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*** stem cells repair retinal vascular damage in retinopathy of prematurity mouse model

Annals of Eye Science 2018年第1期 434-434页

作者： Baraa Noueihed Jose carlos Rivera Sylvain chemtob Department of Pharmacology McGill UniversityMontréalQCCanada Research center Hôpital Maisonneuve-Rosemont Universitéde MontréalMontréalQCCanada Research center cHU Sainte-Justine Universitéde MontréalMontréalQCCanada Department of Pharmacology Universitéde MontréalMontréalQCCanada Department of Ophthalmology Universitéde MontréalMontréalQCCanada Department of Pediatrics Universitéde MontréalMontréalQCCanada

Background:Retinopathy of prematurity is a leading cause of visual impairment and blindness in *** of premature babies to the hyperoxic extrauterine environment leads to vaso-obliteration(VO),followed by ischemia,and subsequently pathological intravitreal neovascularization(NV)in the immature *** treatments target only aberrant intravitreal vessel growth without repopulating the avascular regions of the ***,there is a dire need of new therapies that arrest pathological NV and promote normal retinal *** stem cells(MScs)have shown the ability to migrate to the damaged tissue in different animal models and enhance ***,therefore,investigated whether MScs can promote vascular repair in a mouse model of ***:Oxygen-induced retinopathy(OIR)model was used *** day 7(P7)mice were subjected to 75%O2 until P12 to induce VO followed by 5 days of room air leading to *** bone-derived MScs were isolated from adult c57BL/6 mice and cultured either in hypoxia(5%O2)or normoxia(21%O2).conditioned media(cM)was collected 24 hours later and injected intravitreally in P12 OIR retinas to assess vascular *** determine possible factors involved in MSc-induced revascularization,gene expression analysis was performed on P17 OIR *** vitro,we investigated the effect of MSc-cM on microglial polarization using quantitative PcR and flow ***:Hypoxic MSc-cM significantly(Pcreased both VO and NV areas in comparison to the normoxic *** of IGF-1 and VEGF were significantly high in MSc-injected OIR ***,gene expression levels of pro-inflammatory cytokines(IL-1β,TNFα)dropped whereas levels of anti-inflammatory cytokines(IL-10,IL-4)*** of pro-inflammatory M1 microglia with MSc-cM decreased the gene expression of IL-1βand TNFα,and iNOS(M1 marker)at the transcript and protein ***:In this study,we demonstrated that MScs

关键词： Optokinetics visual evoked potentials Nogo-A 11c7

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***-A neutralisation improves vision recovery after retinal injury

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Annals of Eye Science 2018年第1期 433-433页

作者： Julius Baya Mdzomba Sandrine Joly Léa Rodriguez Frédéric Bretzner Vincent Pernet cUO-Recherche Centre de recherche du CHU de Québec et Département d'ophtalmologieFacultéde médecineUniversitéLavalQuébecQuébecCanada cUO-Recherche Centre de Recherche du CHU de QuébecDépartement de psychiatrie et neurosciencesFacultéde MédecineAxe Neurosciences et UniversitéLavalQuébecQuébecCanada

Background:In glaucoma and after an ischemic injury of the retina,excessive activation of N-Methyl-D-Aspartate receptors,a type of glutamatergic receptors,induces the death of retinal ganglion cells and an irreversible vision *** painless loss of retinal cells does not allow for a swift diagnostic and treatment of retinal *** is no efficient therapy to improve retinal functions in this *** order to develop new therapeutic approaches for retinal injury,we propose,in this study,to stimulate neuronal plasticity of the visual system by neutralising the glial protein,*** inhibitory action of this protein on axonal regeneration is well known in spinal cord injuries but not in the visual *** thus studied the function of Nogo-A in vision recovery in ***:Nogo-A activities were chronically blocked by deleting its gene in KO mice and acutely,by intravitreal injections of an antibody known as *** retina lesions were done by injection of 0.5 or 5 nmol of NMDA in the vitreous humor.A PBS buffer was administered in control *** visual system functions were accessed with an optokinetic test in awake mice,by electroretinography(ERG)in the eye and visual evoked potentials in the visual *** survival of retinal ganglion cells,amacrine and bipolar cells was evaluated on histological sections by *** in expression of Nogo-A,its receptors and neuronal plasticity associated molecules were observed by Western blot and ***:At NMDA doses of≤0.5 nmol,Nogo-A KO mice show a recovery of optokinetic responses much faster than in WT ***,a single injection of the antibody,11c7 was sufficient to improve VEPs of NMDA injured animals as compared to control ***,ERGs showed that a dose of 0.5 nmol induced retinal lesions limited to the ganglion cell layer,with significant changes to the VEPs but without influencing photoreceptors and inner nuclear layer cells ***,5 n

关键词： Optokinetics visual evoked potentials Nogo-A 11c7

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