Non-synchronous flowering and low natural pollination make breeding between clones of Heveabrasiliensis difficult. Pollen storage in liquid nitrogen (-196 oC) was an ideal strategy for long-term preservation for hybri...
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Non-synchronous flowering and low natural pollination make breeding between clones of Heveabrasiliensis difficult. Pollen storage in liquid nitrogen (-196 oC) was an ideal strategy for long-term preservation for hybridization. This study were investigated pollen viability of clone RRIM 600 and RRIT 251 after long storage in liquid nitrogen. Anther of both rubber clone were dried to 7% and 11% of moisture content levels before storage in liquid nitrogen. Pollen viability was testing by in-vitro germination in solution media and 2, 3, 5-triphenyl-tetrazolium-chloridestained. The results showed the moisture content at 11% storage 180 days were found pollen stained and pollen germination higher than at 7% of moisture content. This were the pollen stained 2.00% in clone RRIM 600 and 2.06 % in clone RRIT 251 while pollen germination were 1.31 % in clone RRIM 600 and 1.38% in clone RRIT. Besides, the pollen storage for 90 days in both moisture contents having correlated to the duration of Hevea flowering period were found pollen viability more than 10%. This finding will be the base information on the utilization of breeders for hybridization of Hevea varieties.
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