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20-hydroxyecdysone Improves Neuronal Differentiation of Adult Hippocampal Neural Stem Cells in High Power Microwave Radiation-Exposed Rats

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Biomedical and Environmental Sciences 2022年第6期35卷 504-517页

作者： LIU Jing Jing ZHANG Hong Yan CHEN Xin ZHANG Guang Bin LIN Jiang Kai FENG Hua CHU Wei Hua Department of Neurosurgery and Key Laboratory of Neurotrauma Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Department of Occupational Health Faculty of Preventive MedicineThird Military Medical University(Army Medical University)Chongqing 400038China

Objective The hippocampus is thought to be a vulnerable target of microwave *** aim of the present study was to investigate whether 20-hydroxyecdysone(20E)acted as a fate regulator of adult rat hippocampal neural stem cells(NSCs).Furthermore,we investigated if 20E attenuated high power microwave(HMP)radiation-induced learning and memory *** Sixty male Sprague-Dawley rats were randomly divided into three groups:normal controls,radiation treated,and radiation+20E *** in the radiation and radiation+20E treatment groups were exposed to HPM radiation from a microwave emission *** learning and memory abilities of the rats were assessed using the Morris water maze *** adult rat hippocampal NSCs were isolated in vitro and cultured to evaluate their proliferation and *** addition,hematoxylin&eosin staining,western blotting,and immunofluorescence were used to detect changes in the rat brain and the proliferation and differentiation of the adult rat hippocampal NSCs after HPM radiation *** The results showed that 20E induced neuronal differentiation of adult hippocampal NSCs from HPM radiation-exposed rats via the Wnt3a/β-catenin signaling pathway in ***,20E facilitated neurogenesis in the subgranular zone of the rat brain following HPM radiation *** of 20E attenuated learning and memory deficits in HPM radiation-exposed rats and frizzled-related protein(FRZB)reduced the 20E-induced nuclear translocation ofβ-catenin,while FRZB treatment also reversed 20E-induced neuronal differentiation of NSCs in *** These results suggested that 20E was a fate regulator of adult rat hippocampal NSCs,where it played a role in attenuating HPM radiation-induced learning and memory deficits.

关键词： High power microwave Hippocampus 20-hydroxyecdysone Learning and memory

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20-hydroxyecdysone from Dacrycarpus imbricatus bark inhibits the proliferation of acute myeloid leukemia cells

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Asian Pacific Journal of Tropical Medicine 2017年第2期10卷 151-153页

作者： Trinh Thi Thuy Nguyen Thanh Tam Nguyen Thi Hoang Anh Dang Viet Hau Dinh Thi Phong Le Quoc Thang Sabrina Adorisio Tran Van Sung Domenico V Delfino Institute of Chemistry Vietnam Academy of Science and Technology Vietnam National Museum of Nature Vietnam Academy of Science and Technology Hue University’s College of Educations Section of Pharmacology Department of MedicineUniversity of Perugia

Objective:To investigate the anti-proliferative effects of 20-hydroxyecdysone isolated from the bark of Dacrycarpus imbricatus(Blume) de ***:Column chromatography was used for isolation of compounds from plant *** structure of the isolated compound was identified by mass spectrometry and nuclear magnetic resonance techniques,including HSQC,HMBC,NOE-difference *** isolated compound was tested for its anti-proliferative activity in acute myeloid leukemia(AML) and OCI-AML ***:Compound 1 was isolated from the ethyl acetate fraction of Dacrycarpus imbricatus barks by column *** chemical structure was identified as 20-hydroxyecdysone(20HE),a cholestane-type ecdysteroid,by a combination of mass spectrometry and nuclear magnetic resonance spectrometric *** goal was to test the anti-proliferative activity of 20 HE using the OCI-AML cell line.20 HE significantly decreased OCI cell number at a concentration of 1 mg/mL,whereas lower concentrations were ***,this decrease was due to partial blockage of the G_1/S phase of the cell cycle,with a reduction of cells in the G_2M phase,not due to increased ***:This indicates that 20 HE significantly decreases the number of cells in the G_1/S phase of the cell cycle in human AML *** is the first time that the anti-proliferative activity of 20 HE against a human tumor cell line has been reported.

