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Dependence of the E. coli promoter strength and physical parameters upon the nucleotide sequence

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2005年第11期6卷 1063-1068页

作者： BEREZHNOY AndreyY. SHCKORBATOV Yuriy G. National Scientific Center Kharkov Physical-Technical Institute Kharkov 61108 UkraineInstitute of Biology Kharkov National University Kharkov 61077 Ukraine

The energy of interaction between complementary nucleotides in promoter sequences of E. coli was calculated and visualized. The graphic method for presentation of energy properties of promoter sequences was elaborated on. Data obtained indicated that energy distribution through the length of promoter sequence results in picture with minima at –35, –8 and +7 regions corresponding to areas with elevated AT (adenine-thymine) content. The most important difference from the random sequences area is related to –8. Four promoter groups and their energy properties were revealed. The promoters with minimal and maximal energy of interaction between complementary nucleotides have low strengths, the strongest promoters correspond to promoter clusters characterized by intermediate energy values.

关键词：核苷序列 DNA 脱氧核糖核酸分子结构随机序列

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Emergence of a new satellite RNA from cucumber mosaic virus isolate P1

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Journal of Zhejiang University Science 2003年第3期4卷 336-339页

作者： SandraPérezAlvarez 薛朝阳周雪平 InstituteofBiotechnology ZhejiangUniversityHangzhou310029China

The cucumber mosaic virus (CMV) isolate P1 caused very mild symptoms on many plant *** serial passages by mechanical inoculation over five years, CMV P1 caused severe symptoms on several tobacco cultivars and tomato. A specific band of approximately 0.3 kb in length was amplified by RT-PCR with primers synthesized based on reported CMV satellite RNA (satRNA) sequences. Sequence analysis showed there were two satRNAs (Sat-Pl-1 and Sat-P1-2). Sat-Pl-1 contained 335 nucleotides, and Sat-P1-2 contained 394 nucleotides. These two satRNAs shared 64% overall nucleotide sequence homology, and differences between the two satRNAs included mutations as well as deletions. Sat-Pl-1 was identical to a satRNA (Z96099) reported in 1995 in CMV P1. Based on differences in the sequence and secondary structure between these two satRNAs, we conclude that Sat-P1-2 represents the emergence of a new satellite ( necrotic satellite) from attenuated satRNA populations. The possible effect of the emergence of this new satRNA is discussed.

关键词：黄瓜花叶病病毒 P1 RNA卫星核苷序列

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