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世界核心医学期刊文摘（神经病学分册） 2005年第10期1卷 50-51页

作者： Kraybill M.L. Larson E.B. Tsuang D.W. M.M. Cherrier 张殿增 S-116 MIRECC VAPSHCS 1660 S. Columbian Way Seattle WA 98108 United States Dr.

Objective: To examine the neuropsychological profile of dementia patients fro m a community- based autopsy sample of dementia, comparing Alzheimer disease (A D), Lewy body pathology (LBP) alone, and LBP with coexistent AD (AD/LBP). Methods: The authors reviewed 135 subjects from a community- based study of dementia for wh om autopsy and brain tissue was available. Diagnostic groups were determined acc ording to standard neuropathologic methods and criteria, and the presence of LBs was determined using α - synuclein immunostaining. Neuropathologically define d diagnostic groups of AD, AD/LBP, and LBP were examined for differences on neur opsychological test performance at the time of initial study enrollment. Results : There were 48 patients with AD alone, 65 with LB and AD pathology (AD/LBP), an d 22 with LBP alone (LBP alone). There were no significant differences between g roups demographically or on performance of enrollment Mini- Mental State Examin ation (MMSE) or Dementia Rating Scale (DRS). AD patients performed worse than th e LBP patients on memory measures (Fuld Object Memory Evaluation Delayed Recall, Wechsler Memory Scale Logical Memory Immediate and Delayed Recall; p < 0.05) an d a naming task (Consortium to Establish a Registry for Alzheimer’ s Disease Na ming; p < 0.05). LBP patients were more impaired than AD patients on executive f unction (Trail Making Test Part B; p < 0.05) and attention tasks (Wechsler Adult Intelligence Scale- Revised Digit Span; p < 0.05). Decline in MMSE and DRS sco res over time were greatest in the patients with AD/LBP. Conclusions: In a commu nity- based sample of older, medically complicated patients with dementia, ther e are neuropsychological differences between dementia subtypes at the time of di agnosis. In particular, patients with Alzheimer disease (AD) alone and AD/Lewy b ody pathology (LBP) had more severe memory impairment than patients with LBP. LB P alone was associated with more severe executive dysfunction. Patients with AD/ LBP had the most rapid rate of c

关键词：阿尔茨海默病 Lewy体认知功能神经心理学记忆评估神经病理学记忆力损伤执行功能记忆量表免疫染色

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皮肤鳞状细胞癌及癌前病变中环氧化酶-2的表达及血管形成研究:35例免疫组化对照研究

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世界核心医学期刊文摘（皮肤病学分册） 2005年第3期1卷 54-55页

作者： Nijsten T. Colpaert C.G. Vermeulen P.B. 党倩丽 Department of Pathology University of Antwerp Antwerp Belgium Dr.

Background: Cyclooxygenase (COX)- 2 expression and tumour-induced angiogen esis appear to be increased in squamous cell carcinoma (SCC) of the skin. In oth er cancers, COX- 2 is a pro-angiogenic factor. The association between angiog enesis and COX- 2 has not been studied in skin cancer. Objectives: To assess th e onset of increased COX- 2 expression and angiogenesis in the multistage carcinogenesis of SCC as well as the correlation between those two parameters. Patients/methods: We performed a retrospective paired immunohistoch emical analysis of normal skin, actinic keratosis (AK), Bowen’s disease (BD) a nd SCC among 35 individuals. Specimens were considered COX- 2 immunopositive wh en 5% or more of the tumour cells showed clear evidence of immunostaining. To quantify active angiogenesis, we used a Ki- 67- CD34 double-labelling immuno histochemical stain and calculated the fraction of proliferating endothelial cel ls. The Chalkley method was used to determine the microvessel density. To detect hypoxia, a carboanhydrase IX immunostain was used. Results: Compared with norma l epidermis (0% ), AK (31% ), BD (22% ) and SCC (40% )- were significantly more likely to be COX- 2 immunopositive (P < 0.01). The fraction of proliferat ing endothelial cells and the Chalkley scores paralleled multistage carcinogenes is (P < 0- 05 between different stages). COX- 2 immunopositivity was fairly co rrelatedwith hypoxia and higher proliferating endothelial cell fractions but not with Chalkley counts. Conclusions: Induction of COX- 2 expression and angiogen esis are both early events in the development of SCC. In addition to ultraviolet light,hypoxia and COX- 2 may be involved in skin tumour angiogenesis.

