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A Novel 2-dimensional multiplex qpcr Assay for Single-Tube detection of Nine Human Herpesviruses

Virologica Sinica 2021年第4期36卷 746-754页

作者： Yingxue Li Zhenzhou Wan Lulu Zuo Shenwei Li Honglian Liu Yingying Ma Lianqun Zhou Xia Jin Yuye Li Chiyu Zhang Shanghai Public Health Clinical Center Fudan UniversityShanghai201508China CAS Key Laboratory of Bio-Medical diagnostics Suzhou Institute of Biomedical Engineering and TechnologyChinese Academy of SciencesSuzhou215163China School of Biomedical Engineering University of Science and Technology of ChinaHefei260026China Medical Laboratory of Taizhou Fourth People's Hospital Taizhou225300China Viral Hemorrhagic Fevers Research Unit Institut Pasteur of ShanghaiChinese Academy of SciencesShanghai200031China Shanghai International Travel Healthcare Center Shanghai200335China department of dermatology and Venereology First Affiliated Hospital of Kunming Medical UniversityKunming650032China

Human herpesviruses are double-stranded dNA viruses that are classified into nine *** than 90%of adults are ever infected with one or more *** symptoms of infection with different herpesviruses are diverse ranging from mild or asymptomatic infections to deadly diseases such as aggressive lymphomas and *** and accurate detection of herpesvirus infection is critical for clinical management and *** this study,we established a single-tube nonuple qpcr assay for detection of all nine herpesviruses using a 2-d multiplex qpcr method with a house-keeping gene as the internal *** novel assay can detect and distinguish different herpesviruses with 30 to 300 copies per 25µL single-tube reaction,and does not cross-react with 20 other human viruses,including dNA and RNA *** robustness of the novel assay was evaluated using 170 clinical *** novel assay showed a high consistency(100%)with the single qpcr assay for HHVs *** features of simple,rapid,high sensitivity,specificity,and low cost make this assay a high potential to be widely used in clinical diagnosis and patient treatment.

关键词： Human herpesviruses(HHVs) 2-d multiplex qpcr Melting temperature(Tm) Fluorescence probe Melting curve

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