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Kinetic analysis of γ-glutamyltransferase reaction process for measuring activity via an integration strategy at low concentrations of γ-glutamyl p-nitroaniline

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第3期12卷 180-188页

作者： Zhi-rong LI Yin LIU Xiao-lan YANG Jun PU Bei-zhong LIU Yong-hua YUAN Yan-ling XIE Fei LIAO Key Laboratory of Medical Laboratory Diagnostics of Ministry of Education Chongqing Medical University Chongqing 400016 China Unit for Biotransformation and Protem Blotechnology Chongqing Key Laboratory of Bioehemlcal and Molecular Pharmacology Chongqing Medical University Chongqing 400016 China

At 0.12 mmol/L γ-glutamyl p-nitroaniline(GGPNA),an improved integrated method was developed for kinetic analysis of γ-glutamyltransferase(GGT) reaction process and the integration with the classical initial rate method to measure serum *** the improved integrated method,an integrated rate equation,which used the predictor variable of reaction time and considered inhibitions by both GGPNA and products,was nonlinearly fit to GGT reaction *** the integration strategy,classical initial rates were estimated when GGPNA consumption percentages were below 50%;otherwise,maximal reaction rates of GGT were estimated by the improved integrated method and converted into initial rates according to the differential rate equation at 0.11 mmol/L *** inte-gration strategy was validated using optimized GGT kinetic parameters and 10-s intervals to record reaction curves within 8.0 *** the integration strategy,there was a linear response from 0.9 to 32.0 U/L GGT,coefficients of variation were below 3.5%for GGT from 8.0 to 32.0 U/L(n=5) ,and GGT activities in clinical sera responded linearly to their classical initial rates at 2.00 mmol/L GGPNA with an expected ***,the integration strategy was successful in measuring GGT at 0.12 mmol/L GGPNA.

关键词： Integration strategy Chromogenic substrate Data processing γ-glutamyltransferase Kinetic analysis Serum enzyme assay

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Model based on γ-glutamyltransferase and alkaline phosphatase for hepatocellular carcinoma prognosis

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World Journal of Gastroenterology 2014年第31期20卷 10944-10952页

作者： Xin-Sen Xu Yong Wan Si-Dong Song Wei Chen Run-Chen Miao Yan-Yan Zhou Ling-Qiang Zhang Kai Qu Si-Nan Liu Yue-Lang Zhang Ya-Feng Dong Chang Liu Department of Hepatobiliary Surgery the First Affiliated Hospital of Medical College Xi’an Jiaotong University Department of Radiology the First Affiliated Hospital of Medical College Xi’an Jiaotong University Department of Obstetrics and Gynecology University of Kansas School of Medicine

AIM: To determine the prognostic value of alkaline phosphatase(ALP) and γ-glutamyltransferase(GGT) for hepatocellular carcinoma(HCC).METHODS: We analyzed the outcome of 172 HCC patients who underwent liver resection. Receiver operating characteristic(ROC) curve analysis was performed to determine the cut-off value of ALP and GGT. Then, preoperative risk factors for survival were evaluated by multivariate analysis. Based on the significant factors, aprognostic score model was ***: By ROC curve analysis, ALP > 120 U/L and GGT > 115 U/L were considered elevated. Overall survival(OS) and tumor-free survival(TFS) for patients with elevated ALP and GGT were significantly worse than for patients with ALP and GGT within the normal range. Multivariate analysis showed that the elevated levels of ALP, GGT and tumor size were independent prognostic factors. Giving each positive factor as a score of 1, we established a preoperative prognostic score model. The 5-year OS for patients with a score of 0, 1, 2 and 3 were 84.0%, 45.9%, 44.1% and 0%, respectively, while the TFS was 80.6%, 40.0%, 38.8% and 0%, respectively. When combining patients with scores of 1 and 2 into the middle risk group, and patients with scores of 0 and 3 into the low-risk and highrisk groups, respectively, different outcomes would be significantly distinguished by the risk ***: Elevated ALP and GGT levels were risk predictors in HCC patients. Our prognostic model might vary the outcomes of patients from different risk groups.

关键词： Alkaline phosphatase γ-glutamyltransferase Prognos

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Serological diagnostic factors for liver metastasis in patients with colorectal cancer

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World Journal of Gastroenterology 2010年第32期16卷 4084-4088页

作者： Xiong-Zhi Wu Feng Ma Xue-Lin Wang Department of Zhong Shan-Men Inpatient Cancer Institute and Hospital Tianjin Medical University Tianjin 300171China