关键词：皮肤鳞状细胞癌癌前病变环氧化酶免疫染色博温病光化性角化病有显著性差异统计学结果上皮细胞组织缺氧

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微波在免疫组织化学中的应用

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微波学报 1996年第3期12卷 228-233,174页

作者：陈浩季天仁电子科技大学高能所成都610054

本文综述微波技术在免疫组织化学中的应用现状及其进展,着重介绍了微波在组织固定、抗原恢复和免疫染色方面的应用,并对微波免疫组织化学的应用前景进行了展望.

关键词：免疫组织化学抗原恢复免疫染色微波

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子宫腺肌病患者IL-8受体的表达

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世界核心医学期刊文摘（妇产科学分册） 2006年第6期2卷 36-37页

作者： Ulukus M. Ulukus E.C. Seval Y. A.Arici 张旸 Section of Reproductive Endocrinology and Infertility Department of Obstetrics and Gynecology Yale University School of Medicine New Haven CT 06520- 8063 United StatesDr.

Objective: To investigate the expression of interleukin- 8 (IL- 8) receptors CXCR1 and CXCR2 in adenomyosis. Design: Comparative immunohistochemical study. Setting: Academic medical center. Patient(s): Thirty women who had undergone hysterectomy and were proved histopathologically to have adenomyosis, and 27 women without adenomyosis who had a hysterectomy for nonendometrial pathology such as leiomyomata or benign ovarian cysts. Intervention(s): Tissue sections were immunostained with murine monoclonal anti- human CXCR1 and CXCR2 antibodies. Main Outcome Measure(s): Microscopic evaluation to assess the presence and localization of CXCR1 and CXCR2 throughout the menstrual cycle in both eutopic endometrial and adenomyotic tissues of women with adenomyosis and compare it with normal endometrium. Result(s): In eutopic endometrium of women with adenomyosis, proliferative phase samples showed higher epithelial CXCR1 and CXCR2 staining intensity compared with proliferative phase samples of normal endometrium. Adenomyosis foci expressed higher epithelial CXCR1 compared with the homologous eutopic endometrium and normal endometrium. On the other hand, adenomyosis foci and the homologous eutopic endometrium showed similar epithelial CXCR2 staining intensity, and this expression was higher than the normal controls. Conclusion(s): Intrinsic abnormalities concerning IL- 8 and its receptor system may be present in the eutopic endometrium of women affected by adenomyosis. These findings suggest that IL- 8 receptors may be involved in the pathogenesis and/or pathophysiology of adenomyosis.

关键词：子宫腺肌病 IL-8受体女性患者子宫内膜病变正常子宫内膜 CXCR2 子宫切除术免疫组化研究良性卵巢囊肿免疫染色

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超声引导高负压细针抽吸组织以评估实质性病变:一项先导性研究

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世界核心医学期刊文摘（胃肠病学分册） 2006年第3期2卷 44-45页

作者： Larghi A. Noffsinger A. Dye C.E. I. Waxman 郝筱倩 University of Chicago Section of Gastroenterology MC 9028 5758 S. Maryland Ave Chicago IL 60637 United States.Dr

Background: The capability of obtaining tissue samples for histologic examination during EUS has theoretical advantages over cytology alone. The objective was to evaluate the feasibility and the yield of EUS-guided FNA tissue acquisition (EUS-FNTA) by using high negative pressure suction. Methods: The study design is a prospective, observational pilot study set at a tertiary referral center. Twenty-seven patients with a solid mass amenable to sampling with EUS were included in the study. FNA with a 22-gauge needle was used for a total of 5 passes. An additional pass with the same needle was performed by applying continuous high negative pressure suction using the Alliance II inflation system. The main outcome measurements were the rate of tissue acquisition and the diagnostic accuracy of EUS-FNTA. Observations: Tissue samples were obtained in 26 of the 27 patients (96%). Malignancy was detected in 20 of the 26 biopsy specimens obtained by FNTA and in 20 of the 27 FNA specimens. In 3 patients, EUS-FNTA failed to disclose malignancy, which in two of the patients was diagnosed by FNA. Conversely, EUS-FNTA diagnosed a recurrent malignant thymoma and a schwannoma in two FNA-negative patients. In 3 patients with both FNTA and FNA negative for malignancy, a definitive diagnosis could not be established. Overall, diagnostic accuracy was 76.9%for both EUS-FNTA and EUS-FNA. When combined, a correct diagnosis was achieved in 84.6%of the patients. Immunostaining of the retrieved tissue allowed characterization of the primary tumor in 5 cases and the diagnosis of a schwannoma and two neuroendocrine tumors. Limitations of the study were small sample size and a pilot study. Conclusions: EUS-FNTA has a high yield for the retrieval of core tissue samples. Further studies in which EUS-FNTA is performed before FNA and with variable number of passes are needed to better define its diagnostic role and performance characteristics.

