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Rice science 2018年第6期25卷 320-329页

作者： WANG Feijuan ZHANG Yiting GUO Qinxin TAN Haifeng HAN Jiahui LIN Haoran WEI Hewen XU Guangwei ZHU Cheng Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province College of Life SciencesChina Jiliang UniversityHangzhou 310018China Jinhua Inspection and Testing Institute of Food and Drug Control Jinhua 321000China

High grain-Cd-accumulating rice variety Yongyou 9 was planted in Cd-contaminated farmland in Taizhou City, Zhejiang Province, China to study the effects of 5-aminolevulinic acid(ALA) and24-epibrassinolide(EBR) on Cd accumulation in brown rice. Results showed that the exogenous ALA and EBR had no significant effects on agronomic traits, soil pH and total Cd content in soil, but had some effects on the available Cd content in soil, and significantly influenced the Cd accumulation in the different parts of rice. Results also showed that 100 mg/L exogenous ALA significantly reduced the Cd accumulation in brown rice to blow the food safety standard(0.2 mg/kg), and also significantly reduced the Cd contents in the roots and culm of rice. However, 200 mg/L exogenous ALA treatment increased the Cd content in brown rice remarkably. In addition, 0.15 mg/L EBR treatment increased Cd accumulation in roots, culm, leaves and brown rice notably, whereas 0.30 mg/L exogenous EBR treatment reduced the Cd accumulation in brown rice properly, but it was not significant. Therefore,proper concentration of ALA can effectively reduce the Cd accumulation in brown rice, which can be used as an effective technical method for the safe production of rice in Cd polluted farmland.

关键词： rice Cd-contaminated soil 5-aminolevulinic acid 24-epibrassinolide Cd accumulation

Effects of 24-epibrassinolide on Antioxidant System in Cucumber Seedling Roots Under Hypoxia Stress

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Agricultural Sciences in China 2007年第3期6卷 281-289页

作者： KANG Yun-yan GUO Shi-rong LI Juan DUAN Jiu-ju College of Horticulture Nanjing Agricultural University Nanjing 210095 P.R.China

This article aims to study the effects of exogenous 24-epibrassinolide （EBR） on the changes in ROS, activities of antioxidative enzymes and antioxidants in cucumber （Cucumis sativus L.） seedling roots under hypoxia stress. Seedlings of a hypoxiaresistant cultivar, Lühachun 4, and a hypoxia-sensitive cultivar, Zhongnong 8, were hydroponically grown for 8 d in normoxic or hypoxic nutrient solutions that were added or not added with 10^-3 mg L^-1 EBR. Under hypoxia stress, the ROS levels and the lipid peroxidation were significantly increased in the roots upon exposure to hypoxia stress, which were inhibited by EBR application. The EBR treatment significantly increased the seedlings growth and SOD, APX, GR activities, and contents of AsA and GSH under hypoxia stress. From the results obtained in this study, it can be concluded that oxidative damage on seedling roots by hypoxia stress can be considerably alleviated and the tolerance of plants was elevated.

关键词： cucumber (Cucumis sativus L.) 24-epibrassinolide seedlings hypoxia stress antioxidative system lipid peroxidation

Amelioration of Aluminium Toxicity in Pigeon Pea [Cajanus cajan (L.) Millsp.] Plant by 24-epibrassinolide

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American Journal of Plant Sciences 2016年第12期7卷 1618-1628页

作者： N. Divya Sri M. Madhan Mohan K. Mahesh K. Raghu S. Seeta Ram Rao Department of Botany Osmania University Hyderabad India

The effect of 24-epibrassinolide on growth of pigeon pea [Cajanus cajan (L.) Millsp.] under aluminium toxicity was studied. 24-EBL reduced the impact of Al stress on plant growth. Particularly 24-EBL reduced the inhibitory impact of aluminium toxicity on root growth which was further manifested in overall improvement of vegetative growth. Application of 24-epibrassinolide removed the inhibitory influence of Al nodulation. The growth stimulation in Cajanus plants by 24-EBL under Al stress was associated with elevated levels of chlorophylls, nucleic acids and soluble proteins. 24-epibrassinolide application enhanced proline content in Al3+ stressed Cajanus plants. Further, the supplementation of 24-epibrassinolide to Al stress treatments increased the activities of antioxidative enzymes viz., catalase [EC 1.***.1.6];peroxidase [EC 1.***.1.7];superoxide dismutase [EC 1.***.1.1] and ascorbate peroxidase [EC 1.***.1.11]. Lipid peroxidation induced by Al was found reduced with the supplementation of 24-epibrassinolide. The present studies demonstrated the ameliorating capability of 24-epibrassinolide on the Al induced inhibition of plant growth of C. cajan.

