry and Molecular Biology CHRU de Lille59000 LilleFrance Medicine University of H Warembourg University of Lille Nord de France59045 LilleFrance Anatomopathology Department Charles Nicolle Hospital of Tunis1006 TunisTunisia Gastro-Enterology Department Charles Nicolle Hospital of Tunis1006 TunisTunisia

AIM:To determine correlations between family history,clinical features and mutational status of genes involved in the progression of colorectal cancer(CRC).METHODS:Histo-pathological features and molecular changes[KRAS,BRAF and CTNNB1 genes mutations,microsatellite instability(MSI)phenotype,expression of mismatch repair(MMR)and mucin(MUC)5ac proteins,mutation and expression analysis of TP53,MLH1promoter hypermethylation analysis]were examined in a series of 51 unselected Tunisian CRC patients,10 of them had a proven or probable hereditary disease,on the track of new tumoral markers for CRC susceptibility in Tunisian ***:As expected,MSI and MMR expression loss were associated to the presence of familial CRC(75%vs 9%,P53(exons 4-9)alterations.A significant inverse relationship has been observed between the presence of MSI and TP53 accumulation(10.0%vs 48.8%,P=0.0335)in CRC tumors,suggesting different molecular pathways to CRC that in turn may reflect different environmental ***,MUC5ac expression was significantly associated to the presence of MSI(46.7%vs 8.3%,P=0.0039),MMR expression loss(46.7%vs8.3%,P=0.0039)and the presence of familial CRC(63%vs 23%,P=0.039).CONCLUSION:These findings suggest that MUC5ac expression analysis may be useful in the screening of Tunisian patients with high risk of CRC.

关键词： DNA mismatch repair KRAS TP53 Mucin 5ac

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Differential mucin phenotypes and their significance in a variation of colorectal carcinoma

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World Journal of Gastroenterology 2013年第25期19卷 3957-3968页

作者： Yasuo Imai Hidetsugu Yamagishi Kazunori Fukuda Yuko Ono Tohru Inoue Yoshihiko Ueda Department of Pathology Dokkyo Medical University Koshigaya Hospital Joint Research Center Dokkyo Medical University Koshigaya Hospital Department of Pathology Tokyo Kosei Nenkin Hospital

AIM: To investigate mucin expression profiles in colorectal carcinoma (CRC) histological subtypes with regard to clinicopathologic variables and prognosis. METHODS: Mucin (MUC)2 and MUC5ac expressions were assessed by immunohistochemistry for a total of 250 CRC cases that underwent surgical resection. CRCs included 63 well-to-moderately differentiated adenocar-cinomas (WMDAs), 91 poorly differentiated adenocarcinomas (PDAs), 81 mucinous adenocarcinoma (MUAs), and 15 signet-ring cell carcinomas (SRCCs). MUC2 and MUC5ac were scored as positive when ≥ 25% and ≥ 1% of cancer cells were stained positive, respectively. The human mutL homolog 1 and human mutS homolog 2 expressions were assessed by immunohistochemistry in PDAs to investigate mismatch-repair (MMR) *** that did not express either of these two were considered MMR-deficient. Results were analyzed for associations with clinicopathologic variables and the prognosis in individual histological CRC subtypes. RESULTS: MUC2-positive and MUC5ac-positive WMDA percentages were 49.2% and 30.2%, respectively. In contrast, MUC2-positive and MUC5ac-positive PDA percentages were 9.5% and 51.6%, respectively. MUC2 levels tended to decrease and MUC5ac levels tended to increase from WMDA to PDA. In 21 tumors comprising both adenoma and adenocarcinoma components in a single tumor (4 WMDAs, 7 PDAs, and 10 MUAs), MUC2 was significantly downregulated in PDA and MUC5ac was downregulated in PDA and MUA in the adenoma-carcinoma sequence. These results suggested that MUC2 levels might be associated with malignant potential and that MUC5ac expression was an early event in tumorigenesis. Despite worse prognoses than WMDA, high MUC2 expression levels were maintained in MUA (95.1%) and SRCC (71.5%), which suggested a pathogenesis for these subtypes distinct from that of WMDA. No significant associations were found between MUC2 expression and any clinicopathologic variables in any histological subtype. MUC5ac expression in PDA was

关键词： Mucin 2 Mucin 5ac Microsatellite instability Mismatch repair Colorectal carcinoma Poorly differentiated adenocarcinoma Pathogenesis Adenoma-carcinoma sequence Prognosis

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