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中性粒细胞循环游离DNA预测创伤后“二次炎症”打击和脓毒症发生

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中国危重病急救医学 2008年第5期20卷 263-263页

作者：包呈梅(编译) 周国勇(编译) 胡森(审校)

中性粒细胞胞外作用（NETs）是一种新发现的先天性免疫反应。NETs由循环游离DNA（cf-DNA）、组蛋白、中性粒细胞胞质衍生蛋白如蛋白酶组成。为了研究cf-DNA／NETs对多发伤后脓毒症的发生发展及病死率的潜在预测作用，德国研究人员对45... 详细信息

中性粒细胞胞外作用（NETs）是一种新发现的先天性免疫反应。NETs由循环游离DNA（cf-DNA）、组蛋白、中性粒细胞胞质衍生蛋白如蛋白酶组成。为了研究cf-DNA／NETs对多发伤后脓毒症的发生发展及病死率的潜在预测作用，德国研究人员对45例多发伤患者（创伤严重度评分〉16分）的血清cf-DNA／NETs进行了检测。

关键词：中性粒细胞游离DNA 细胞循环预测作用脓毒症创伤后先天性免疫反应创伤严重度评分

孕妇先兆子痫时母婴血浆中细胞外循环的DNA浓度升高

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国外医学（妇产科学分册） 2002年第1期29卷 56-56页

作者：张竹梅

有研究表明先兆子痫孕妇血浆中其胎儿循环的DNA总量升高。为证实母体循环中DNA量的变化进行测定。先兆子痫诊断标准为妊娠20周后间隔4 h 2次血压≥18.7/12 kPa(140/90 mmHg),或舒张压≥14.7 kPa(110mmHg),并有蛋白尿≥300 mg/24h,严重... 详细信息

有研究表明先兆子痫孕妇血浆中其胎儿循环的DNA总量升高。为证实母体循环中DNA量的变化进行测定。先兆子痫诊断标准为妊娠20周后间隔4 h 2次血压≥18.7/12 kPa(140/90 mmHg),或舒张压≥14.7 kPa(110mmHg),并有蛋白尿≥300 mg/24h,严重先兆子痫至少有下列标准之一:2次血压>21.3/14.7 kPa(160/110 mmHg),蛋白尿>5g/24h,子痫HELLP综合征,持续右上头痛或胎儿宫内生长<5个百分点;共取先兆子痫孕妇血浆44例,其中12例有严重体征;妊娠期血压正常的53例作对照,均单胎,妊娠男胎39例,

关键词：先兆子痫血浆 DNA浓度细胞循环诊断

基因差异表达的研究技术及其在寄生虫学研究中的应用

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中国人兽共患病杂志 2003年第4期19卷 98-101,105页

作者：苑纯秀蔡幼民冯新港中国农业科学院上海家畜寄生虫病研究所上海200232

高等生物约有10万个不同的基因，其中只有约15％达。基因表达的选择性左右种种生命过程-发育和分化、对刺激的反应、细胞循环的调节、衰老以及细胞程序性死亡。正常的发育以及由疾病引起的病理改变都是基因表达变化的结果，基因表达的... 详细信息

高等生物约有10万个不同的基因，其中只有约15％达。基因表达的选择性左右种种生命过程-发育和分化、对刺激的反应、细胞循环的调节、衰老以及细胞程序性死亡。正常的发育以及由疾病引起的病理改变都是基因表达变化的结果，基因表达的变化可谓是细胞生命活动调控机制的核心。因此，比较细胞在不同生理、病理或不同生长发育阶段的基因表达差异，可以发现与细胞生长分化或致病性相关的基因，为解析生命活动过程，探索疾病发生的分子机理，寻找合适的药物靶标分子，开发有效的疫苗提供极其重要的信息。基因的差异表达有两种方式：a表达基因的种类改变（新出现或消失）；b同一类基因表达量的改变（上调或下调）。传统的基因分析方法主要是单个基因的克隆表达。但生命现象并不是单基因功能的简单堆积，而是高度有组织的多基因的网络效应。因此，研究细胞全基因组在生理和各种病理条件下基因网络的动态变化十分必要。近十几年来，国内外学者摸索出几种在基因组水平上分析基因差异表达的新方法，其中主要有mRNA差异显示PCR法图（differential display of mRNA by meas of the polymerase chaDD-PCR），cDNA代表性差异分析技术（representatiodifference analysis of cDNA-RDA），抑制性消减杂交技术（suppression subtration hybtidization,的系列分析（serialysis of gene expresNA微阵列技术（cDNA microarray).这些已广泛应用于生命科学的许多领域，本文现就以上几种技术的基本原理，过程,主要优缺点及其在寄生虫学研究中的应用做一简要综述.

