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东北农业大学学报 2005年第2期36卷 170-173页

作者：李桂琴袁强东北农业大学生命科学学院黑龙江哈尔滨150030

解剖学研究表明,三种茄科作物非光合器官(宿存萼和果皮)均具有与叶相似的光合作用结构体系,并具有光合作用功能,三者因存在部位和器官不同,其结构亦不同对应。发育后期,叶绿体破坏、瓦解,并失去光合作用的功能。

关键词：辣椒番茄酸浆光合体系解剖

Effects of Exogenous Nitric Oxide on Photochemical Activity of Photosystem Ⅱ in Potato Leaf Tissue Under Non-stress Condition

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Acta Botanica Sinica 2004年第9期46卷 1009-1014页

作者： YANGJia-Ding ZHAOHa-Lin ZHANGTong-Hui YUNJian-Fei ColdandAridRegionsEnvironmentalandEngineeringResearchInstitute TheChineseAcademyofSciencesLanzhou730000China

Under non-stress condition, effects of exogenous nitric oxide (NO) on chlorophyll fluorescence parameters in detached leaves and leaf discs of potato (Solanum tuberosum L.) were surveyed. Results showed that the maximal quantum efficiency (Fv/Fm) and the effective quantum efficiency (ΦPSⅡ)of photosystem Ⅱ (PSⅡ) were reduced by exogenous NO under illumination (150μmol.m^-2·s^-1, 25℃). This influence was related not only to the concentration of sodium nitroprusside (SNP, a NO donor) solution, but also to the active duration of NO on leaf tissue. Results with leaf discs showed that the effects of SNP on ΦPSⅡ could be prevented by bovine hemoglobin (a powerful NO scavenger), while amixture of NO2^- and NO3^-(the decomposition product of NO or its donor SNP) had much less influence on ΦPSⅡ than SNP, indicating that effects of exogenous SNP on PSⅡ photochemical activity was mainly due to NO generation. Under light (150μmol·m^-2·s^-1, 25℃) for 4h or longer period, the non-photochemical quenching (NPQ) in SNP-soaked leaves was statistically similar to that in H2O-soaked control, but ΦPSⅡ and the proportion of open reaction centers (measured as qP） were lower than control, respectively. After 25min dark-adaptation, the maximal fluorescence (Fm) in SNP treatment (8 and 12h illumination duration) was significantly lower than the control, while the initial fluorescence (Fo) in SNP and H2O-treated leaves had no significant difference. Therefore this indicated that under the present experimental condition, the NO-affected site might not be the PSⅡ reaction centers. On the donor side of PSⅡ, NO putatively influenced the light-harvesting capacity of leaves under light; on the acceptor side, NO-affected sites were some components of electron transport chain after QA, *** enhanced the reductive degree of reaction centers through blocking the electron transport after QA, thus reducing the photochemical activitv of PSⅡ.

关键词：光化学活性光合体系马铃薯叶组织一氧化氮

水生植物有翅星蕨(Microsorum pteropus)对镉的超富集能力及抗性生理研究

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中国科学：生命科学 2017年第10期47卷 1113-1123页

作者：兰心宇王军军阎蕴运李新元徐福留北京大学城市与环境学院地表过程分析与模拟教育部重点实验室北京100871

超富集植物能耐受高浓度的重金属并将其储存于植物各组织中,对于重金属污染环境的修复具有重要意义.本文以沉水植物有翅星蕨(Microsorum pteropus)为研究对象,设置了低浓度镉暴露组(0,0.1,1,5,10,20 mg/L)和高浓度镉暴露组(0.20,40,60,8... 详细信息

超富集植物能耐受高浓度的重金属并将其储存于植物各组织中,对于重金属污染环境的修复具有重要意义.本文以沉水植物有翅星蕨(Microsorum pteropus)为研究对象,设置了低浓度镉暴露组(0,0.1,1,5,10,20 mg/L)和高浓度镉暴露组(0.20,40,60,80,100 mg/L),暴露7天,发现有翅星蕨各组织镉富集浓度的大小顺序为:不定根>羽片>叶柄>根状茎,在0.1mg/L镉暴露情况下,不定根的最大生物富集系数达920.21.各暴露组有翅星蕨对培养液中Cd的去除效率为39.12%~53.99%.当镉暴露浓度为60mg/L时,不定根和羽片中镉的富集浓度分别达到了(3738.39±348.03)及(10652.53±2477.74)mg/kg,具有极强的镉富集能力.同时,各暴露组有翅星蕨的叶绿素、丙二醛、过氧化物酶与超氧化物歧化酶活性、以及叶绿素荧光参数等生理生化和光合作用指标相较对照组并未出现显著的变化.研究表明,有翅星蕨对水体中的Cd具有超强的富集能力,可用于镉污染水体的修复.