关键词： Aluminium Toxicity 24-epibrassinolide Cajanus cajan Antioxidative Enzymes

Effect of 24-epibrassinolide on Growth of in VitroShoot Tips of Different Yam (DioscoreaSpp.) Species

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American Journal of Plant Sciences 2013年第11期4卷 2271-2274页

作者： Isabelle Engelmann-Sylvestre Florent Engelmann IRD UMR DIADE Agropolis Montpellier France

In this work we compared the effect of the growth regulator content of the culture medium on the growth of in vitro shoot tips of five yam accessions belonging to four yam species (one Dioscorea alata, one D. rotundata, one D. cayenensis and two D. trifida). Medium S contained 0.6 μM benzyl adenine, 1.07 μM naphthalene acetic acid and 0.23 μM gibberellic acid while medium EBR contained 0.23 μM gibberellic acid and 0.1 μM 24-epibrassinolide. After 2 months of culture, oxidation level was significantly reduced on medium EBR compared to medium S for four of the five accessions tested. By contrast, medium EBR did not have any positive effect on shoot length since length of shoots produced after 2 months of culture on medium S and EBR were similar, except with accession 3-45T, for which shoot length was shorter on medium S compared to medium EBR. These results underline the potential of 24-epibrassinolide to reduce oxidation phenomena during in vitro culture and call for its utilization for regeneration of cryopreserved yam shoot tips, which is often impeded by oxidation phenomena.

关键词： Yam Dioscorea Spp. 24-epibrassinolide Oxidation Shoot Tip In Vitro Culture Cryopreservation

Zinc oxide nanoparticles and epibrassinolide enhanced growth of tomato via modulating antioxidant activity and photosynthetic performance

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BIOCELL 2021年第4期45卷 1081-1093页

作者： MOHAMMAD FAIZAN AHMAD FARAZ SHAMSUL HAYAT JAVAID ABHAT FANGYUAN YU Collaborative Innovation Center of Sustainable Forestry in Southern China College of Forest ScienceNanjing Forestry UniversityNanjing210037China School of Life Sciences Glocal UniversitySaharanpur247121India Plant Physiology Laboratory Department of BotanyFaculty of Life SciencesAligarh Muslim UniversityAligarh202002India State Key Laboratory for Crop Genetics and Germplasm Enhancement College of AgricultureNanjing Agricultural UniversityNanjing210095China

Nanotechnology has greatly expanded the applications of nanoparticles(NPs)domain in the scientific *** this context,the zinc oxide nanoparticles(ZnO-NPs)and 24-epibrassinolide(EBL)has been revealed to positively regulate plant metabolism and *** the present study,we investigated the role of ZnO-NPs and EBL in the regulation of plant growth,photosynthetic efficiency,enzymes activities and fruit yield in *** treatment of ZnO-NPs at three levels(10,50 or 100 ppm)and EBL(10^(−8) M)were applied separately or in combination to the foliage of plant at 35-39 days after sowing(DAS);and the control plants were treated with double distilled water(DDW)only at the same time *** different tested concentrations of ZnO-NPs and/or EBL,the combined spray of 50 ppm of ZnO-NPs and 10^(−8) M of EBL proved to be best,and considerably increased the growth,photosynthetic efficiency,biochemical enzymes activities as well as fruit ***,the performance of the antioxidant enzymes viz catalase,peroxidase and superoxide dismutase were also increased after the combined application of ZnO-NPs and EBL in Lycopersicon ***,it is suggested that combined application of 50 ppm of ZnO-NPs and 10−8 M of EBL is the best combination can be applied to increase the performance and yield of ***.

关键词： Antioxidant enzymes 24-epibrassinolide Lycopersicon esculentum Zinc oxide nanoparticles

Effect of Brassinosteroids on Germination and Seedling Growth of Radish (Raphanus sativusL.) under PEG-6000 Induced Water Stress

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American Journal of Plant Sciences 2013年第12期4卷 2305-2313页

作者： Kommavarapu Mahesh Parshavaneni Balaraju Bellamkonda Ramakrishna Sadhu Seeta Ram Rao Osmania University Hyderabad India

Effect of 24-epibrassinolide and 28-homobrassinolide on the germination and seedling growth of radish (Raphanus sativus) subjected to water stress being imposed by 15% (w/v) polyethylene glycol (equivalent to -2.95 bars of osmotic pressure) was studied. Brassinosteroids supplementation reduced the inhibitory effect of water stress on seed germination and seedling growth. The growth stimulation in radish seedlings by brassinosteroids under desiccation stress was associated with elevated levels of nucleic acids and soluble proteins and lowered activities of RNase. The mitigation of osmotic stress imposed by PEG was associated with increased scavenging of reactive oxygen species as reflected in elevated activities of superoxide dismutase, catalase and ascorbate peroxidase. Brassinosteroids also enhanced the accumulation of the osmolyte free proline in radish seedlings challenged with drought stress. The two brassinosteroids also maintained membrane integrity under water deficiency conditions as indicated by lowered lipid peroxidation reflecting in reduced MDA content. The results of present study demonstrate the protective role of brassinosteroids against PEG imposed water stress in radish seedlings.

关键词： 24-epibrassinolide 28-Homobrassinolide Antioxidative System Malondialdehyde Polyethylene Glycol-6000 Radish Water Stress

Interaction of Brassinolide with Auxin and Ethylene in Brassinolide-Enhanced Curvature/coiling of Primary Roots of Rice(Oryza sativa L.) Seedlings

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Interaction of Brassinolide with Auxin and Ethylene in Brass...