关键词：基因表达研究技术寄生虫学研究细胞循环细胞程序性死亡

过氧化物酶体激活物活化受体γ活化对人胃癌细胞周期的影响(英文)

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The Chinese-German Journal of Clinical Oncology 2008年第9期7卷 534-537页

作者： Jun Shi Aiguo Meng Chunyan Liu Clinical Laboratory Affiliated Hospital of North China Coal Medical College Tangshan 063000 China Department of Pharmaceutics North China Coal Medical College Tangshan 063000 China

Objective: To investigate the effect of activation of peroxisome proliferator-activated receptor gamma (PPARγ) on cell cycle arrest of gastric carcinoma cell line MGC803. Methods: The inhibitory of pioglitazone (PGZ) on proliferation of MGC803 cells was analyzed by MTT assay. Cell cycle was detected by flow cytometry (FCM). The expressions of PPARγ, cyclin D1 and cell cycle protein-dependent kinase CDK4 in MGC803 cells were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Results: Treatment with 0.1-10 μmol/L PGZ for 96 h significantly inhibited cell proliferation. The proportion of MGC803 cells at G1 phase was significantly increased when treated with 10 μmol/L PGZ for 48, 72 and 96 h, and showed an apparent G1 phase arrest. The expression of PPARγ was at a low level in MGC803 cells and significantly up-regulated when treated with 10 μmol/L PGZ for 48 h (P < 0.01). The expression of CDK4 in MGC803 cells was remarkably down-regulated when treated with 10 μmol/L PGZ for 48 h and the expression of cyclin D1 was slightly down-regulated (P < 0.01). Conclusion: Activation of PPARγ significantly induced G1 phase arrest, which was associated with down-regulation of the expressions of CDK4 and cyclin D1.

关键词：激活效应 PPARy 胃癌细胞循环临床研究

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一类三维生化系统的定性分析

云南大学学报(自然科学版) 1993年第3期 236-241页

作者：刘正荣李继彬云南省应用数学研究所云南省应用数学研究所云南大学数学系

本文用定性的方法讨论了一类7参数三维生化系统的动力学性质,给出了参数空间的划分,研究了平衡点的存在性、唯一性及稳定性。最后还讨论了该类系统周期解的存在性,从而证明了细胞循环在某些条件下是呈周期性的。

关键词：细胞循环存在性稳定性

Role of Ran GTPase in cell cycle regulation

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Chinese Science Bulletin 2004年第6期49卷 535-541页

作者： JIANGQing LUZhigang ZHANGChuanmao DepartmentofCellBiologyandGenetics.NationalKeyLaboratoryofBiomembraneandMembraneBiotechnology CollegeofLifeSciencesPekingUniversityBeijing100871China

Ran, a member of the Ras GTPase superfamily, is a multifunctional protein and abundant in the nucleus. Many evidences suggest that Ran and its interacting proteins are involved in multiple aspects of the cell cycle regulation. So far it has been conformed that Ran and its interacting proteins control the nucleocytoplasmic transport, the nuclear envelope (NE) assembly, the DNA replication and the spindle assembly, although many details of the mechanisms are waiting for elucidation. It has also been implicated that Ran and its interacting proteins are involved in regulating the integrity of the nuclear structure, the mRNA transcription and splicing, and the RNA transport from the nucleus to the cytoplasm. In this review we mainly discuss the mechanisms by which Ran and its interacting proteins regulate NE as-sembly, DNA replication and spindle assembly.