关键词： Dacrycarpus imbricatus Ecdysteroid 20-hydroxyecdysone Proliferation Acute myeloid leukemia

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Steroid hormone 20-hydroxyecdysone promotes CTL1-mediated cellular immunity in Helicoverpa armigera

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Insect Science 2021年第5期28卷 1399-1413页

作者： Gui-Jie Wang Wen-Wen Wang Yu Liu Lian-Qin Chai Guo-Xiu Wang Xu-Sheng Liu Yu-Feng Wang Jia-Lin Wang Hubei Key Laboratory of Genetic Regulation and Integrative Biology School of Life SciencesCentral China Normal UniversityWuhan 430079China School of Life Sciences Henan UniversityKaifengHenan475004China

Mermithid nematodes,such as Ovomermis sinensis,are used as biological control agents against many insect pests,including cotton bollworm(Helicoverpa armigera).However,given the host's robust immune system,the infection rate of *** is low,thus restricting its widespread *** understand the host defense mechanisms against mermithid nematodes,we identified and characterized a protein involved in the recognition of ***,the potential ***-binding protein C-type lectin 1(HaCTLla and/or HaCTLIb),which was eluted from the surface of *** after incubation with *** *** is homologous to the previously reported HaCTLla *** was predominantly expressed in hemocytes and was induced by the steroid hormone 20-hydroxyecdysone through ecdysone receptor(HaEcR)or ultraspiracle(HaUSP),or *** assays confirmed the interactions of the HaCTLI proteins with *** but not with Romanomermis wuchangensis,a parasitic nematode of ***,the HaCTL1 proteins were secreted into the hemocoel and promoted hemocyte-mediated encapsulation and phagocytosis.A knockdown of HaEcR and/or HaUSP resulted in compromised encapsulation and ***,HaCTLI appears to modulate cellular immunity in the defense against parasitic nematodes,and the 20-hydroxyecdysone-HaEcR-HaUSP complex is involved in regulating the process.

关键词： C-type lectin encapsulation phagocytosis 20-hydroxyecdysone

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Identification of LmUAP1 as a 20-hydroxyecdysone response gene in the chitin biosynthesis pathway from the migratory locust, Locusta migratoria

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Insect Science 2018年第2期25卷 211-221页

作者： Xiao-Jian Liu Ya-Wen Sun Da-Qi Li Sheng Li En-Bo Ma[] Jian-Zhen Zhang Research Institute of Applied Biology College of Life Science Shanxi University Taiyuan China Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

In Locusta migratoria, we found that two chitin biosynthesis genes, UDP N-acetylglucosamine pyrophosphorylase gene LmUAP1 and chitin synthase gene Lm- CHS1, are expressed mainly in the integument and are responsible for cuticle formation. However, whether these genes are regulated by 20-hydroxyecdysone （20E） is still largely unclear. Here, we showed the developmental expression pattern ofLmUAP1, LmCHS1 and the corresponding 20E titer during the last instar nymph stage of locust. RNA interference （RNAi） directed toward a common region of the two isoforms of LmEcR （LmEcRcom） reduced the expression level of LmUAP1, while there was no difference in the expression of LmCHS1. Meantime, injection of 20E in vivo induced the expression of LmUAP1 but not LmCHS1. Further, we found injection-based RNAi of LmEcRcom resulted in 100% mortality. The locusts failed to molt with no apolysis, and maintained in the nymph stage until death. In conclusion, our preliminary results indicated that LmUAP1 in the chitin biosynthesis pathway is a 20E late-response gene and LmEcR plays an essential role in locust growth and development, which could be a good potential target for RNAi-based pest control.

