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Optimization of cultural conditions for thermostable β-1,3-1,4-glucanase production by Bacillus subtilis ZJF-IA5

Journal of Zhejiang University Science 2003年第6期4卷 719-726页

作者：何国庆汤兴俊 MUKHTARA.M.Ali 陈启和 CollegeofBiosystemEngineeringandFoodScience ZhejiangUniversityHangzhou310029China

The optimization of cultural conditions for β-glucanase production by Bacillus subtilis ZJF-1A5 was investigated in flask trials. Temperature had great effect on β-glucanasc production which maximized atoptimal temperature of 37℃ and decreased significantly when temperature was over 37℃ . Charge quantity affected β-glucanasc production significantly. Adding oxygen vector N-dodecanc or acetic ether benefited β-glucanasc production, but it depended on the concentration and charge quantity. The results of fractional factorialdesign showed that age and size of inoculum and shaking speed were the key factors affecting β-glueanase production and the cultivation time span to reach the highest β-glucanasc activity. The optimal cultural conditionsfor p-glucanase production obtained with CCD were as follows: inoculum age and size (16 h, 3.82% (v/v)),shaking speed 210 r/rain, charge quantity of 30 mL in 250 mL flask and initial pH 7.0, cultured at 37℃ for 50 h. Repeated experimental results accorded with those predicted by a second-order polynomial model. The amount of p-glucanase, a-amylase and neutral protease produced by B subtills ZJF-1A5 was associated partially with cell growth. Those three enzymes' activities increased following the cell growth and increased signifi-cantly when cells entered the stationary phase.

关键词： β-1,3-1,4-葡聚糖酶枯草杆菌表面响应方法热稳定性发酵生产最优条件

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Medium Optimization of Tetraselmis sp.-1 Using Response Surface Methodology

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High Technology Letters 2003年第2期9卷 27-30页

作者：沈继红 Yu Junhong Lin Xuezheng Li Guangyou Liu Fayi First Institute of Oceanography SOA Qingdao 266061 P.R.China

Response surface methodology (RSM) is used to optimize the medium of Tetraselmis sp.-1 which is cell fused microalgae capable of growing under mixotrophic condition. Empirical models are developed to describe the relationships between the operating variables (glucose, urea, sodium dehydrogenate phosphate, sodium chloride) and responses (cell density). Statistical analysis indicates that glucose and urea have significant effects on the microalgae cell density, but other two factors (sodium dehydrogenate phosphate, sodium chloride) have no obvious effect. The path of steepest ascent is used to approach the optimal region of medium composition. Optimal cell density (2.638 g dry weight/L) was reached when the operating conditions were glucose concentration (30.75 %), urea concentration (0.440 g/L), sodium dehydrogenate phosphate (15 mg/L) and sodium chloride (28 g/L).

关键词：微藻细胞融合表面响应方法媒质最优化氯化钠磷酸钠

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