关键词： Ran GTPase 细胞循环 DNA复制纺锤体集成细胞核细胞膜 G蛋白

Regulation of c-Jun/JunB heterodimers mediated by Epstein-Barr virus encoded latent membrane protein 1 on p16

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Chinese Science Bulletin 2004年第7期49卷 676-683页

作者： SONGXin AIMidan CHENXiaoxi DENGXiyun TAOYongguang GONGJianping WUQiao CancerResearchInstitute XiangyaSchoolofMedicine.CentralSouthUniversityChangsha410078China MolecularMedicalCenter TongjiHospitalTongjiMedicalUniversityWuhan430030China KeyLaboratoryoftheMinistryofEducationforCellBiology.andTumorCellEngineering SchoolofLifeSciencesXiamenUniversity.Xiamen361005China

Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP1) is considered as the major oncogenic protein of EBV encoded proteins, which could transactivate many transcription factors including activator protein 1 (AP-1). Transcription factor plays its role in biological effects through binding to the target gene promoter,transactivating the transcription of target gene, regulating the target gene expression, etc. Recently, we found that LMP1 could mediate a new heterodimer form of c-Jun and JunB, which could bind to AP1 DNA sequence. In this report,we confirmed p16 as a putative target gene of c-Jun/JunB using bioinformatics. We used Tet-on-LMP1 HNE2 cell line as a cell model, which is a dual-stable LMP1 integrated HNE2 cell line and the expression of LMP1 could be regu-lated by the Tet system, and we wanted to explore whether c-Jun/JunB heterodimers mediated by LMP1 could regulate p16. Data demonstrated that c-Jun/Jun B heterodimers me-diated by LMP1 downregulated both the promoter activity and p16 expression, and accelerated the cell cycle progression. These findings established a new direct connection between the AP-I singnal pathway and the cell cycle, and provided a new model for the carcinogenesis mechanism.

关键词： c-Jun JunB p16 潜在膜蛋白1 疱疹病毒致癌蛋白基因转录细胞循环 EBV

An integrative model and analysis of cell cycle in fission yeast

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Chinese Science Bulletin 2005年第17期50卷 1896-1903页

作者： TENG Hu HUANG Xun XIU Zhilong FENG Enmin Department of Bioscience and Biotechnology Dalian University of Technology Dalian 116024 China Department of Applied Mathematics Dalian University of Technology Dalian 116024 China

According to the recent investigation on cell cycle of fission yeast, a mathematical dynamic model is for-mulated. Four cyclins, e.g. Puc1, Cig1, Cig2 and Cdc13, are investigated here. The interacting networks between the cy-clins and the process of cell cycle are mathematically de-scribed. The functions of these cyclins are particularly ana-lyzed. Comparison among different mutants indicates that the cyclins play an important role in cell cycle.

关键词：细胞循环裂变酵母数学模型蛋白质交互网络

p15甲基化、cyclin D1在血液病中的研究进展

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中国医学文摘（肿瘤学） 2004年第4期18卷 326-328页

作者：孙英王占聚崔为发山东省潍坊医学院潍坊261042

细胞循环周期是细胞生长增殖的基础,其内源性调控途径为cyclin-CDK-CKI。eyclin D1与p15都是作用于G_1/S交界处,cyclin D1是细胞周期启动因子,与细胞周期呈正相关;p15是一个CKI分子,作为抑癌基因与细胞增殖呈负相关,其失活主要是甲基化... 详细信息

细胞循环周期是细胞生长增殖的基础,其内源性调控途径为cyclin-CDK-CKI。eyclin D1与p15都是作用于G_1/S交界处,cyclin D1是细胞周期启动因子,与细胞周期呈正相关;p15是一个CKI分子,作为抑癌基因与细胞增殖呈负相关,其失活主要是甲基化。两者的失衡则导致细胞周期异常、肿瘤的发生。检测其变化,有助于血液病的诊断、治疗及判断预后。通过对其失活机制的研究,应用甲基化抑制剂等抑制其异常,对血液病的治疗将是一个新的发展方向。

关键词：血液病治疗甲基化细胞周期诊断肿瘤失活机制细胞循环负相关调控途径