关键词： chitin synthesis Locusta migratoria regulation RNA interference 20-hydroxyecdysone

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Steroid hormone 20-hydroxyecdysone disturbs fat body lipid metabolism and negatively regulates gluconeogenesis in Hyphantria cunea larvae

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Insect Science 2023年第3期30卷 771-788页

作者： Sheng-Yu Zhang Han Gao Ankarjan Askar Xing-Peng Li Guo-Cai Zhang Tian-Zhong Jing Hang Zou Hao Guan Yun-He Zhao Chuan-Shan Zou School of Forestry Northeast Forestry UniversityHarbinChina Beiua University JilinChia

The steroid hormone 20-hydroxyecdysone(20E)has been described to regulate fat body lipid metabolism in insects,but its accurate regulatory mechanism,especially the crosstalk between 20E-induced lipid metabolism and gluconeogenesis remains largely ***,we specially investigated the effect of 20E on lipid metabolism and gluconeogenesis in the fat body of Hyphantria cunea larvae,a notorious pest in *** analysis showed that a total of 1907 lipid species were identified in the fat body of *** larvae assigned to 6 groups and 48 lipid *** differentially abundant lipids analysis showed a significant difference between 20E-treated and control samples,indicating that 20E caused a remarkable alteration of lipidomics profiles in the fat body of *** *** studies demonstrated that 20E accelerated fatty acidβ-oxidation,inhibited lipid synthesis,and promoted ***,the activities of pyruvate carboxylase,phosphoenolpyruvate carboxykinase,fructose-1,6-bisphosphatase,and glucose-6-phosphatase were dramatically suppressed by 20E in the fat body of *** *** well,the transcriptions of genes encoding these 4 rate-limiting gluconeogenic enzymes were significantly downregulated in the fat body of *** larvae after treatment with *** together,our results revealed that 20E disturbed fat body lipid homeostasis,accelerated fatty acidβ-oxidation and promoted lipolysis,but negatively regulated gluconeogenesis in *** *** findings might provide a new insight into hormonal regulation of glucose and lipid metabolism in insect fat body.

关键词： 20-hydroxyecdysone Hyphantria cunea fat body lipidomics analysis lipid metabolism gluconeogenesis

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Rapid Determination of Endogenous 20-hydroxyecdysone in Plants on MALDI-TOF/TOF Mass Spectrometry via Chemical Labeling Based on Boronate Affinity

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Journal of Analysis and Testing 2022年第3期6卷 318-326页

作者： Ya-Shun Chen Yu-Qi Feng College of Science Honghe UniversityMengzi 661100China Department of Chemistry Wuhan UniversityWuhan 430072China

20-hydroxyecdysone(20E)derived from plants has a wide range of physiological and pharmacological ef ects on animals and humans,and rapid and sensitive methods for screening of the endogenous 20E in plants are thus ***,a matrixassisted laser desorption/ionization time-of-f ight tandem mass spectrometry(MALDI-TOF/TOF MS)method is described for rapid and sensitive determination of endogenous 20E in *** is based on the use of the(3-(acridin-9-ylamino)phenyl)boronic acid(AYPBA)as the mass tag to assist the MS and tandem MS(MS^(n))analysis of 20E on MALDI-TOF/TOF *** linearity was obtained with a determination coef cient(R^(2))larger than 0.99 in the range of 0.025–2.5μΜ.The limit of detection(LOD)was 2.4 *** precision and accuracy were gained by intra-and inter-day analysis with relative standard deviations less than 19.5%and relative recoveries ranging from 85.7 to 105.2%.In addition,the AYPBA labeled 20E produced abundant characteristic fragment ions under the high energy collision-induced dissociation,which facilitated the identif cation of 20E by MS^(2)analysis on MALDI-TOF/TOF *** the method,we enabled the identif cation and quantif cation of endogenous 20E in four herbs including Cyanotis arachoidea,Achyranthes bidentata,Spinacia oleracea and Chenopodium quinoa willd.,demonstrating the feasibility of the proposed method for screening of the endogenous 20E in plants.

