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Combined gene therapy of endostatin and interleukin 12 with polyvinylpyrrolidone induces a potent antitumor effect on hepatoma

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World Journal of Gastroenterology 2004年第15期10卷 2195-2200页

作者： Pei-YuanLi Ju-ShengLin Zuo-HuaFeng Yu-FeiHe He-JunZhou XinMa Xiao-KunCai De-AnTian InstituteofLiverDiseases TongjiHospitalTongjiWuhan430030HubeiProvinceChina DepartmentofBiochemistryandMolecularBiology TongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030HubeiProvinceChina

AIM: To study the antitumor effect of combined gene therapy of endostatin and interleukin 12 (IL-12) with polyvinylpyrrolidone (PVP) on mouse transplanted ***: Mouse endostatin eukaryotic plasmid (pSecES) with a mouse Igk signal sequence inside and mouse IL-12 eukaryotic plasmid (pmIL-22) were transfected into BHK-22cells respectively. Endostatin and IL-22 were assayed by ELISA from the supernant and used to culture endothelial cells and spleen lymphocytes individually. Proliferation of the latter was evaluated by M-I-r. H22 cells were inoculated into the leg musde of mouse, which was injected intratumorallywith pSecES/PVP, pmIL-12/PVP or pSecES+pmIL-12/PVPrepeatedly. Tumor weight, serum endostatin and serumIL-22 were assayed. Tumor infiltrating lymphocytes, tumormicrovessel density and apoptosis of tumor cells were also displayed by HE staining, CD32 staining and ***: Endostatin and IL-12 were secreted after transfection, which could inhibit the proliferation of endothelial cells or promote the proliferation of spleen *** growth was highly inhibited by 92.8% after injection of pSecES+pmIL-i2/PVP accompanied by higher serum endostatin and IL-22, more infiltrating lymphocytes, fewertumor vessels and more apoptosis cells compared with injection of pSecES/PVP, pmIL-i2/PVP or vector/***: Mouse endostatin gene and IL-12 gene can be expressed after intratumoral injection with *** of hepatoma can be inhibited synergisticly,lymphocytes can be activated to infiltrate, and tumor cells are induced to apoptosis. Hepatoma can be highly inhibited or eradiated.

关键词：组合基因白细胞间介素12 聚乙烯吡咯烷酮 IL-12 感应抗癌作用肝细胞癌肿瘤 PVP

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