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重组细菌活载体疫苗研究进展

中国预防兽医学报 2022年第2期44卷 219-224,231页

作者：陈妍卉刘军亭于潜刘久源裴翊峰庞富生高栋鹏董浩吉林农业大学生命科学学院吉林长春130118 吉林农业大学生物反应器与药物开发教育部工程研究中心/生命科学学院吉林长春130118

目前,动物传染病的防治主要依赖于疫苗,而疫苗主要采用减毒疫苗或灭活疫苗。虽然减毒疫苗和灭活疫苗的研制技术成熟,但仍有较多的不足之处。减毒疫苗是选择具有活性的无毒或毒力较弱的非致病性病原体所制成的疫苗,能够诱导机体产生细胞... 详细信息

目前,动物传染病的防治主要依赖于疫苗,而疫苗主要采用减毒疫苗或灭活疫苗。虽然减毒疫苗和灭活疫苗的研制技术成熟,但仍有较多的不足之处。减毒疫苗是选择具有活性的无毒或毒力较弱的非致病性病原体所制成的疫苗,能够诱导机体产生细胞免疫反应和体液免疫反应,但在接种后可能会出现毒力返强的现象[1]。因此,出于安全考虑,预防疾病一般首选灭活疫苗,但灭活疫苗在灭活后可能出现病毒灭活不全的现象。

关键词：减毒疫苗灭活疫苗细胞免疫反应体液免疫反应活载体疫苗病毒灭活毒力返强预防疾病

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微丝错构介导的小金蝠蛾细胞免疫耐受反应

应用昆虫学报 2021年第6期58卷 1305-1311页

作者：倪若尧李苗苗吴佩佩高欣欣张寰秦启联张继红孟茜中国科学院动物研究所农业虫害鼠害综合治理研究国家重点实验室北京100101 中国科学院大学生命科学学院北京100049

【目的】小金蝠蛾Thitarodes xiaojinensis细胞免疫反应耐受冬虫夏草菌Ophiocordyceps sinensis的侵染,是非常独特的宿主昆虫与病原真菌免疫互作的研究模型。本研究旨在明确冬虫夏草菌侵染对小金蝠蛾血细胞形态以及伪足形成相关基因表... 详细信息

【目的】小金蝠蛾Thitarodes xiaojinensis细胞免疫反应耐受冬虫夏草菌Ophiocordyceps sinensis的侵染,是非常独特的宿主昆虫与病原真菌免疫互作的研究模型。本研究旨在明确冬虫夏草菌侵染对小金蝠蛾血细胞形态以及伪足形成相关基因表达的影响,初步解析宿主细胞免疫反应耐受该真菌侵染的机制。【方法】利用扫描电子显微镜技术和激光共聚焦荧光显微技术,分别观察并比较健康小金蝠蛾和感染冬虫夏草菌的小金蝠蛾血细胞的形态差异。通过实时定量PCR技术检测冬虫夏草菌感染前后,宿主血细胞微丝组装相关基因的表达变化。【结果】冬虫夏草菌侵染后,小金蝠蛾血细胞丝状伪足和片状伪足的形成受到抑制;促微丝组装基因DRF显著下调表达(P细胞微丝的组装受到阻碍,导致血细胞伪足的形成受到抑制,进而使得宿主细胞免疫反应耐受该真菌的侵染。

关键词：冬虫夏草菌小金蝠蛾细胞免疫反应微丝丝状伪足

Vaccination with dendritic cells pulsed with hepatitis C pseudo particles induces specific immune responses in mice

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World Journal of Gastroenterology 2012年第8期18卷 785-793页

作者： Kilian Weigand Franziska Voigt Jens Encke Birgit Hoyler Wolfgang Stremmel Christoph Eisenbach Department of Gastroenterology and Hepatology University Hospital Heidelberg Department of Medicine Johanna-Etienne-Kran-kenhaus Neuss