关键词：实质性病变免疫染色神经鞘瘤负压抽吸诊断作用细胞学鉴定试点研究原发性肿瘤测定指标研究

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1例结膜T细胞淋巴瘤的临床病理报道

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世界核心医学期刊文摘（眼科学分册） 2006年第8期2卷 58-59页

作者： Al-Muammar A. Hodge W.G. Farmer J. 张磊 University of Ottawa Eye Institute 501 Smyth Road Ottawa Ont. K1H 8L6 Canada Dr.

Objective: To report a patient with conjunctival T-cell lymphoma, an extremely rare entity. Design: Single case report. Methods: Based on clinical examination, an excisional biopsy and immunostaining were performed on the conjunctival lesion. For management, we excised and performed triple freeze-thaw cryotherapy to the involved area, and we consulted the oncology service. Main Outcome Measures: T-cell and B-cell markers, and clinical examination of the lesion. Results: Both examination and laboratory assessment revealed no evidence of systemic involvement. Conjunctival biopsy showed expansion of the substantia propria with an infiltrate of chronic inflammatory cells (including lymphocytes, plasma cells, and eosinophils), and prominent lymphocyte exocytosis with reactive epithelial changes. The CD- 45 RO (T-cell marker) was strongly positive, whereas the CD- 20 (B-cell marker) was negative. The T-cell receptor gene rearrangement was positive with beta clonality, confirming the diagnosis of T-cell lymphoma. Conclusions: T-cell lymphoma is a rare but possible diagnosis of gelatinous conjunctival lesions. The oncology consultants were reluctant to treat the patient with systemic chemotherapy or radiation because extraconjunctival extension could never be documented. The answer to the question of what is the most appropriate treatment for conjunctival T-cell lymphoma remains unknown.

关键词： T细胞淋巴瘤结膜病冷冻治疗局部活检局部病胞外分泌免疫染色医生会诊基因重组外扩散

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人类恶性黑色素瘤中内皮一氧化氮合酶和血管内皮生长因子的表达及其与血管形成的关系

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世界核心医学期刊文摘（皮肤病学分册） 2006年第5期2卷 39-40页

作者：吴佳纹

Background. Angiogenesis is the major and key factor for growth and invasion of tumours, including malignant melanoma (MM), but the factors that contribute to tumour angiogenesis are still unclear. Objective. To study expression of endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor in human MM and their relation to angiogenesis. To investigate the correlation between eNOS and VEGF and the role of nitric oxide (NO) generated by eNOS in the process of mediating angiogenesis by VEGF. Methods. Tissue sections from 31 patients with MM were examined using immunohistochemistry and morphological quantitative analysis for protein expression of eNOS and VEGF.Microvessel density (MVD) was counted in endothelial cells in immunostained by anti-FVIII:RAg antibody. Results. Positive eNOS and VEGF immunostaining were observed in 77.4% and 83.9% of MM lesions, respectively, whereas pigmented naevi never expessed eNOS and VEGF. A positive correlation between eNOS and VEGF in MM was observed. Expression of eNOS and VEGF was positively correlated with MVD expression in MM, and MVD expression in MM was stronger than in pigmented naevi. Expression of eNOS and VEGF was not correlated with lymph node metastasis. Conclusions. On the basis of the current data showing that malignant melanocytic tumours displayed strong VEGF and eNOS expression, whereas benign melanocytic proliferations showed no immunoreactivity for VEGF and eNOS, such expression may be used as a discriminating factor to distinguish malignant melanoma from pigmented naevi. Expression of eNOS and VEGF may contribute to angiogenesis of MM, eNOS probably plays an important role in mediating VEGF induced angiogenesis.

关键词：恶性黑色素瘤色素细胞瘤色素痣内皮细胞免疫组化法黑色素细胞定量分析法氧化亚氮免疫染色

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原发性子宫内膜鳞状细胞癌:采用免疫组化和分子技术研究的病例报道

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世界核心医学期刊文摘（妇产科学分册） 2005年第7期1卷 47-48页

作者： Giordano G. D' Adda T. Merisio C. Gnetti L. 侯巍 Department of Pathology and Medicine Laboratory Section of Pathology Parma University Viale A. Gramsci 14 43100 Parma Italy

