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Diagnosis of Helicobacterpyloriinfection and diseases associated with Helicobacter pylori by Helicobacter pylori outer membrane proteins

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World Journal of Gastroenterology 2004年第23期10卷 3464-3469页

作者： ZhengJiang Ai-LongHuang Xiao-HongTao Pi-LongWang DepartmentofGastroenterology theFirstAffiliatedHospitalChongqingUniversityofMedicalSciencesChongqing400016China InstituteofViralHepatitis ChongqingUniversityofMedicalSciencesChongqing400010China

AIM:TO examine the serological response of patients with upper gastrointestinal diseases and Helicobocter pylori(H pylon) infection to two Hpyloriouter membrane proteins(OMPs) (M,18 000 and M,26 000)acquired by gene recombinant technique,and to determine the diagnostic significance of serological tests derived from these OMPs. METHODS:Recombinant vectors encoding the two Hpylori OMPs were used to transform and express in BL21(DE3) *** purification with Ni^(2+)-NTA agarose resin,colloid gold kits were prepared with purified recombinant proteins to detect H pylori infectJon and H pylori-associated diseases by the immunity-marker *** selected 150 patients with Hpyloriinfection and digestive symptoms without previous treatment,induding chronic gastritis(n=60),duodenal ulcer (n=30),gastric ulcer(n=30),and gastric cancer(n=30). As controls,33 Hpylori-negative healthy volunteers were also *** samples were collected from all subjects,and the antibodies to specific proteins of H pylori were tested with the colloid gold test *** sensitivity, specificity and accuracy of the colloid gold tests were evaluated,by using the combination of standard diagnostic methods(^(13)C urea breath test and bacteria culture)and classic enzyme-linked immunosorbent assay(ELISA)as reference. RESULTS:After purification with Ni^(2+)-NTA agarose resin, the purity of recombinant fusion proteins was about 95%. The recombinant fusion proteins were recognized by the specific monodonal antibodies against the two Hpylori OMPs, as demonstrated by the *** the 150 serum samples from patients infected with Hpylori 141(94.0%)responded positively to the recombinant protein with M_126 000,while the seropositive rates were 95.0%,96.7%,96.7% and 90.0% for patients with H pylori-associated chronic gastritis, duodenal ulcer,gastric ulcer,and gastric cancer respectively. The sensitivity,specificity,and accuracy of the colloid gold kit with M_1 26 000 protein were 94.0%,97.0%,an

关键词：诊断哈比特属幽门菌伴随物膜蛋白重组器官

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Primary targeting of recombinant Fv-immunotoxin hscFv_(25)-mTNFα against hepatocellular carcinoma

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World Journal of Gastroenterology 2004年第13期10卷 1872-1875页

作者： JingZhang Yan-FangLiu Shou-JingYang QingQiao HongCheng Chuan-ShanZhang Fu-ChengMa Hua-ZhangGuo DepartmentofPathology XijingHospitalFourthMilitaryMedicalUniversityXi'an710033ShaanxiProvinceChina DepartmentofGeneralSurgery TangduHospitalFourthMilitaryMedicalUniversityXi'an710038ShaanxiProvinceChina

AIM: To obtain human recombinant Fv-immunotoxin hscFv25-mTNFα (mutant human TNFαfused to human scFv25) against hepatocellular carcinoma (HCC).METHODS: Two relevant sites of enzymatic digestion were added to mTNFα by PCR. mTNFα was linked to the 3' end of hscFv25 in pGEX4T-1 vector. This anti-HCC recombinant Fv-immunotoxin hscFv25-mTNFα was expressed in Escherichia coliand purified from inclusions. After purified by glutathione-S-transferase affinity chromatography and thrombin digestion, it was identified by electrophoresis and Western blot. And then, the purified recombinant Fvimmunotoxin was injected into nude mice with HCC xenografts through their tail veins, mTNFα protein and PBS were used as control at the same time. After treated for two weeks, nude mice were executed. The bulk and weight of tumors were observed. The tumor tissues were stained by immunohistochemical method with TNFα ***: The expression ratio of recombinant Fv-immunotoxin hscFv25-mTNFα was 12% of bacterial protein. The result of tumor restraining trials of hscFv25-mTNFα showed 2/5 complete remission and 3/5 partial remission, mTNFα restraining trials showed 5/5 partial remission. The therapeutic result of hscFv25-mTNFα was better than that of mTNFα (F=8.70, P

