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Monitoring and identification of spoilage-related microorganisms in braised chicken with modified atmosphere packaging during refrigerated storage

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Food science and Human Wellness 2023年第1期12卷 28-34页

作者： Yang Lei Yali Zhang Yiqun Cheng Jichao Huang Ming Huang Key Laboratory of Meat Processing and Quality Control Ministry of EducationCollege of Food Science and TechnologyNanjing Agricultural UniversityNanjing 210095China National R&D Center for Poultry Processing Jiangsu Research Center for Livestock and Poultry Products Processing Engineering TechnologyNanjing Huangjiaoshou Food Science and Technology Co.Ltd.Nanjing 211225China College of Environmental science&Engineering Institute of Functional FoodAnhui Normal UniversityWuhu 241000China College of Engineering Nanjing Agricultural UniversityNanjing 210031China

This study mainly monitored the dominant bacterial populations and identified the spoilage-related microorganisms of braised chicken meat stored under different CO_(2)-modified atmosphere packaging(MAP)during refrigerated storage using a culture-dependent method and 16s rdna *** quality changes and shelf life of the meat were also *** growth rate of total viable count(TVC)in braised chicken was slower with an increase of CO_(2) content in MAP,which also occurred in the remaining bacterial species monitored(lactic acid bacteria,Pseudomonas spp.,Brochothrix thermosphacta).The MAP exerted beneficial effects on the quality of braised chicken,as demonstrated by retarding the production of total volatile basic nitrogen(TVB-N)and delaying lipid oxidation(TBARs test).A total of 14 isolates were identified from braised chickens with different packaging at the end of storage,these included ***(6 isolates),***(2 isolates),Enterococcus faecalis(3 isolates),***(2 isolates),staphylococcus equorum(1 isolate).

关键词： Braised chicken Modified atmosphere packaging(MAP) 16s rdna identification shelf-life

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Isolation,screening and identification of Antagonistic Actinomycetes against Ustilago scitaminea syd

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Agricultural Biotechnology 2018年第4期7卷 225-228页

作者： Gengfeng ZHAO Guoru XIONG Wenzhi WANG Linbo sHEN shuzhen ZHANG Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences College of Basic Medicine and Life science Hainan Medical University

[Objectives] This study was conducted to obtain actinomycetes strains having antagonistic effect on Ustilago scitaminea syd.[Methods] At first, actinomycetes strains were isolated from 22 soil samples in Hainan sugarcane regions. Then, antagonistic actinomycetes against U. scitaminea were screened by confrontation culture. Finally, the taxonomic status of antagonistic actinomycetes was determined using 16s rdna.[Results] From the 22 samples, 984 actinomycetes strains were isolated. From all the isolated strains, 23 antagonistic actinomycetes strains were obtained through primary screening, and one strains with better antagonistic effect was then obtained through secondary screening, and designated FAs. 16s rdna identification showed that strain FAs shared 99% sequence similarity with streptomyces cealestis Us24. A phylogenetic tree was built with MAGE 7.0 software, and the results showed that strain FAs had the shortest genetic distance with s. caelestis Us24. Therefore, the actinomycetes FAs was determined as s. caelestis .[Conclusions] This study provides a new biocontrol method for the biological control of sugarcane smut, thereby ensuring sustainable development of sugarcane industry and sugar industry.

关键词： sugarcane smut 16s rdna identification streptomyces caelestis Antagonistic effect

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