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Establishment of a pig model with enteric and portal venous drainage of pancreatoduodenal transplantation

World Journal of Gastroenterology 2005年第35期11卷 5475-5479页

作者： Zhao-Da Zhang Fang-Hai Han Ling-Xiang Meng Third GeneralDepartment West China Hospital of Sichuan University Chengdu610041 Sichuan Province China

AIM: To establish the pig model of pancreatoduodenal transplantation with enteric drainage (ED) and portal venous drainage (PVD).METHODS: Forty-six hybrid Landrace pigs were divided into two groups (donors and recipients) randomly, and pancreatoduodenal allotransplantation was performed.Donors were perfused via abdominal aorta without clamping the portal venous outflow with UW solution at 80-100 cm H2O after heparinization. Whole pancreatoduodenal grafts were harvested with segments of abdominal aorta and portal vein, and shaped under 4 ℃ UW solution. Then, end-to-end anastomosis was performed with the donor iliac artery bifurcation Y graft to the recipient superior mesenteric artery and celiac artery. Furthermore, type Ⅰ diabetes model was made by removal of the recipient pancreas. The venous anastomosis was reconstructed between the donor portal vein and the recipient superior mesentery vein. Meanwhile, end-toside anastomosis was performed with the donor common iliac artery bifurcation Y graft to the recipient abdominal aorta, and side-to-side intestinal anastomosis was performed between the donor duodenum and the recipient jejunum. External jugular vein was intubated for transfusion. Levels of plasma glucose, insulin and glucagon were measured during the operation and on the 1st, 3rd, 5th, and 7th d after operation.RESULTS: Pancreatoduodenal allotransplantation was performed on 23 pigs of which 1 died of complication of anesthesia. The success rate of operation was 95.6%.Complications of operation occurred in two cases in which one was phlebothrombosis with an incidence of 4.6%,and the other was duodenojejunal anastomotic leak with an incidence of 4.6%. The level of plasma glucose decreased within 30 min, after removal of pancreas and recovered on the 2nd d after operation. The level of plasma insulin and glucagon increased within 30 min after removal of pancreas and recovered on the 2nd d after operation.Rejection occurred on the 1st d and reached the worst level on the 7th d after transplantation, without ch

关键词：猪动物实验肠静脉肠移植胰腺损伤并发症

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Intestinal colonization with Candida albicans and mucosal immunity

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World Journal of Gastroenterology 2004年第14期10卷 2124-2126页

作者： Xiao-DongBai Xian-HuaLiu Qing-YingTong CentralLaboratory GeneralHospitalofChinesePeople'sArmedPoliceForceBeijing100039China MedicalAffairDepartment GeneralHospitalofChinesePeople'sArmedPoliceForceBeijing100039China DepartmentofBurnSurgery GeneralHospitalofChinesePeople'sArmedPoliceForceBeiiing100039China

AIM: To observe the relationship between intestinal lumen colonization with Candida albicans and mucosal secretory IgA (sIgA).METHODS: A total of 82 specific-pathogen-free mice were divided randomly into control and colonization groups. After Candida albicans were inoculated into specific-pathogen-free mice, the number of Candida albicans adhering to cecum and mucosal membrane was counted. The lymphocyte proliferation in Peyer's patch and in lamina propria was shown by BrdU incorporation, while mucosal sIgA (surface membrane) isotype switch in Peyer's patch was investigated. IgA plasma cells in lamina propria were observed by immunohistochemical staining. Specific IgA antibodies to Candida albicans were measured with ELISA.RESULTS: From d 3 to d 14 after Candida albicansgavaging to mice, the number of Candida albicans colonizing in lumen and adhering to mucosal membrane was sharply reduced.Candida albicans translocation to mesenteric lymph nodes occurred at early time points following gavage administration and disappeared at later time points. Meanwhile, the content of specific IgA was increased obviously. Proliferation and differentiation of lymphocytes in lamina propria were also increased.CONCLUSION: Lymphocytes in lamina propria play an important role in intestinal mucosal immunity of specific-pathogen-free mice when they are first inoculated with Candida albicans. The decreasing number of Candida albicans in intestine is related to the increased level of specific IgA antibodies in the intestinal mucus.

关键词：肠移植白色念珠菌粘膜损害淋巴细胞

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Chronic intestinal pseudo-obstruction

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World Journal of Gastroenterology 2008年第19期14卷 2953-2961页

作者： Alexandra Antonucci Lucia Fronzoni Laura Cogliandro Rosanna F Cogliandro Carla Caputo Roberto De Giorgio Francesca Pallotti Giovanni Barbara Roberto Corinaldesi Vincenzo Stanghellini Department of Internal Medicine and Gastroenterology University of Bologna

Chronic intestinal pseudo-obstruction (CIPO) is a se- vere digestive syndrome characterized by derangement of gut propulsive motility which resembles mechanical obstruction, in the absence of any obstructive process. Although uncommon in clinical practice, this syndrome represents one of the main causes of intestinal failure and is characterized by high morbidity and mortality. It may be idiopathic or secondary to a variety of diseases. Most cases are sporadic, even though familial forms with either dominant or recessive autosomal inheritance have been described. Based on histological features in- testinal pseudo-obstruction can be classified into three main categories:neuropathies, mesenchymopathies, and myopathies, according on the predominant involvement of enteric neurones, interstitial cells of Cajal or smooth muscle cells, respectively. Treatment of intestinal pseu- do-obstruction involves nutritional, pharmacological and surgical therapies, but it is often unsatisfactory and the long-term outcome is generally poor in the majority of cases.

关键词：慢性假性肠梗阻免疫组织化学小肠检测肠移植

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肠饲营养防止肠缺血损害动物的远处器官损伤和死亡

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国外医学（外科学分册） 2001年第6期28卷 371-371页

作者：钱明富

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