AIM: To explore dendritic cells (DCs) multiple functions in immune modulation. METHODS: We used bone-marrow derived dendritic cells from BALB/c mice pulsed with pseudo particles from the hepatitis C virus to vaccinate naive BALB/c mice. Hepatitis C virus (HCV) pseudo particles consist of the genotype 1b derived envelope proteins E1 and E2, covering a non-HCV core structure. Thus, not a single epitope, but the whole "viral surface" induces immunogenicity. For vaccination, mature and activated DC were injected subcutaneously twice. RESULTS: Humoral and cellular immune responses measured by enzyme-linked immunosorbent assay and interferon-gamma enzyme-linked immunosorbent spot test showed antibody production as well as T-cellsdirected against HCV. Furthermore, T-cell responses confi rmed two highly immunogenic regions in E1 and E2 outside the hypervariable region 1. CONCLUSION: Our results indicate dendritic cells as a promising vaccination model for HCV infection that should be evaluated further.

关键词：丙型肝炎病毒细胞免疫反应树突状细胞疫苗接种颗粒组成脉冲接种小鼠诱导

Giardia infection:Protein-losing enteropathy in an adult with immunodeficiency

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World Journal of Gastroenterology 2012年第19期18卷 2430-2433页

作者： Alexandre Khodr Furtado Virginia Lucia Ribeiro Cabral Thiago Nunes Santos Eli Mansour Cristiane Kibune Nagasako Sonia Leticia Lorena Rogerio Antunes Pereira-Filho Faculdade de Ciências Médicas Universidade de Campinas-UnicampRua Tessália Vieira de Camargo126Cidade Universitária "Zeferino Vaz"13083-887 CampinasBrazil

The case of a 52-year-old woman with a past history of thymoma resection who presented with chronic diarrhea and generalized edema is the focal point of this article.A diagnosis of Giardia lamblia infection was established,which was complicated by protein-losing enteropathy and severely low serum protein level in a patient with no urinary protein loss and normal liver function.After anti-helmintic treatment,there was recovery from hypoalbuminemia,though immunoglobulins persisted at low serum levels leading to the hypothesis of an immune system disorder.Good's syndrome is a rare cause of immunodeficiency characterized by the association of hypogammaglobulinemia and thymoma.This primary immune disorder may be complicated by severe infectious diarrhea secondary to disabled humoral and cellular immune response.This is the first description in the literature of an adult patient with an immunodeficiency syndrome who presented with protein-losing enteropathy secondary to giardiasis.

关键词：血清蛋白水平感染性腹泻免疫机能贾第虫病免疫球蛋白肠成人细胞免疫反应

Mechanism of T cell regulation by microRNAs

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Cancer Biology & Medicine 2013年第3期10卷 131-137页

作者： Juan Liu Chang-Ping Wu Bin-Feng Lu Jing-Ting Jiang Department of Tumor Biological Treatment The Third Affiliated Hospital of Soochow University Department of Immunology University of Pittsburgh School of Medicine

MicroRNAs(miRNAs) are small,non-coding single-stranded RNAs that can modulate target gene expression at posttranscriptional level and participate in cell proliferation,differentiation,and apoptosis.T cells have important functions in acquired immune response;miRNAs regulate this immune response by targeting the mRNAs of genes involved in T cell development,proliferation,differentiation,and function.For instance,miR-181 family members function in progression by targeting Bcl2 and CD69,among others.MiR-17 to miR-92 clusters function by binding to CREB1,PTEN,and Bim.Considering that the suppression of T cell-mediated immune responses against tumor cells is involved in cancer progression,we should investigate the mechanism by which miRNA regulates T cells to develop new approaches for cancer treatment.

