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Cypovirus capsid protein VP5 has nucleoside triphosphatase activity

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Virologica Sinica 2017年第4期32卷 328-330页

作者： Jie Yang Qi Qian Teng—Feng Li Xueli Yang Sok Jin W on Xi Zho State Key Laboratory of Virology College of Life Sciences W uhan UniversityWuhan 430072China

As a major protein of cypovirus capsid shell, VP5 per-forms as the clamp protein to stabilize the capsid shellstructure （Yu et al., 2008）. And as a part of viral RNA（vRNA） replication machinery, VP5 has been found topossess an ATP-independent RNA chaperoning activity（Yang et al., 2014）. Here, we report that VP5 also has thenucleoside triphosphatase （NTPase） activity, which canhydrolyze all kinds of NTPs and dNTPs. Thus, ourfinding reveals that VP5 is a multifunctional protein thatmay be involved in diverse processes during cypovirallife cycle.

关键词：中国病毒学英文版

Reconciling individual and population levels of porcine reproductive and respiratory syndrome virus evolution

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Virologica Sinica 2017年第4期32卷 342-345页

作者： Giovanni Franzo Claudia Maria Tucciarone Mattia Cecchinato Michele Drigo Department of Animal Medicine Production and Health(MAPS)University of PaduaViale dell’Università1635020LegnaroItaly

Porcine reproductive and respiratory syndrome virus（PRRSV）, a member of the family Arteriviridae, repre-sents one of the most challenging pathogens in the swineindustry, with serious economic impact. Unfortunately,despite widespread application, vaccination has not beensuccessful in effectively controlling the virus, mainlybecause of the limited cross-protection among differentstrains （Kimman et al., 2009）.

关键词：中国病毒学英文版

A convenient immunochromatographic test strip for rapid detection of Scylla serrata reovirus

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中国病毒学：英文版 2017年第4期32卷 335-337页

作者： Dongyang Fan Jingwen Liu Miaomiao Xu Yangyang Yuan Jifang Yang Jigang Chen College ofFood and Bioengineering Jimei UniversityXiamen 361021China

Scylla serrata reovirus （SsRV）, first discovered in Chinain 2004, is now known to be widespread in the mud crab（Scylla serrata） farming pond of China. （Chen et al.,2008）. Since there are no effective treatments ... 详细信息

关键词：中国病毒学英文版

A practical approach to generate suitable de novo synthesis RNA template for a flavivirus RNA-dependent RNA polymerase

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Virologica Sinica 2017年第4期32卷 331-334页

作者： Wei Shi Peng Gong Key Laboratory of Special Pathogens and Biosafety W uhan Institute of VirologyChinese Academy of SciencesW uhan 430071.China University of Chinese Academy of Sciences Beijing l 00049China

Due to its high transcription efficiency, bacteriophage T7RNA polymerase （T7 RNAP） has long been utilized inbacterial and in vitro systems to generate large quantityof RNA for various purposes （Studier and Moffatt,1986）. However, heterogeneity at the 3＇-end of the RNAtranscript remains a major limitation for in vitro RNApreparation using T7 RNAP. When approaching the endof its DNA template, T7 RNAP tends to add a few extranucleotides not directed by the template strand sequence,resulting in a mixture of RNA products that are equal toor longer than the desired length （Milligan et al., 1987）.

关键词：中国病毒学英文版

INSTRUCTIONS FOR AUTHORS

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Virologica Sinica 2017年第4期32卷 I0001-I0001页

Research articles belong to the full length article type, and should in-clude elements as follows： title, running title, and byline; correspondentfootnote; abstract and keywords; introduction; materials and methods;results; discussion; acknowledgements; compliance; author contribu-tions; references; figure legends and figures/tables. The article lengthgenerally should not exceed 6,000 words, and detailed requirements arein the Format section.

关键词：中国病毒学英文版