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Clinical Significance of Telomerase Activity and Human Telomerase Reverse Transcriptase mRNA Expression for Differential Diagnosis of Malignant and Benign Liver Lesions

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Chinese Journal of Clinical Oncology 2004年第6期1卷 442-447页

作者： RuifangFan WeifengWong XinxinBu XianlingGuo FengqiJia ZhengyouLi MengchaoWu LixinWei TumorImmunologyandGeneTherapyCenter EasternHepatobiliarySurgeryHospitaltheSecondMilitaryMedicalUniversityShanghai200438China. DepartmentofHepatobiliarySurgery LanzhouGeneralHospitalofLanzhouMilitaryRegionLanzhou730050China.

OBJECTIVE To study the clinical significance of telomerase activity and human telomerase reverse transcriptase (hTERT) mRNA expression for differential diagnosis of malignant and benign liver *** Telomerase activity was determined by an ELISA-based telomeric repeat amplification protocol (EUSA-TRAP) assay on 130 surgical resected liver samples and 58 percutaneous biopsied liver samples. In addition, the samples were assayed for expression of hTERT mRNA measured by a. reverse transcriptase-polymerase chain reaction (RT-PCR). Postoperative pathological examinations were also performed on these *** Among the 130 surgical liver samples, the positive rates of telomerase activity in hepatocettutar carcinoma (HCC), liver cirrhosis and chronic hepatitis tissues were 85.9% (55/64), 25.0% (8/32) and 8.3% (2/24) respectively, and the positive rates of hTERT mRNA expression were 89.1% (57/64), 25.0% (8/32) and 8.3% (2/24) respectively, Neither telomerase activity nor hTERT mRNA expression was detected in 10 normal liver *** the 58 biopsied liver specimens, the positive rates of telomerase activity in HCC, cholangiocellular carcinoma, focal nodular hyperplasia, inflammatory pseudotumor and adenomatous hyperplasia tissues were 85.7% (30/35), 100% (4/4), 33.3% (4/12), 25.0% (1/4) and 33.3% (1/3) respectively, and the positive rates of hTERT mRNA expression were 88.6% (31/35), 100% (4/4), 33.3% (4/12), 25.0% (1/4) and 33.3% (1/3) respectively. The positive level of telomerase activity and hTERT mRNA expression in malignant fiver tumors was sign(ficantly higher than that found in benign liver lesions (P<0.01 ).CONCLUSION Determination of telomerase activity or hTERT mRNA expression in percutaneous biopsied liver tissues may be useful for differential diagnosis in malignant and benign liver lesions.

关键词：临床作用未端酶活动性转录酶基因表达微分诊断恶性良性肝损害肿瘤

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Differential Diagnoses of Solitary Pulmonary Nodules in Patients with an Extrcrlhoracic Malignant Tumor：CT and Pathologic Correlations

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Chinese Journal of Clinical Oncology 2005年第2期2卷 575-582页

作者： ChengzhouLi XiangshengXiao ShiyuanLiu HuiminLi WeihuaDong BiaoDing QinghuaZhang DepartmentofRadiology theSixthHospitalofShanghaiJiaoTongUniversityShanghai200233China DepartmentofRadiology ChangzhengHospitaloftheSecondMilitaryMedicalUniversityShanghai200003China

OBJECTIVE To determine the possibility of definitive diagnosis for solitary pulmonary nodules in patients with a primary extrathoracic malignant neoplasm (ETM-SPN), and to further evaluate the value of CT for differential diagnosis in ETM-SPN by a multivariate retrospective *** Eighty-three patients with pathologically and clinically proven ETM-SPN with a diameter smaller than 3cm were included in this *** pathological characteristics of the SPN were correlated with those of the extrathoracic neoplasm, with the patient's age, gender, smoking history, disease-free time interval between the diagnosis of the extrathoracic malignancy and that of the lung lesion. In all 83 cases, CT scans were reviewed to confirm the solitary nature, size, and nodular morphology of the lung *** Of all 83 cases, the mean age was (57.43±15.34) years. There were 51 males and 32 females, with the ratio of 1.59:1. The lesions included solitary metastasis in 43 cases, pulmonary malignant lesions in 33, and benign lesions in seven. Between the primary lung cancers and solitary metastasis groups, there was no significant difference in the gender ratio (1.20:1 vs 2.31:1, x^2=0.0209, P>0.05), but there was a significant difference between the mean age (62.48±11.96 years vs 54.10±16.49 years, t=3.34, P0.05). Of 81 cases who were followed-up, the mean time of the disease-free interval between extrapulmonary malignancy diagnosis and pulmonary lesion differentiation was 39.73±6.29 months (range 0-300 months, median 20.00 months), whereas those in the primary lung cancer group and metastatic group were 65.62±13.45 months and 22.83±4.19 months respectively. This differe

关键词：微分诊断独立性肺结节胸腔外肿瘤 CT检查病理学

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