关键词：有翅星蕨镉超富集抗氧化系统光合体系

Change of proton motive force across thylakoid membrane in soybean leaf during state transitions

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Chinese Science Bulletin 2003年第7期48卷 652-657页

作者： SU Jihu & SHEN Yungang Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032, China 1. Shanghai Institute of Plant Physiology Chinese Academy of Sciences Shanghai 200032 China

Change of proton gradient across thylakoid membrane in soybean leaves was studied with millisecond delayed light emission (ms-DLE) during the course of state transitions which were indicated by the chlorophyll fluores-cence at room temperature and 77 K. When dark-adapted leaves were induced to stateⅠ with far-red light, Fm/Fo, F685/F735 and the intensity of fast phase of ms-DLE were af-fected slightly. However, during the induction to stateⅡ with red light, both Fm/Fo and F685/F735 decreased immedi-ately and the former were quicker than the latter. In this interval, the intensity of fast phase of ms-DLE increased to a maximum and then decreased to a lower value during the transition to stateⅡ. Nigericin, an uncoupler which elimi-nates the proton gradient across thylakoid membrane, inhib-ited the increase in the intensity of fast phase of ms-DLE during the transition to stateⅡ. Another uncoupler, valino-mycin, which eliminates the membrane potential, did not affect the changes of the intensity of fast phase. These results suggest that the prompt increase in the intensity of fast phase of ms-DLE at the beginning of transitions to stateⅡ is cor-related mainly with the proton gradient released from water oxidation in photosystemⅡ.

关键词：大豆叶状态转换类囊体膜质子运动力量毫秒滞后光发射光合体系

Hydrogen ions directly regulating the oligomerization state of Photosystem I in intact Spirulina platensis cells

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Chinese Science Bulletin 2008年第2期53卷 317-320页

作者： ZHANG Rui XIE Jie HU ChangChao ZHAO JingQuan Beijing National Laboratory for Molecular Sciences (BNLMS) and Key Laboratory of Photochemistry of CAS Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China

H+ concentration induced-monomerization or trimerization of photosystem I (PSI) in cyanobacteria has never been directly observed. In this work, taking characteristic spectra for the trimers and monomers as the indicators, it was experimentally demonstrated that H+ could induce the oligomeric changes of PSI reaction centers in the intact Spirulina platensis cells and also in the isolated thylakoid membrane complexes. Especially, the higher concentration of H+ would induce the monomerization while the lower the trimerization, suggesting the electrostatic interaction should be mainly responsible for changes in the oligomeric state of PSI in Spirulina platensis.

关键词：光合体系三聚作用氢离子植物细胞低聚反应

Low pH-induced conformational changes in 33 kD protein of photosystem Ⅱ

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Chinese Science Bulletin 2004年第9期49卷 921-925页

作者： WENGJun TANCuiyan YUYong RUANKangcheng XUChunhe InstituteofPlantPhysiology ShanghaiInstitutesforBiologicalSci-encesChineseAcademyofSciencesShanghai200032China LaboratoryofProteomics InstituteofBiochemistryandCellBiology.ShanghaiInstitutesforBiologicalSciencesChineseAcademyofSci-encesShanghai200031China

