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Preparation of Immobilized ε-polylysine PET Nonwoven Fabrics and Antibacterial Activity Evaluation

Journal of Wuhan University of Technology(Materials Science) 2011年第4期26卷 675-680页

作者：郝丽梅杨荆泉 Institute of Medical Equipment the Academy of Military Medical Sciences National Bioprotection Engineering Center

A novel antibacterial material （L-PET） was prepared by immobilizing ε-polylysine on polyethylene terephthalate （PET） nonwoven fabrics. Surface modifications of the fabric were performed by using a chemical modification procedure where carboxyl groups were prepared on the PET surface, a coupling agent was grafted, and the ε-polylysine was immobilized. Scanning electron microscopy （SEM） was used to analyze the surface morphology of the fabrics, while the toluidine blue method and X-ray photoelectron spectroscopy （XPS） were used to evaluate the grafting densities. The antibacterial activities of the L-PET were investigated by using the shaking-flask method. The electron micrographs showed that the surface of the blank PET and the modified fabrics did not change. The results of XPS analysis confirmed that ε-polylysine was successfully grafted onto the surface of PET. The results of the antibacterial experiments showed that L-PET fabrics had excellent antibacterial activity against Escherichia coli and Staphylococcus aureus, and that L-PET fabrics were stable in storage for at least two years.

关键词： PET nonwoven fabric ε-polylysine immobilization antibacterial activity antibacterial material

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Enhanced production of cytidine 5'-monophosphate using biocatalysis of di-enzymes immobilized on amino-functionalized sepharose

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Chinese Journal of Chemical Engineering 2023年第6期58卷 40-52页

作者： Xiaohong Zhou Wenfeng Zhou Wei Zhuang Chenjie Zhu Hanjie Ying Hongman Zhang School of Chemistry and Molecular Engineering Nanjing Tech UniversityNanjing 211816China State Key Laboratory of Materials-Oriented Chemical Engineering College of Biotechnology and Pharmaceutical EngineeringNational Engineering Technique Research Center for BiotechnologyNanjing Tech UniversityNanjing 211816China

Cytidine 5'-monophosphate(5'-CMP)is an essential nucleotide for *** this study,enhanced production of 5'-CMP was realized by the transformation of cytidine using co-immobilized di-enzymes,uridine-cytidine kinase(UCK)and acetate kinase(AcK).The immobilization yield of the enzyme had a clear correlation with the surface charges as zeta potential(ξ).Among them,ε-polylysinefunctionalized sepharose(SA-EPL,ξ=9.31 m V)showed high immobilization yield(78.8%),which was4.9-fold than that of nitrilotriacetic acid functionalized sepharose(SA-NTA,ξ=-12.6 m V).The residual activity of affinity co-immobilized enzyme(EPL-Ni/EPL@Ac K-UCK)was higher than 70.6%after recycled 10 ***,this study provides an effective approach for the production of 5'-CMP with the advantages of low adenosine 5'-triphosphate(ATP)consumption,reduced side reactions,and improved reusability by co-immobilized UCK and Ac K on the functionalized Sepharose.

关键词： Sepharose ε-polylysine Dual-enzyme cascade Cytidine 5'-monophosphate Enzyme immobilization

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A targeted nanoplatform co-delivery of pooled siRNA and doxorubicin for reversing of multidrug resistance in breast cancer

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Nano Research 2022年第7期15卷 6306-6314页

作者： Hongmei Liu Ding Ma Jinpeng Chen Li Ye Yiping Li Yuexia Xie Xue Zhao Hanbing Zou Xiaojing Chen Jun Pu Peifeng Liu State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer InstituteRenji HospitalShanghai Jiao Tong University School of MedicineShanghai 200032China Central Laboratory Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of Biliary-Pancreatic Surgery Shanghai Jiao Tong University School of Medicine Affiliated Renji HospitalShanghai 200127China Thyroid Breast Surgery Nantong First People’s HospitalNantong 226001China Shanghai University of Traditional Chinese Medicine Shanghai 200032China

Multi-drug resistance(MDR)has become the largest obstacle to the success of cancer patients receiving traditional chemotherapeutics or novel targeted ***,we developed a targeted nanoplatform based on biodegradable boronic acid modifiedε-polylysine to co-deliver P-gp siRNA,Bcl-2 siRNA,and doxorubicin for overcoming the *** targeted nanoplatform showed a robust suppressing efficiency for the invasion,proliferation,and colony formation of adriamycin(ADR)resistant breast cancer cell line(MCF-7/ADR)cells in *** ATP responsiveness of the nanoplatform was also proved in the *** the in vivo antitumor experiment,the targeted nanoplatform showed a significant inhibition of tumor growth with good *** goal of this study is to develop a novel and facile strategy to prepare a highly efficient and safe gene and drug delivery system for MDR breast cancer based on biocompatibleε-polylysine polymers.

关键词： multi-drug resistance(MDR) breast cancer adenosine triphosphate(ATP)responsiveness ε-polylysine

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