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Survivin isoforms and clinicopathological characteristics in colorectal adenocarcinomas using real-time qPCR

World Journal of Gastroenterology 2011年第12期17卷 1614-1621页

作者： Anastasia Pavlidou Maria Dalamaga Christos Kroupis George Konstantoudakis Maria Belimezi George Athanasas Kleanthi Dimas Department of Clinical Biochemistry Attikon University HospitalAthens 12462Greece 4th University Surgical Clinic Attikon University HospitalAthens 12462Greece

AIM:To investigate three isoforms of survivin in colorectal ***:We used the LightCycler Technology(Roche),along with a common forward primer and reverse primers specific for the splice variants and two common hybridization probes labeled with fluorescein and LightCycler-Red fluorophore(LC-Red 640).Real time quantitative polymerase chain reaction(PCR) was performed on cDNAs from 52 tumor specimens from colorectal cancer patients and 10 unrelated normal colorectal *** the patients group,carcinoembryonic antigen(CEA) and CA19-9 tumor markers were also measured ***:Wild type survivin mRNA isoform was expressed in 48%of the 52 tumor samples,survivin-2b in 38%and survivin-ΔΕx3 in 29%,while no expression was found in normal *** mRNA expression of wild type survivin presented a significant correlation with the expression of the ratio of survivin-2b,survivin-ΔΕx3,survivin-2b/wild type survivin and survivin-ΔΕx3/wild type survivin(P<0.001).The mRNA expression of wildsurvivin and survivin-ΔΕx3 was related with tumor size and invasion(P=0.006 and P<0.005,respectively).A significant difference was found between survivin-2b and morphologic cancer ***,the ratio of survivin-ΔEx3/ wild-survivin was significantly associated with *** association was observed between the three isoforms and grade,metastasis,Dukes stage and *** three isoforms were not correlated with CEA and ***:Survivin isoforms may play a role in cell apoptosis and their quantification could provide information about clinical management of patients suffering from colorectal cancer.

关键词： Survivin mRNA isoforms Apoptosis 基因 Colorectal 腺癌实时量的聚合酶链反应 Lightcycler

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