关键词： microRNA 调节性T细胞调控机制细胞免疫反应 miRNA 癌症治疗转录后水平微RNA

自体CIK、DC-CIK与半合子DC-CIK抗肿瘤免疫反应的比较研究(英文)

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Chinese Journal of Cancer 2010年第7期29卷 641-648页

作者：王其京王慧潘科 State Key Laboratory of Oncology in Southern China Department of Experimental Research Sun Yat-sen University Cancer Center

Background and Objective:Cytokine-induced killer(CIK) cells and autologous dendritic cells-CIK(DCCIK) cells co-cultured with autologous dendritic cells(DCs) and CIK cells are commonly used for immunotherapy recently.We compared the anti-tumor immune response of CIK cells,autologous DC-CIK cells,and semi-allogeneic DC-CIK cells to explore a more effective anti-tumor adoptive immunotherapy approach.Met hods:Peripheral monocytes were isolated from patients with renal carcinoma,lung cancer,or maxillary squamous cell carcinoma and their healthy adult children.Isolated cells were cultured and induced as DCs and CIK cells in vitro.CIK cells from patients were co-cultured with autologous DCs and DCs from their children respectively,generating DC-CIK cells and semi-allogeneic DC-CIK cells.The anti-tumor activities of autologous CIK cells,autologous DC-CIK cells,and semi-allogeneic DC-CIK cells were measured by LDH assay.Intracellular staining was used to test the secretion of cytokines.Flow cytometry was applied for detecting the phonotype changes of these three types of cells.Cell proliferation and cell apoptosis were detected by 5,6carboxyfluorescein diacetate succinimidyl ester(CFSE) and Annexin V/PI respectively.Result s:Compared with autologous CIK cells and DC-CIK cells,semi-allogeneic DC-CIK cells significantly enhanced the anti-tumor activity and IFN-γ secretion,reduced IL-4 secretion,increased the ratio of CD3+CD56+ cells and CD3+CD8+ cells,decreased the number of CD4+CD25+ cells,promoted cell proliferation,and lessened cell apoptosis.Conclusions:Semi-allogeneic DC-CIK cells had a stronger anti-tumor effect than did autologous CIK cells and DC-CIK cells.Our results provided experimental evidence for clinical application of DC-CIK cells.

关键词：抗肿瘤活性细胞免疫反应细胞因子树突状细胞体外诱导 CIK DC 自体

结核丸联合含环丝氨酸化疗方案对耐多药肺结核患者肺功能和外周血Th1/Th17细胞免疫反应的影响

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现代生物医学进展 2023年第13期23卷 2499-2502,2459页

作者：雷丽萍裴异杨晓云朱亭亭胡羽萌南华大学附属长沙中心医院结核科湖南长沙410000 南华大学附属南华医院中医科湖南衡阳421002

目的:探讨结核丸联合含环丝氨酸化疗方案对耐多药肺结核(MDR-TB)患者肺功能和外周血辅助性T细胞(Th)1/Th17细胞免疫反应的影响。方法:将我院于2018年5月~2020年3月期间收治的98例MDR-TB患者根据信封抽签法分为对照组(49例,含环丝氨酸化... 详细信息

目的:探讨结核丸联合含环丝氨酸化疗方案对耐多药肺结核(MDR-TB)患者肺功能和外周血辅助性T细胞(Th)1/Th17细胞免疫反应的影响。方法:将我院于2018年5月~2020年3月期间收治的98例MDR-TB患者根据信封抽签法分为对照组(49例,含环丝氨酸化疗方案治疗)和观察组(49例,结核丸联合含环丝氨酸化疗方案治疗)。对比两组痰菌阴转率、病灶吸收率、空洞闭合率、肺功能指标、外周血Th1/Th17细胞免疫反应相关指标和不良反应发生率。结果:观察组的病灶吸收率、痰菌阴转率、空洞闭合率均高于对照组,不良反应总发生率显著低于对照组(P细胞介素(IL)-17低于对照组同期(P细胞免疫反应。

关键词：结核丸含环丝氨酸化疗方案耐多药肺结核肺功能外周血Th1/Th17 细胞免疫反应

太极拳练习对非小细胞肺癌术后生存者细胞免疫的影响——随机对照实验

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Journal of Sport and Health Science 2013年第2期2卷 104-108页

作者： Ya-Jun Zhang Ru Wang Pei-Jie Chen Ding-Hai Yu Department of Sports Medicine School of KinesiologyShanghai University of Sport Department of Wu Shu Shanghai University of Sport

