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Determining the Minimum Inhibitory Concentration of Bacteriophages: Potential Advantages

Advances in Microbiology 2013年第2期3卷 181-190页

作者： Aradhana Vipra Srividya Narayanamurthy Desai Raghu Patil Junjappa Panchali Roy Nethravathi Poonacha Pallavi Ravinder Bharathi Sriram Sriram Padmanabhan Microbiology Team Gangagen Biotechnologies Pvt. Ltd. Raghavendra layout Opp. MEI Ltd. Bangalore India

The minimum inhibitory concentration (MIC) is the concentration at which an antibacterial agent experiences the complete inhibition of organism growth. Bacteriophages represent a rich and unique resource of anti-infectives to counter the growing world-wide problem of antibiotic resistance. In this study, we compared the host range of lytic bacteriophages and temperate phagesbelonging to various genera, namely Staphylococcus, E. coli and Salmonella, with a range of clinical isolates using two methods: the classical agar overlay method and a newly developed MIC method. MIC was only observed with isolates that were susceptible to phage infection, which correlated with the agar overlay assay, whereas no MIC was detected with isolates that were resistant to phage infection. The simple MIC method was useful in determining phage adsorption and host range, and detecting possible prophage contamination in phage preparations. Interestingly, this method was also applicable to strain differentiation through phage susceptibility testing using a 96-well, high throughput format that proved to be easy, cost-effective, fast and reliable.

关键词： Bacteriophage K MIC Phage 44ahjd Phage P954 Staphylococcus aureus Temperate Phage

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Therapeutic Potential of Staphylococcal Bacteriophages for Nasal Decolonization of <i>Staphylococcus aureus</i>in Mice

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Advances in Microbiology 2013年第1期3卷 52-60页

作者： Madhavi H. Narasimhaiah Jiya Y. Asrani Sundaram M. Palaniswamy Jagadeesh Bhat Shilpa E. George Rajamuthu Srinivasan Aradhana Vipra Srividya N. Desai Raghu Patil Junjappa Panchali Roy Bharathi Sriram Sriram Padmanabhan Gangagen Biotechnologies Pvt. Ltd. Raghavendra Layout Bangalore India

Bacteriophages represent a rich and unique resource of anti-infectives to counter the global problem of antibiotic resis- tance. In this work, we assessed the bactericidal activity of two virulent staphylococcal phages, K and 44ahjd, against S. aureus isolates of clinical significance, and tested their efficacy in vivo. The phage cocktail lysed >85% of the clinical isolates tested. Both the phages were purified by ion-exchange column chromatography following propagation in bioreactors. The purity profiles of the ion-exchange purified phages were compared with those of phages purified using cesium chloride density gradient ultracentrifugation, and infectiousness of the purified phages was confirmed by plaque forming assay. The in vivo efficacy of a phage cocktail was evaluated in an experimental murine nasal colonization model, which showed that the phage cocktail was efficacious. To our knowledge, this is the first report of phage use in an in vivo model of nasal carriage.

关键词： Bacteriophage K Nasal Decolonization Phage 44ahjd Phage Endotoxins Phage Purification Staphylococcus aureus

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