AIM:To investigate the serological diagnostic factors for liver metastasis in patients with colorectal ***:One hundred and six adult in-patients with colorectal cancer were studied and divided into patients with liver metastasis(n = 56) and patients without liver metastasis(n = 50).Serum levels of tumor and biochemical markers for liver were measured at the time of ***:The mean survival time was 55.9 mo,36.8 mo and 68.3 mo for the overall patients,patients with liver metastasis and patients without liver metastasis,*** dehydrogenase(LDH) level was significantly correlated with the survival time of colorectal cancer *** levels of alanine aminotransferase,aspartate aminotransferase,γ-glutamyltransferase(GGT),LDH and carcinoembryonic antigen(CEA) were significantly higher in patients with liver metastasis than in those without liver *** with lymph node metastasis had a higher risk of liver metastasis than those without lymph node *** cut points of LDH,GGT and CEA for screening liver metastasis were 180 U/L,30 U/L and 5.0 μg/L,*** sensitivity was 64.3%,69.6% and 70.4%,and the specificity was 64.0%,60.0% and 52.4%,*** sensitivity of parallel test was 85.2% for LDH and CEA,and 92.6% for GGT and CEA,*** specificity of serial test was 85.7% for LDH(or GGT) and ***:Early diagnosis of liver metastasis is of great *** sensitivity and specificity of combined tumor and biochemical markers are rather good in screening colorectal liver metastasis.

关键词： Liver metastasis Colorectal cancer Lactate dehydrogenase γ-glutamyltransferase Carcinoembryonic antigen

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Carbohydrate antigen 19-9 as a novel prognostic biomarker in distal cholangiocarcinoma

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World Journal of Gastrointestinal Surgery 2021年第9期13卷 1025-1038页

作者： Tao Jiang Shao-Cheng Lyu Lin Zhou Jing Wang Han Li Qiang He Ren Lang Department of Hepatobiliary Surgery Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China

BACKGROUND Distal cholangiocarcinoma(DCC)presents as one of the relatively rare malignant tumors in the digestive system and has a poor long-term *** resection is currently the most appropriate therapy for patients with DCC because of the lack of effective adjuvant ***,it is important to accurately predict the prognosis for formulating a reasonable treatment plan and avoiding unnecessary surgical *** To minimize the interference of obstructive jaundice on carbohydrate antigen 19-9(CA19-9)level by adapting CA19-9 toγ-glutamyltransferase(GGT)as an indicator,to determine the strong associations between CA19-9/GGT and postoperative neoplasm recurrence and long-term outcome of *** We enrolled 186 patients who were diagnosed with DCC between January 2010 and December 2019 and performed radical excision with strict criteria as follows in our *** operating characteristic curves were drawn according to preoperative CA19-9/GGT and 1-year *** on this,patients were divided into two groups(group 1,low-ratio,n=81;group 2,high-ratio,n=105).Afterwards,by the way of univariate and multivariate analysis,the risk factors influencing postoperative tumor recrudesce and long-term prognosis of patients with DCC were screened *** Optimum cut-off value of CA19-9/GGT was *** in group 2 represented higher CA19-9 and lymphatic metastasis rate accompanied by lower GGT,when compared with group 1(P<0.05).The 1-,3-and 5-year overall survival rates of patients in groups 1 and 2 were 88.3%,59.2%and 48.1%,and 61.0%,13.6%and 13.6%,respectively(P=0.000).Multivariate analysis indicated that CA19-9/GGT,lymphatic metastasis and tumor differentiation were independent risk factors for tumor recurrence and long-term prognosis of *** Elevation of CA19-9/GGT performed better as a biomarker of aggressive carcinoma and predictor of poor clinical outcomes by reducing the effect of obstruction of biliary tract on CA

关键词： Distal cholangiocarcinoma Pancreaticoduodenectomy Carbohydrate antigen 19-9 γ-glutamyltransferase Relapse Prognosis

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Monitoring the level of serum paraoxonase 1 activity in liver transplantation patients

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Hepatobiliary & Pancreatic Diseases International 2005年第2期4卷 178-181页

作者： Gen-Yun Xu, Guo-Cai Lv, Yu Chen, Yong-Chuan Hua, Shen-Mei Zhu and Yi-Da Yang Hangzhou, China Clinical Laboratory, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Clinical Laboratory First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China. genyunxu@***

BACKGROUND: Paraoxonase 1(PON1) is an ester hydro- lase in serum and in the liver. Studies have suggested that PON1 measurement to the current battery of tests may im- prove the evaluation of chronic liver diseases. The aim of this study was to investigate the clinical significance of mo- nitoring the level of serum PON1 activity in liver transplan- tation patients. METHODS: A series of biochemical indexes were moni- tored in preoperative, operative and postoperative serum samples of 17 liver-transplanted patients. The change of se- rum PON1 level and its relations with other biochemical in- dexes were analyzed. RESULTS: PON1 was distributed normally in the healthy population and its reference value ranged from 45.5 to 265.8 U/mL. The PON1 level of all patients was lower than that of control group significantly (P<0.001); the level be- gan to elevate continuously 5 minutes after opening of the portal vein and was higher than that 90 minutes after open- ing of the portal vein ( P <0.05). Two days after operation it was still higher than the normal. The levels of serum ALT and AST elevated more significantly after opening of the portal vein than before operation and they were higher than the normal values till 2 days after the operation. CONCLUSIONS: The level of PON1 in serum may be taken as one of the effective indexes to assess whether the implant is alive and to monitor liver function of the patient together with other tests.

关键词： paraoxonase 1 liver transplantation alanine transaminase aspartate transaminase γ-glutamyltransferase cholinesterase

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