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Effects of ELF magnetic fields on protein expression profile of human breast cancer cell MCF7

Science China(Life Sciences) 2005年第5期48卷 506-514页

作者： LI Han1, ZENG Qunli1, 2, WENG Yu1, LU deqiang1, JIANG Huai1 & XU Zhengping1, 2 1. Bioelectromagnetics Laboratory, Zhejiang University , Hangzhou 310031, China 2. Research Center for Environmental Genomics, Zhejiang University School of Medicine, Hangzhou 310031, China Bioelectromagnetics Laboratory Zhejiang University Hangzhou 310031 China Bioelectromagnetics Laboratory Zhejiang University Hangzhou 310031 China Research Center for Environmental Genomics Zhejiang University School of Medicine Hangzhou 310031 China

Extremely Low Frequency Magnetic Fields (ELF MF) has been considered as a “possible human carcinogen” by International Agency for Research on Cancer (IARC) while credible mechanisms of its carcinogenicity remain unknown. In this study, a proteomics approach was employed to investigate the changes of protein expression profile induced by ELF MF in human breast cancer cell line MCF7, in order to determine ELF MF-responsive proteins. MCF7 cells were exposed to 50 Hz, 0.4 mT ELF MF for 24 h and the changes of protein profile were examined using two dimensional electrophoresis. Up to 6 spots have been statistically signifi-cantly altered (their expression levels were changed at least 5 fold up or down) compared with sham-exposed group. 19 ones were only detected in exposure group while 19 ones were missing. Three proteins were identified by LC-IT Tandem MS as RNA binding protein regulatory subunit、Proteasome subunit beta type 7 precursor and Translationally Controlled Tumor Protein. Our finding showed that 50 Hz, 0.4 mT ELF MF alternates the protein profile of MCF7 cell and may affect many physiological functions of normal cell and 2-de coupled with MS is a promising ap-proach to elucidating cellular effects of electromagnetic fields.

关键词： ELF MF, MCF7, 2-de, MS, protein expression profile.

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