背景:虽有证据显示太极拳练习有助于改善免疫系统功能,但太极拳练习对细胞免疫的影响尚需进一步研究。本研究旨在探讨太极拳练习对非小细胞肺癌患者细胞免疫的影响。方法:本研究中,非小细胞肺癌术后生存者被随机分到太极拳练习组(n=16)... 详细信息

背景:虽有证据显示太极拳练习有助于改善免疫系统功能,但太极拳练习对细胞免疫的影响尚需进一步研究。本研究旨在探讨太极拳练习对非小细胞肺癌患者细胞免疫的影响。方法:本研究中,非小细胞肺癌术后生存者被随机分到太极拳练习组(n=16)和对照组(n=16)。太极拳练习组受试者进行16周太极拳练习。太极拳练习前后检测两组受试者外周血辅助性T淋巴细胞和抑制性T淋巴细胞比率(CD4:CD8)以及淋巴细胞表面补体调节蛋白(CRPs;CD55和CD59)表达量。采用重复测量方差分析完成所有数据处理(n=27)。结果:16周后,与对照组相比,太极拳练习可明显延缓淋巴细胞表面CD55蛋白表达的增加(p细胞表面CD59表达无显著影响。淋巴细胞表面补体调节蛋白表达的改变与T淋巴细胞亚群变化无显著相关。结论:16周太极拳练习对非小细胞肺癌术后生存者辅助性T淋巴细胞和抑制性T淋巴细胞比率无明显影响,但可显著延缓淋巴细胞表面补体调节蛋白CD55表达的升高。

关键词：非小细胞肺癌细胞免疫反应随机分配幸存者太极拳试验计划手术训练

Effect of growth hormone on small intestinal homeostasis relation to cellular mediators IGF-I and IGFBP-3

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World Journal of Gastroenterology 2009年第43期15卷 5418-5424页

作者： Betul Ersoy Kemal Ozbilgin Erhun Kasirga Sevinc Inan Senol Coskun Ibrahim Tuglu Division of Pediatric Endocrinology Department of PediatricsFaculty of MedicineCelal Bayar University Manisa 45010Turkey Department of Histology and Embryology Faculty of MedicineCelal Bayar UniversityManisa 45010 Turkey Division of Pediatric Gastroenterology De- partment of PediatricsFaculty of MedicineCelal Bayar UniversityManisa 45010Turkey Department of Histology and Embryology Faculty of MedicineCelal Bayar University Manisa 45010Turkey Department of Pediatrics Faculty of Medicine Celal Bayar UniversityManisa 45010Turkey

AIM:To evaluate the effects of growth hormone(GH) on the histology of small intestines which might be related to the role of insulin like growth factor(IGF)-I, IGF-binding protein 3(IGFBP-3)and its receptors. METHODS:Twelve week-old adult male Wistar albino rats were divided into two groups.The study group(n =10),received recombinant human growth hormone (rGH)at a dose of 2 mg/kg per day subcutaneously for 14 d and the control group(n=10)received physiologic serum.Paraffin sections of jejunum were stained with periodic acid shift(PAS)and hematoxylin and eosin(HE) for light microscopy.They were also examined for IGF-I, IGFBP-3 and IGF-receptor immunoreactivities.Staining intensity was graded semi-quantitatively using the HS- CORE. RESULTS:Goblet cells and the cells in crypt epitheliawere significantly increased in the study group compared to that of the control group.We have demonstrated an increase of IGF-I and IGFBP-3 immunoreactivities in surface epithelium of the small intestine by GH application.IGF-I receptor immunoreactivities of crypt,villous columnar cells,enteroendocrine cells and muscularis mucosae were also more strongly positive in the study group compared to those of in the control group. CONCLUSION:These findings confirm the important trophic and protective role of GH in the homeostasis of the small intestine.The trophic effect is mediated by an increase in IGF-I synthesis in the small intestine, but the protective effect is not related to IGF-I.

关键词：重组人生长激素 IGFBP 小肠细胞平衡关系胰岛素样生长因子结合蛋白细胞免疫反应生长因子受体调解