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Wavelength-dependent three-dimensional single-molecule superlocalization imaging for yoctomole detection of thyroid-stimulating hormone on a quantum dot nanobiosensor

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Chinese Chemical Letters 2023年第12期34卷 518-521页

作者： Junghwa Lee Seungah Lee Seong Ho Kang department of Chemistry Graduate SchoolKyung Hee UniversityYongin-siGyeonggi-do 17104Republic of Korea department of Applied Chemistry and Institute of Natural Sciences Kyung Hee UniversityYongin-siGyonggi-do 14Republic of Kore

A wavelength-dependent three-dimensional(3d)superlocalization imaging method on gold nanoislands(GNIs)chip was developed as a supersensitive single-molecule thyroid-stimulating hormone(TSH)*** and fluorescent signals from gold nanoislands on the substrate and quantum dots(Qds)nanoprobes were simultaneously isolated and acquired within an evanescent field layer generated by total internal reflection(TIR)of incident light using a dual-view *** 3d TIR fluorescence images of TSH-bound Qds on the GNIs were obtained using z-axis optical sectioning at 10nm intervals before/after immunoreaction to identify the optimal conditions for *** localized centroid position of Qd nanoprobes and GNI were distinguished at a subdiffraction limit resolution using 3d Gaussian fitting to the point spread *** Qd TSH nanobiosensor using wavelength-dependent 3d TIR fluorescence-based single-molecule localization microscopy(3d TIRF-SLM)imaging technique showed an excellent detection limit of 90 yoctomoles(~54 molecules)and a wide linear dynamic range of 1.14 zmol/L-100 pmol/L for *** detection sensitivity was about 4.4×10^(9)times higher than conventional enzyme-linked immunosorbent assay and could successfully quantify TSH in human *** wavelength-dependent 3d TIRF-SLM technique may emerge as a reliable platform for ultrahigh-sensitive nanobiosensors at the single-molecule level and early diagnosis with quantification of disease-related ultra-tracebiomolecules.

关键词： Single-molecule nanobiosensor Thyroid stimulating hormone Yoctomole detection 3d superlocalization Imaging

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