关键词：重组器官 Fv-抗毒素 hscFv25-mTNFα 肝细胞癌肿瘤 HCC

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Effects and Safety of High-efficiency Gene and Highlevel Expression of RA538 in Human Gastric Carcinoma Cells by Recombinant

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胃肠病学 2000年第B8期5卷 191-192页

作者： ChenJie-ping LinChen XuCai-pu WuMing Dept.ofGastroenterology SouthwestHospitalTheThirdMilitaryMedicalUniversityChongqing400038China NationalLaboratoryofMolecularOncology Dept.ofCellBiologyCancerInstitutePekingUnionMedicalCollegeBeijing100021China

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Oncosuppressive Effects of Natural and Recombinant Parvoviruses

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胃肠病学 2000年第B8期5卷 42-43页

作者： JeanRommelaere AppliedTumorVirology Abt.Abt.F0100andINSERMU375DeutschesKrebsforschunszentrumGermany

The infection of animals with certain autonomous parvoviruses has been shown to have an onco-sup-pressive effect, whilst the viruses themselves have a preferred tropism for transformed cells (oncotropism). However, in many instances, the level of this response is not sufficient for complete suppression of tumorigenesis. Our goal is to enhance the antitumor potential by combining both the intrinsic oncosuppressive and oncotropic determinants of the virus with an additional therapeutic gene in a recombinant parvoviral vector.

关键词：重组细胞病毒抑制作用肿瘤重组器官

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Cloning and expression of mouse peroxiredoxin I in 1EC-6 Cells

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World Journal of Gastroenterology 2004年第14期10卷 2109-2112页

作者： BoZhang Yong-PingSu TaoWang Feng-ChaoWang Guo-PingAi HuiXu Jun-PingWang Yue-ShengHuang Jian-XinJiang InstituteofCombinedInjuryofPLA StateKeyLaboratoryofTraumaBurnsandCombinedInjuryThirdMilitaryMedicalUniversityChongqing400038China

AIM: To clone and express mouse peroxiredoxin Ⅰ in IEC-6 ***: Total RNAs were isolated from cultured IEC-6 cells, and the coding region of peroxiredoxin I was amplified by RT-PCR. After it was cloned into T-vector and sequenced,pSG5 was used to transiently express peroxiredoxin I in IEC-6 by liposome-mediated transfection, and the expression of peroxiredoxin I was evaluated by RT-PCRand Western blot. RESULTS: A DNA fragment about 750 bp was amplified from total RNAs of IEC-6 cells using specific primers of peroxiredoxin Ⅰ. The sequencing confirmed the coding region was successfully cloned into T-vector, which was completely coincident with the sequence in GeneBank. After the EcoRI-BamHI fragment of T-vector containing peroxiredoxin I was inserted into pSG5, the recombinant plasmid was transferred to IEC-6 cells. RT-PCR assay showed that a DNA fragment of 930 bp could be amplified,which indicated the transcription of pSG5-Prx. Western blot confirmed the expression of peroxiredoxin Ⅰ in IEC-6 ***: Mouse peroxiredoxin I can be successfully expressed in IEC-6 cells.

关键词：无性繁殖基因表达老鼠 peroxiredoxinⅠ IEC-6细胞重组器官

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Effects of the Recombinant Plasmid of Interstitial Collagenase on Experimental Rat Liver Fibrosis

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胃肠病学 2000年第B8期5卷 128-128页

作者： YangChang-qing WangJi-yao GuoJin-sheng LiuJian-jun ZhangChi DivisionofGastroenterology ZhongshanHospitalShanghaiMedicalUniversityShanghai200032China

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Immunization with Recombinant HpaA Generate Protection against Helicobacter pylori Infection

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胃肠病学 2000年第B8期5卷 111-111页

作者： ChenYe ZhouDian-yuan WangJi-de YeGin-an ZhangWan-dai PLAInstituteforDigestiveMedicine TheFirstMilitaryMedicalUniversityGuangzhou510515China

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