Background. In this paper, we report a case of primary squamous cell carcinoma of the endometrium (PSCCE) with immunohistochemical and molecular study to evaluate the phenotype and to define the etiopathogenesis of this tumor. Case history. A 72- year- old woman was admitted to the Department of Obstetric and Gynecology for weight loss and pelvic mass. Abdominal ultrasonography disclosed the abdominopelvic mass with solid, cystic, and calcified areas. The patient underwent exploratory laparotomy. Intraoperative findings showed an enlarged uterus with perforation of its wall. The surface of omentum was covered with small white nodules. Pathological examination showed features of PSCCE. Immunohistochemical analysis with antibodies for estrogen and progesterone receptors disclosed negativity of neoplastic elements. Immunostaining with p53 tumor- suppressor protein showed the mutation of p53 tumor- suppressor protein as a strong nuclear positivity. Molecular study by polymerase chain reaction (PCR) amplification of tumor DNA did not show any signal for human papilloma virus (HPV) DNA. Conclusion. In summary, unlike the example reported in the literature by others, in the present case we demonstrated that PSCCE is not due to HPV infection, but probably to other pathogenetic mechanisms, which cause a mutation of p53 tumor- suppressor gene. Thus, it is reasonable to conclude that both HPV infection and unclear carcinogenic factors, responsible of p53 tumor- suppressor gene mutation, may cause PSCCE.

关键词：子宫内膜鳞状细胞癌分子技术雌激素受体孕激素受体人乳头瘤病毒肿瘤抑制蛋白子宫壁病理检查免疫染色

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血管内皮生长因子在浆液性卵巢癌中的表达:与拓扑异构酶Ⅱα及疾病预后的关系

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世界核心医学期刊文摘（妇产科学分册） 2005年第2期1卷 43-44页

作者： Brustmann H. 张剑萍

This study investigated the express ion of vascular en-dothelial growth factor(VEGF)and topoisomerase IIα(TPIIα)in a cohort of serous ovarian carcinomas by im-munohistochemistry with regard to o utcome and clinico-pathologic variables.Formalin -fixed,paraffin -embed-ded archival tissue sections of 10benign serous cystade-nomas and 41serous ovarian carcinom as were immunos-tained with antibodies to VEGF and TP IIα.Immunostain-ing for VEGF was scored with regard to quantity and in-tensity of positively stained cells as weak,focal strong,and diffuse strong.TPIIαlabeling indices(LI )were quanti-tated as the percentage of positively stained nuclei in 1000tumor cells.VEGF expression was wea k or negative in serous cystadenomas.In contrast,f ocal or diffuse strong immunostaining was observed in 21cases(51%)of serous carcinomas.VEGF immunoreac tivity was positively related with TPIIαLI (P =0.0055),adverse outcome (P =0.0052),high International Federation of Gynecol-ogy and Obstetrics(FIGO)stage(P =0.0158),and high tumor grade(P =0.0303).TPIIαLI increased with FIGO stage(P =0.0076)as well as tumor grade(P =0.0034)and indicated poor prognosis(P =0.0182).VEGF immunoreactivity is related wi th TPIIαLI and prognostically relevant.Expression of VEGF protein and TPIIαmay serve as markers of aggressive tu mor behavior in serous adenocarcinomas.

关键词：拓扑异构酶Ⅱ 疾病预后染色细胞核染色阳性细胞标记指数免疫反应性免疫染色临床病理学肿瘤侵袭

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重度子宫内膜异位症患者的在位内膜环氧化酶-2表达高于对照组

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世界核心医学期刊文摘（妇产科学分册） 2005年第4期1卷 42-43页

作者： Matsuzaki S. Canis M. Pouly J.-L. 张剑萍

To identify any relationship between cyclooxygenase-2 expression and the intensity of severe, endometriosisrelated dysmenorrhea. Prospective study. University hospital. Patients with deep endometriosis. During surgery, paired samples of tissue representing deep endometriosis and eutopic endometrium were obtained from 46 patients. Control endometrial tissue samples were obtained from 34 fertile women who underwent laparoscopic tubal ligation or reversal of tubal sterilization. Pain assessment for dysmenorrhea was done with a 10-point linear analogue scale. The percentage of surface immunostained for Cox-2 was determined by an immunohistochemical technique. Relationships between pain score for dysmenorrhea and Cox-2 expression were analyzed. Cox-2 expression was significantly higher in eutopic endometrial stromal cells from patients with deep endometriosis than in stroma from controls during the early, mid, and late secretory phases. In endometriosis patients, Cox-2 expression in eutopic endometrial stromal cells was significantly higher in women with more severe dysmenorrhea (pain score ≥7 vs. < 7) during early and mid secretory phases. Elevated Cox-2 expression in stromal cells in eutopic endometrium from patients with deep endometriosis may play a role in severe, endometriosisrelated dysmenorrhea.

关键词：子宫内膜异位症在位内膜环氧化酶痛经程度子宫内膜基质再通术免疫染色输卵管结扎术育龄期大学医院

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