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Reporter-based screen for Arabidopsis mutants compromised in nonhost resistance

Chinese Science Bulletin 2008年第7期53卷 1027-1034页

作者： CHEN HuaMin PAN JunSong ZHAO XiuXiang ZHOU JianMin CAI Run School of Agriculture and Biology Shanghai Jiaotong University Shanghai 200240 China National Institute of Biological Sciences Beijing 102206 China College of Plant Protection Shenyang Agricultural University Shenyang 110161 China

Plants are exposed to many potentially pathogenic microbes in the environment, but each species is only susceptible to a limited number of pathogens. The broad resistance is referred to as nonhost re-sistance. To date, little is known about the underlying mechanism of nonhost resistance and the sig-naling transduction process. Here we describe a simple method for isolating Arabidopsis nonhost re-sistance mutants against a nonadapted bacterial pathogen. A RAP2.6 promoter-driven LUC reporter system was developed to replace the tedious bacterial growth assay during the primary screening. The RAP2.6-LUC reporter gene is normally induced by the virulent bacterium Pseudomonas syringae pv tomato but not the nonadapted bacterium P. syringae pv phaseolicola. By using this method we iso-lated 4 mutants displaying strong reporter activity in response to P. syringae pv phaseolicola, which were characterized in some details. ebs1, ebs2, ebs3, and ebs4 (enhanced bacterial susceptibility) were compromised in resistance against P. syringae pv phaseolicola and/or P. syringae pv tomato. In addi-tion, ebs4 showed enhanced hypersensitive response to the incompatible bacterium P. syringae pv tomato (avrB). These results demonstrated that the method is suited for large scale screening for nonhost resistance mutants.

关键词：突变体抗寄生植物电阻基因

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