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Carcinogenic potential of duodenal reflux juice from patients with long-standing postgastrectomy

World Journal of Gastroenterology 2001年第3期7卷 376-380页

作者： Zhe Fu Ma~1 Zhong Yu Wang~1 Jun Ran Zhang~2 Peng Gong~1 Hai Long Chen~1 ~1Departrnent of General Surgery,First Hospital,Dalian Medical University,Dalian 116011,China ~2Department of Radiation Oncology,Massachusetts General Hospital and Harvard Medical School,Charlestown,MA 02129,USADr.Zhe Fu Ma graduated from Shanxi Medical College in 1993,nowPh.D.,M.D.in Department of Surgery,Dalian Medical University,majoring gastroenteric cancer,having 4 papers published.Correspondence to:Dr.Zhe Fu Ma.Department of General Surgery,First Hospital,Dalian Medical University,Dalian 116011,China tel.0086-411-4720334 Department of General Surgery First Hospital Dalian Medical University Dalian 116011 China. mazhefu@***

AIM: to determine whether study on the carcinogenic potential of reflux juice from patients with remote gastrectomy could clarify the inherent relationship between duodenal reflux and gastric stump cancer. MEtHODS: A total of 37 reflux juice samples (13 Billroth I, 24 Billroth II) were employed in the present study. A two-stage transformation assay using BALB/c 3t3 cells was carried out to test the initiating or promoting activity of these samples. RESULtS: two of 18 (11.1%) reflux samples exerted initiating activities, whereas 9/19 (47.4%) samples enhanced the MNNG-initiating cell transformation, suggesting the duodenal reflux juice might more frequently possess the tumor-promoter activity (P = 0.029). In addition, there was no difference in initiating activities of the samples irrespective of surgical procedures (P = 0.488), while Billroth II samples exhibited stronger tumor-promoter activity than Billroth I samples (P = 0.027). Furthermore, the promoter activities were well correlated with the histological changes of the stomas (r(s) = 0.625, P = 0.004), but neither their cytotoxicities nor initiating activities had this correlation (Probabilities were 0.523 and 0.085, respectively). CONCLUSION: the duodenal reflux juice from patients with remote postgastrectomy did have carcinogenic potential, and suggested that tumor-promoting activity should principally account for the high incidence of gastric cancer in gastrectomy patients. In contrast, it is difficult to explain the high stump-cancer incidence with the N-nitroso compounds theory-a popular theory for the intact stomach carcinogenesis, and it seemed to be justified to focus chemo-prevention of this cancer on the tumor-promoting potential of reflux juice.

关键词： Gastrectomy Gastric Juice 3t3 cells Adult Aged Animals Carcinogens Cell transformation, Neoplastic Duodenogastric Reflux Female Gastric Stump Gastritis Humans Male Mice Mice, Inbred BALB C Middle Aged Stomach Neoplasms

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Killing effect of tNF-related apoptosis inducing ligand regulated by tetracycline on gastric cancer cell line NCI-N87

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World Journal of Gastroenterology 2001年第4期7卷 559-562页

作者： Xiao-Chao Wei Xin-Juan Wang Kai-Chen Lei Zhang Yu Liang Xin-Li Lin Department of Biochemistry and Molecular Biology,Peking University Health Science Center,Beijing 100083,ChinaProtein Studies,Oklahoma Medical Research Foundation,Oklahoma City,OK 73104,USA Department of Biochemistry and Molecular Biology Peking University Health Science Center 38 Xue Yuan Road Haidian District Beijing 100083 China. xinjuan@***

AIM: to clone the cDNA fragment of human tRAIL (tNF-related apoptosis inducing ligand) into a tetracycline-regulated gene expression system, the Revtet-On system, transduce expression vectors into a gastric carcinoma cell line-NCI-N87 and examine the effects of controlled expression of tRAIL in vitro on the gastric carcinoma cells. MEtHODS: the full-length cDNA of tRAIL was inserted into a vector under the control of the tetracycline-responsive element (tRE) to obtain the plasmid pRevtRE-tRAIL, which was transfected into a packaging cell line Pt67. In addition, vector pRev-tet On and pRevtRE were also transfected into Pt67 separately. After hygromycin and G418 selection, the viral titer was determined. the medium containing retroviral vectors was collected and used to transduce a gastric carcinoma cell line NCI-N87. the resulting cell line NCI-N87-tet On tRE-tRAIL and a control cell line, NCI-N87 tet On-tRE, were established. tRAIL expression in the cell line was induced by incubating cells with doxycycline (Dox), which is a tetracycline analogue. the killing effect on gastric carcinoma cells was analyzed after induction. RESULtS: the recombinant plasmid pRev-tRE-tRAIL was constructed. After hygromycin or G418 selection, the producer cell lines Pt67-tRE, Pt67-tRE-tRAIL and Pt67-tet On were obtained,with titers of about 10(8)CFU.L(-1). By transducing NCI-N87 cells with retroviral vectors from these cell lines, stable cell lines NCI-N87-tet-On tRE-tRAIL (NN3t) and control cell line NCI-N87-tet-On-tRE (NN2t) were established. the growth curves of the selected cell lines were the same with the wild type NCI-N87. When Dox was added, cell death was obvious in the test groups (29%-77%), whereas no difference was observed in control and wild type cell lines. With the addition of a medium from the test group, human leukemia cell line Jurkat was activated till death (83%), indicating the secretion of active tRAIL proteins from the test cells to the medium. CONCLUSION: With th

关键词： Stomach Neoplasms 3t3 cells Animals Anti-Bacterial Agents Apoptosis Apoptosis Regulatory Proteins Doxycycline Gene Expression Regulation, Neoplastic Genetic Vectors Humans Jurkat cells Membrane Glycoproteins Mice Research Support, Non-U.S. Gov't Retroviridae transfection tumor Necrosis Factor-alpha

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