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Study the lipidoid nanoparticle mediated genome editing protein delivery using 3d intestinal tissue model

Bioactive Materials 2021年第11期6卷 3671-3677页

作者： Tao Yang Haobo Han Ying Chen Liu Yang Rachael Parker Yamin Li david L.Kaplan Qiaobing Xu department of Biomedical Engineering Tufts UniversityMedfordMAUSA department of Prosthodontics Guanghua School of StomatologyHospital of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhou510055China Key Laboratory for Molecular Enzymology1nd Engineering of Ministry of Education School of Life SciencesJilin UniversityChangchun130012China

Lipid nanoparticles are promising carriers for oral drug *** bioactive cargos with intracellular targets,***-editing proteins,it is essential for the cargo and carrier to remain complexed after crossing the epithelial layer of intestine in order for the delivery system to transport the cargos inside targeted ***,limited studies have been conducted to verify the integrity of cargo/carrier nanocomplexes and their capability in facilitating cargo delivery intracellularly after the nanocomplex crossing the epithelial ***,we used a traditional 2d transwell system and a recently developed 3d tissue engineered intestine model and demonstrated the synthetic lipid nanoparticle(carrier)and protein(cargo)nanocomplexes are able to cross the epithelial layer and deliver the protein cargo inside the underneath *** found that the EC16-63 LNP efficiently encapsulated the GFP-Cre recombinase,penetrated the intestinal monolayer cells in both the 2d cell culture and 3d tissue models through temporarily interrupting the tight junctions between epithelial *** transporting across the intestinal epithelia,the EC16-63 and GFP-Cre recombinase nanocomplexes can enter the underneath cells to induce gene *** results suggest that the in vitro 3d intestinal tissue model is useful for identifying effective lipid nanoparticles for potential oral drug delivery.

关键词： Lipidoid nanoparticle Protein delivery Oral drug delivery Genome engineering 3d tissue model

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Synthesis of easily-processable collagen bio-inks using ionic liquid for 3d bioprinted liver tissue models with branched vascular networks

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Science China Chemistry 2023年第5期66卷 1489-1499页

作者： Zhiqiang Gao Xin Liu Hao Zhao Shengpeng Xia Wenli Liu Haotian Bai Fengting Lv Xiongfei Zheng Yiming Huang Qi Gu Shu Wang Beijing National Laboratory for Molecular Sciences Key Laboratory of Organic SolidsInstitute of ChemistryChinese Academy of SciencesBeijing 100190China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China College of Chemistry University of Chinese Academy of SciencesBeijing 100049China Shenyang Institute of Automation Chinese Academy of SciencesShenyang 110169China

Bioprinting has been a flouring way to fabricate complex tissue and organ mimics via precisely depositing printable cell-laden ***,there is a limited number of biomaterials that fulfill the mechanical property of printing while meeting the responsive environment desired for the *** excellent cell compatibility and bioactivity,collagen suffers from difficulties in processing and printability which inhibited its utilization in three-dimensional(3d)***,we address this limitation by using ionic liquid as the solvent in the modification process,enabling collagens modified with quantified norbornene for chemical crosslink and extrusion-based 3d *** improved solubility and rheological properties,norbornene-functionalized collagen(Col-Nor)exhibited better shape fidelity in extrusion-based 3d printing compared with the one before ***-crosslinked Col-Nor hydrogel provided structural support and promoted the adhesion,proliferation,and differentiation of various types of cells,which afforded a centimeter-scale liver tissue *** highly generalizable methodology expands printable,versatile,and tunable hydrogels developed from the natural extracellular matrix,allowing the biofabrication of 3d liver tissue model with branched vascular networks.

关键词： bioprinting functionalized collagen rheological properties photo-crosslinking 3d tissue model

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