关键词： Matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry 20-hydroxyecdysone Chemical labeling Boronate affinity

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Epidermal collagenase activity and its induction by 20-hydroxyecdysone in the fiddler crab Uca pugilator

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Current Zoology 2009年第1期55卷 75-80页

作者： Erin A. TOUPS Enmin ZOU Department of Biological Sciences Nicholls State University

The epidermal collagenase activity and its induction by 20-hydroxyecdysone in Uca pugilator were investigated. Zymographic electrophoresis showed four bands of collagenase activity, 16, 19, 22 and 29 kDa in molecular weight, with the former two accounting for 60% and 36% , respectively, of the total collagenase activity. The collagenase activity varies during the molting cycle. Among the molt stages tested, Premolt Stage Do exhibited the highest epidermal collagenase activity for both the 16 and 19 kDa isoenzymes and, as the molt stage proceeded, the enzymatic activity of these two isoenzymes decreased, with the lowest activity for both found in Premolt Stage D3 -4 - Injection of 20-hydroxyecdysone significantly induced the activity of the 16 kDa eollagenase in the epidermis of Uca pugilator, suggesting that the activity of this isoenzyme is under molting hormone control. Although 20-hydroxyeedysone injection did not result in a statistically significant increase in the activity of the 19 kDa isoenzyme, a tendency of the induction was nonetheless demonstrated. This is the first report on epidermal collagenase activity and its induction by the molting hormone in a crustacean .

关键词： Collagenase Crustacean Epidermis Exoskeleton 20-hydroxyecdysone Uca pugilator

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Effects of Salinity Fluctuation Frequency on the Growth, Molting Rate and Hemolymph 20-hydroxyecdysone Concentration in Juvenile Chinese shrimp, Fenneropenaeus chinensis

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Journal of Ocean University of China 2009年第3期8卷 259-264页

作者： DING Sen WANG Fang SUN Hao DONG Shuanglin The Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao 266003 P. R. China China National Marine Data Information Service the State Oceanic Administration Tianjin 300171 P. R. China

This experiment was conducted to examine the effects of salinity fluctuation frequency on the growth, molting rate and hemolymph 20-hydroxyecdysone （20-HE） concentration for juvenile Fenneropenaeus chinensis with an initial wet body weight of 1.115 g±0.012 g. The salinity of the control treatment （represented by DO） was 28 throughout the experiment, whereas treatments D2, D4, D6 and D8 were subjected to different salinity fluctuation frequencies of 2, 4, 6 and 8 d, respectively. The range of salinity fluctuation was 4. The salinity in treatments D2, D4, D6 and D8 was kept at 28 for 2, 4, 6 and 8d, respectively; each original salinity was decreased abruptly to salinity 24, which first lasted for another 2 d and was then raised to its initial value 28. This constituted a salinity fluctuation cycle and afterwards the cycle repeated. It was found that： 1） The weight gain of shrimp in treatment D4 was 202.9% and significantly higher than that in treatment DO （P〈0.05）. The molting rate in treatment D2 was the lowest, while that in treatment D6 was the highest and significantly higher than those in treatments DO and D2 （P〈0.05）. 2） The hemolymph 20-HE concentration of shrimps in all treatments was at low levels and increased first gradually during the inter-molt period and then increased sharply. It reached a peak value during the pre-molt stage, and then decreased abruptly during the post-molt stage when the lowest level occurred. 3） During the post-molt stage, the hemolymph 20-HE concentration in treatment D2 was 7.47pg μL^-1 and significantly higher than that in treatment DO （P〈0.05）. There were, however, no significant differences in hemolymph 20-HE concentration among all five treatments during the inter-molt stage （P〉0.05）. During the pre-molt stage, the hemolymph 20-HE concentration in treatment D6 was significantly higher than that in treatment DO （P〈0.05）, whereas the hemolymph 20-HE concentration in treatment D2 was significantly lower than that in other treatmen