kD protein, located on the lumen side of thylakoid membranes, is one of three extrinsic proteins of photosystemⅡ(PSⅡ). Previous study showed that NBS modification of W241, the only tryptophan in 33 kD protein, is helpful for understanding the function of W241 in main-taining functional conformation of 33 kD protein. In this paper, studies of both circular dichroism and fluorescence spectra showed that upon decreasing pH from 6.2 to 2.5, the conformation of soluble 33 kD protein changed significantly, with an increase or a decrease in percentage of random coil or a-helix and turns. The changes in secondary structures of this protein are pH reversible. After NBS modification at pH 2.5, the conformational change of 33 kD protein was kept fixed. The CD ellipticity at 200 nm for NBS-modified 33 kD protein is much lower than that for control, indicating that the unfolding degree of 33 kD protein was enhanced after the NBS modification. Moreover, the conformational flexibility is lost in NBS-modified 33 kD protein, and the conformational change becomes pH irreversible, indicating that NBS modi-fication blocked the reversibility of conformational change of 33 kD protein. The specific binding capability of NBS-modi- fied 33 kD protein is much lower than that of low pH-treated control. Furthermore, the rebinding of modified protein on PSⅡ membranes cannot restore the activity of oxygen evo-lution. We suggest that it is low pH but not NBS modification of W241 that leads to the conformational change of 33 kD protein from one functional to another non-functional state. The significant capability of proton transport of 33 kD pro-tein is discussed.

关键词： NBS修正 33KD蛋白色氨酸功能性构象类囊体光合体系

ApcD is required for state transition but not involved in blue-light induced quenching in the cyanobacterium Anabaena ***7120

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Chinese Science Bulletin 2008年第21期53卷 3422-3424页

作者： DONG ChunXia ZHAO JinDong State Key Lab of Protein and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 China

Pbycobilisomes(PBS) are able to transfer absorbed energy to photosystem I and Ⅱ,and the distribution of light energy between two photosystems is regulated by state *** this study we show that energy transfer from PBS to photosystem I(PSI) requires *** were unable to perform state transitions in the absence of *** apcD mutant grows more slowly in light mainly absorbed by PBS,indicating that ApcD-dependent energy transfer to PSI is required for optimal growth under this *** apcD mutant showed normal blue-light induced quenching,suggesting that ApcD is not required for this process and state transitions are independent of blue-light induced *** nitrogen fixing condition,the growth rates of the wild type and the mutant were the same,indicating that energy transfer from PBS to PSI in heterocysts was not required for nitrogen fixation.

关键词：蓝藻细菌光合体系藻胆蛋白体状态转移

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手机将用上菠菜电池把太阳光能转化成电能

通信业与经济市场 2005年第5期 61-62页

用菠菜中的“光合体系一号”把太阳光能转化成电能——

关键词：光能转化电能太阳菠菜电池手机光合体系

Mapping Out the Structure of a Spinach Light-Harvester

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Bulletin of the Chinese Academy of Sciences 2004年第3期18卷 130-131页

The major light-harvesting complex of photosystem Ⅱ(LHC-Ⅱ) serves as the principal solar energy collector in the photosynthesis of green plants and presumably also functions in photoprotection under high-light conditions. Many of its structures were revealed by a work of a 3.2A electron crystallographic model of LHC-Ⅱ in 1994. Since then researchers have eagerly awaited a higher-resolution structure to extend knowledge of the light harvesting and energy transfer processes involved in photosynthesis to the level of full atomic data.

关键词：图形结构菠菜光收割机光合体系光合作用模拟研究能量收集

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美科学家解答光合作用之谜

种业导刊 2012年第8期 39-39页

伦斯勒理工大学的生物化学太阳能“巴鲁克60”研究中心的科研人员近日对植物和细菌中太阳能转化为化学能的有效反应各个步骤的细节进行了深入研究。该研究提供了光合作用光合体系II的重要信息，对直接观察光合体系II中太阳能水分解反应... 详细信息

伦斯勒理工大学的生物化学太阳能“巴鲁克60”研究中心的科研人员近日对植物和细菌中太阳能转化为化学能的有效反应各个步骤的细节进行了深入研究。该研究提供了光合作用光合体系II的重要信息，对直接观察光合体系II中太阳能水分解反应的关键问题进行了解答。研究人员对在光合体系II中发生的植物将太阳能转化为化学能的光化学反应的前两个阶段进行了研究，检测了光合体系II的催化部位中基质水分子的绑定和激活过程。

关键词：光合作用解答科学家光合体系分解反应光化学反应太阳能科研人员