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A novel undifferentiated spermatogonia-speci?c surface protein 1(USSP1) in neonatal mice

Science Bulletin 2019年第8期64卷 524-533页

作者： Zhuoheng Lin Puping Liang Zhaokai Yao Yuxi Chen Xiya Zhang Rui Huang Zhen Zhang Minyan Li Wenbin Ma Haiyan Zheng Shanbo Cao Guang Shi Xiaoyang Zhao Zhou Songyang Junjiu Huang Key Laboratory of Gene Function and Regulation of the Ministry of Education State Key Laboratory of Biocontrol School of Life Sciences Sun Yat-sen University Key Laboratory of Reproductive Medicine of Guangdong Province School of Life Sciences and the First Affiliated Hospital Sun Yat-sen University department of developmental Biology School of Basic Medical Science Southern Medical University Key Laboratory of Reproductive Medicine of Guangdong Province Third Affiliated Hospital of Guangzhou Medical University State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University

Mammalian spermatogenesis is maintained by a rare population of spermatogonial stem cells(SSCs),which are important for male fertility. SSCs remain a subset of undifferentiated spermatogonia, which can be isolated by a combination of surface markers. Specific markers to identify and isolate undifferentiated spermatogonia are lacking. Ussp1, a transcript previously annotated as long noncoding RNA(rikEN cdNA 4933427d06, Gene Id: 232217), virtually encodes a membrane protein, USSP1, in a highly testisspecific manner in mouse. We demonstrate its expression on the membrane of undifferentiated spermatogonia by a homemade polyclonal rabbit antibody against the protein. In vivo, USSP1^+ clusters consist mainly of As, Apr(GFRa1^+) and Aal(PLZF^+) cells. USSP1^+ cells exhibit enrichment of undifferentiated spermatogonia, as shown by increased expression of SSC self-renewal molecular markers and the potential to form SSC clones in vitro and in vivo. However, Ussp1 knockout did not affect the number of SSCs or spermatogenesis in mice. Thy1^+ cells from Ussp1 null mice did not show any defect in the SSC colony formation capacity, indicating that USSP1 is not essential for SSC self-renewal. Our data demonstrate that Ussp1 is specifically expressed in undifferentiated murine spermatogonia, indicating the potential to sort undifferentiated spermatogonia with USSP1 antibodies. Ussp1 might be a good maker for SSC enrichment in neonatal mice.

关键词： 4933427d06rik USSP1 Spermatogenesis Spermatogonial stem cells CRISPR/Cas9

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