关键词： Fenneropenaeus chinensis Salinity fluctuation frequency Growth Molting rate 20-hydroxyecdysone

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Protective effects of 20-hydroxyecdysone on H_2 O_2-induced cytotoxicity in human neuroblastoma SH-SY5Y cells

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Journal of Chinese Pharmaceutical Sciences 2014年第1期23卷 33-38页

作者：司霞马卓陈月黄琳冯婉玉北京大学人民医院药剂科北京100044 北京大学医学部药学院药事管理与临床药学系北京100191

The aim of this study was to investigate the possible protective effects and mechanisms of 20-hydroxyecdysone, an insect steroid hormone, on HEO2-induced cytotoxicity in human neuroblastoma SH-SYSY cells. Pretreatment with 20-hydroxyecdysone significantly elevated the cell viability and decreased LDH leakage in H2O2-treated SH-SY5Y cells. 20-hydroxyecdysone also dramatically reduced malondialdehyde （MDA） contents and enhanced the superoxide dismutase （SOD） activities under oxidative stress conditions. Furthermore, 20-hydroxyecdysone pretreatment inhibited apoptosis by decreasing the Bax/Bcl-2 ratio and attenuating the activation of caspase-3. These results suggest that 20-hydroxyecdysone can protect SH-SYSY cells against H2O2-induced cytotoxicity and might potentially be used to treat neurodegenerative diseases induced by oxidative stress and anontosis.

关键词： 20-hydroxyecdysone H2O2 SH-SY5Y cells Oxidative stress Apoptosis Neurodegenerative disease

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The Modulation of Trehalose Metabolism by 20-hydroxyecdysone in Antheraea pernyi(Lepidoptera:Saturniidae) During Its Diapause Termination and Post-Termination Period

The Modulation of Trehalose Metabolism by 20-Hydroxyecdysone...

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中国生物化学与分子生物学会2020全国学术在线会议

作者： Ya-Na Li Yu-Bo Liu Xue-Qin Xie Jia-Ning Zhang Wen-Li Li School of Bioengineering Dalian University of Technology School of Life Science and Medicine Dalian University of Technology

Trehalose plays a crucial role in the diapause process of many insects,serving as an energy source and a stress *** accumulation has been reported in diapause pupae of Antheraea pernyi;however,trehalose metabolic regulatory mechanisms associated with diapause termination remain ***,we showed that the enhanced trehalose catabolism was associated with an increase in endogenous 20-hydroxyecdysone(20 E) in hemolymph of *** pupae during their diapause termination and post-termination *** of 20 E increased the mRNA level of trehalase 1 A(ApTre-1 A) and trehalase 2(ApTre-2) of *** diapause pupae in a dose-dependent manner but did not affect the mRNA level of trehalase1 B(ApTre-1 B).Meanwhile,exogenous 20 E increased the enzyme activities of soluble and membrane-bound trehalase,leading to a decline in hemolymph ***,the expression of ApTre-1 A and ApTre-2 were down-regulated after the ecdysone receptor gene(ApEcRB1) was silenced by RNA interference or by injection of an ecdysone receptor antagonist cucurbitacin B(CucB),which inhibits the 20 E ***,CucB treatment delayed adult emergence,which suggests that ApEcRB1 might be involved in regulating pupal-adult development of *** by mediating ApTre-1 A and ApTre-2 *** study provides an overview of the changes in the expression and activity of different trehalase enzymes in *** in response to 20 E,confirming the important role of 20 E in controlling trehalose catabolism during *** diapause termination and post-termination period.

关键词： 20-hydroxyecdysone ecdysone receptor trehalase diapause termination post-termtation

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