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旅行医学科学 2011年第2期17卷 40-40页

作者：李忠吉林省肿瘤医院放疗科

目的通过对细胞凋亡情况进行检测,对比探讨喉癌自发性及放射诱发凋亡情况,以及两者之间的关系。从而分析喉癌的最佳放射治疗剂量。通过研究喉癌自发性与放射诱发细胞凋亡关系及其临床应用。观察喉癌放疗前及放疗中细胞凋亡的变化及其意... 详细信息

目的通过对细胞凋亡情况进行检测,对比探讨喉癌自发性及放射诱发凋亡情况,以及两者之间的关系。从而分析喉癌的最佳放射治疗剂量。通过研究喉癌自发性与放射诱发细胞凋亡关系及其临床应用。观察喉癌放疗前及放疗中细胞凋亡的变化及其意义,从细胞凋亡的角度重新认识喉癌,从而指导临床喉癌放疗剂量与水准,对于早期喉癌患者在保存其各种生理功能的前提下治疗甚至治愈肿瘤。

关键词：喉癌患者细胞凋亡放射诱发自发性放射治疗剂量临床应用诱发凋亡放疗剂量

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Effect of apoptosis on gastric adenocarcinoma cell line SGC-7901 induced by cis-9,trans-11-conjugated linoleic acid

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World Journal of Gastroenterology 2002年第6期8卷 999-1004页

作者： Jia-RenLiu Bing-QingChen Yan-MeiYang Xuan-LingWang Ying-BenXue FoodScienceandToxicology DepartmentofFoodScienceComellUniversityIthacaNY14853-7201USA PublicHealthColleng HarbinMedicalUniversityHarbin150001HeilongjiangProvinceChina

AIM: To determine the effect of apoptosis on gastric cancer cells (SGC-7901) induced by cis-9, trans-11-conjugated linoleic acid (c9, t11-CLA) and its possible mechanism in the inhibition of cancer cells ***: Using cell culture, flow cytometery and immunocytochemical techniques, we examined the cell growth, frequency of apoptosis and distribution of cell cycle,expression of ki67, bcl-2, Fas, and c-myc of SGC-7901 cells which were treated with various c9, t11-CLA concentrations (25,50,100 and 200 μmol@L-1) of c9, t11-CLA for 24h and 48 h,with a negative control (0.1% ethanol).RESULTS: The growth of SGC-7901 cells was inhibited by c9,t11-CLA. Eight days after treatment with various concentrations of c9,t11-CLA, as mentioned above, the inhibition rates were 5.9 %, 20.2 %,75.6 % and 82.4 %, respectively. The frequency of apoptosis on SGC-7901 cells induced by different concentrations of c9, t11-CLA (except for 25 μmol@L-1, 24 h) was significantly greater than that in the negative control (P＜0.01). To further investigate the influence of the cell cycle progression, we found that apoptosis induced by c9, t11-CLA may be involved in blocking the cell cycle of SGC-7901 cells. Immunocytochemical staining demonstrated that SGC-7901 cells preincubated in media supplemented with different c9, t11-CLA concentrations for various time periods significantly decreased the expressions of ki67 (the expression rates were 18.70-3.20 %, at 24 h and 8.10-0.20 % at 48 h, respectively), bd-2 (4.30-0.15 % at 24 h and 8.05 %-0 at 48 h),and c-myc(4.85-2.20 % at 24 h and 4.75-0.30 % at 48 h) as compared with those in the controls (the expressions of ki67, bcl-2, and c-mycwere 15.1% at 24 h and 13.5 % at 48 h, 6.80 % at 24 h and 8.00 % at 48 h,5.50 % at 24 h and 5.30 % at 48 h, respectively) (P＜0.01),whereas the expressions of Fas were increased (0.60-2.75 %,24 h and 0.45-5.95 %, 48 h).CONCLUSION: The growth and proliferation of SGC-7901 cells are inhibited by cg, t11-CLA via blocking

关键词：胃腺癌细胞系 SGC-7901 亚麻酸衍生物 cis-9 trans-11 诱发凋亡

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Roles of Fas signaling pathway in vitamin E succinateinduced apoptosis in human gastric cancer SGC-7901 cells

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World Journal of Gastroenterology 2002年第6期8卷 982-986页

作者： Kun Wu Yao Li Yan Zhao Yu-Juan Shah Wei Xia Lan Zhao,Department of Nutrition and Food Hygiene,Public Health School,Harbin Medical University,Harbin 150001,Heilongjiang Province,China Wei-Ping Yu,Genetics Institute,Texas University of USA,Austin,USA Department of Nutrition and Food Hygiene Public Health School Harbin Medical University Heilongjiang Province China. wukun@***

AIM: To investigate the roles of Fas signaling pathway in vitamin E succinate-induced apoptosis in human gastric cancer SGC-7901 ***: Human gastric cancer SGC-7901 cells were treated with VES at 5, 10, 20 mg@L-1, succinic acid and vitamin E as vehicle control and condition media only as untreated (UT) control. Apoptotic morphology was observed by DAPI staining. Western blot analysis was applied to measure the expression of Fas, FADD and caspase-8 proteins. After the cells were transiently transfected with Fas and FADD antisense oligonucleotides, respectively, caspase-8 activity was determined by flurometric ***: The morphologically apoptotic changes were observed after VES treatment by DAPI staining. 23.7 % and 89.6 % apoptosis occurred after 24 h and 48 h of 20 mg@L-1 VES treatment, respectively. The protein levels of Fas, FADD and caspase-8 were evidently increased in a dose-dependent manner after 24 h of VES treatment. The blockage of Fas by transfection with Fas antisense oligonucleotides obviously inhibited the expression of FADD protein. After SGC-7901 cells were transfected with Fas and FADD antisense oligonucleotides, caspase-8 activity was obviously decreased (P＜0.01), whereas Fas blocked more than ***: VES-induced apoptosis in human gastric cancer SGC-7901 cells involves Fas signaling pathway including the interaction of Fas, FADD and caspase-8.

关键词：人胃癌细胞 SGC-7091细胞系 Ve琥珀酸酯维生素E 诱发凋亡 Fas信号通路作用机制

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