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Analysis of LMW-GS,α-and γ-gliadin Gene Coding Sequences from Triticum macha

Agricultural Sciences in China 2010年第2期9卷 163-169页

作者： XIONG Li-juan WANG Ji-rui ZHENG You-liang Triticeae Research Institute Sichuan Agricultural University Ya＇an 625014 P.R.China Key Laboratory of Southwestern Crop Germplasm Utilization Ministry of Agriculture Ya＇an 625014 P.R.China

Novel LMW-GS （low molecular weight glutenin subunit）,α- and γ-gliadin from Triticum macha accessions were characterized via genomic PCR, which can do favor to improve the wheat quality. The complete coding regions of two α-gliadin, two γ-gliadin and two LMW-GS gene sequences, which designed as Gli-Mal, Gli-Ma2, Gli-Mrl, Gli-Mr2, Glu-LM1 and Glu-LM2, encoded the mature proteins with 307, 241, 348, 302, 474 and 377 amino acid residues, respectively. Gli-Mal and Gli-Ma2 were recognized as pseudogenes due to the in-frame stop codons. The amino acid sequences deduced from these gene sequences were characterized with the typical structure of α- or γ-gliadin or LMW- m type proteins with the exception of Gli-Ma2. Phylogenetic analysis showed Gli-Mal was closely related to those from T. aestivum, whereas Gli-Ma2 seemed to be more homologous with the gene sequences from Dasypyrum breviaristatum. Gli-Mrl was closely related to those from T. turgidum ssp. dicoccoides, while Gli-Mr2 was the nearest to those from T. aestivum. Glu-LM1 was closely related to those from Aegilops tauschii, whereas GIu-LM2 seemed to be more homologous with those from T. durum.

关键词： T. macha α-gliadin γ-gliadin LMW-GS gene clone sequence analysis

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Cloning and characterization of novel γ-gliadin genes from Aegilops markgrafii in relation to evolution and wheat breeding

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The Crop Journal 2017年第4期5卷 290-295页

作者： Min Li Xuye Du Xin Ma Lingrang Kong Heze University Heze 274000 Shandong China State Key Laboratory of Crop Biology College of Agronomy Shandong Agricultural University Tai'an 271018 Shandong China College of Life Science Guizhou Normal University Guiyang 550025 Guizhou China

gliadins are the major components of storage proteins in wheat and play an important role in determining the extensibility properties of *** the present work,six novel full-length γ-gliadin genes were cloned from the C genome of Aegilops markgrafii using a PCR-based *** of the deduced amino acid sequences showed that the cloned genes had primary structures that were similar,but not identical,to published γ-gliadins from other wheat-related *** lengths of the open reading frames(ORFs)ranged from 909 to 963 bp,and the repetitive and glutamine-rich domains were mainly responsible for the size of the *** extra cysteine residue was present in the repetitive domain of sequence *** amino acid sequences of γ-gliadin genes from *** were searched for the five peptides identified as T cell stimulatory epitopes in celiac disease(CD)*** Gliγ-3 was present in sequences JX566513 and *** Gliγ-5 was present only in *** otherγ-gliadins contained no toxic *** results provide information to better understand the use of *** in wheat breeding and the evolutionary relationship of theγ-gliadin genes in *** and other Triticeae species.

关键词： γ-gliadin Evolution Celiac disease Flour quality

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Identification of a Group of Novel γ-gliadin Genes

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Journal of Integrative Agriculture 2014年第2期13卷 290-298页

作者： QI Peng-fei WEI Yu-ming Ouellet Thérèse CHEN Qing WANG Zhao WEI Zhen-zhen ZHENG You-liang Triticeae Research Institute Sichuan Agricultural University Agriculture & Agri-Food Canada Eastern Cereal and Oilseed Research CentreOttawa K1A 0C6Canada

γ-gliadins are an important component of wheat seed storage proteins. Four novel γ-gliadin genes （Gli-ngl to Gli-ng4） were cloned from wheat （Triticum aestivum） and Aegilops species. The novel γ-gliadins were much smaller in molecular size when compared to the typical γ-gliadins, which was caused by deletion of the non-repetitive domain, glutamine-rich region, 3＂ part of the repetitive domain, and 5＇ part of the C-terminal, possibly due to illegitimate recombination between the repetitive domain and the C-terminal. As a result, Gli-ngl and Gli-ng4 only contained two and three cysteine residues, respectively. Gli-ngl, as the representative of novel γ-gliadin genes, has been sub-cloned into an Escherichia coli expression system. SDS- PAGE indicated that the both cysteine residues of Gli-ngl could participate in the formation of intermolecular disulphide bonds in vitro. Successful cloning of Gli-ngl from seed cDNA of T. aestivum cv. Chinese Spring suggested that these novel γ-gliadin genes were normally transcribed during the development of seeds. Phylogenic analysis indicated that the four novel γ-gliadin genes had a closer relationship with those from the B （S） genome of wheat.

关键词： γ-gliadin cysteine disulphide bond illegitimate recombination

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