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Influence of CXCR4/SDF-1 axis on E-cadherin/β-catenin complex expression in HT29 colon cancer cells

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World Journal of Gastroenterology 2011年第5期17卷 625-632页

作者： Lin Wang Cui-Ling Li Lei Wang Wen-Bin Yu Hai-Peng Yin Guang-Yong Zhang Li-Feng Zhang Sheng Li San-Yuan Hu Department of General Surgery Qilu Hospital Shandong University Jinan 250012 ShandongProvince China Key Laboratory for Modem Medi-cine and Technology of Shandong Province Institute of BasicMedicines Shandong Academy of Medical Sciences Jinan250062 Shandong Province China Depatment of Hepatobiliary Surgery Shandong Tu-mor Hospital Jinan 250117 Shandong Province China

AIM： To study the influence of CXCR4/stromal cell- derived factor-1 （SDF-1） axis on E-cadherin/β-catenin complex expression in HT29 colon cancer ceils and its underlying mechanisms. METHODS： Effect of SDF-1 on E-cadherin/β-catenin expression was detected by immunocytochemistry. E-cadherin and/3-catenin mRNA expression levels were measured by reverse transcriptase-polymerase chain reaction. SDF-l-induced phosphorylation of phosphati- dylinositol 3-kinase （PI3K）/AKT and β-catenin was detected by Western blotting. RESULTS： The E-cadherin and β-catenin mRNA ex-pression levels in HT29 cells were lower 48 h after incubated with SDF-1 at the concentrations of 20 and 40 ng/mL （P 〈 0.05）. SDF-l-induced significant phosphorylation of PI3K/AKT and β-catenin. AMD3100 and LY294002 inhibited the phosphorylation of PI3K/AKT and β-catenin. CONCLUSION： SDF-1 down-regulates the E-cadherin/ β-catenin complex expression in HT29 cells by decreasing mRNA synthesis and increasing β-catenin phosphorylation.

关键词： CXCR4 Stromal cell-derived factor-i E-cad-herin β-catenin Phosphatidylinositol 3-kinase/AKT Co-lon cancer

β-catenin、Wnt1、Smad4、Hoxa9、Bmi-1与食管鳞癌预后的关系

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世界华人消化杂志 2010年第27期18卷 2874-2883页

作者：吕进曹秀峰纪律朱斌陶磊王冬冬南京医科大学附属南京第一医院肿瘤外科南京医科大学肿瘤中心江苏省南京市210006

目的:探讨β-catenin、Wnt1、Smad4、Hoxa9、Bmi-1基因与食管鳞癌(esophageal squamous cell carcinoma,ESCC)预后及临床病理的关系.方法:从南京医科大学附属第一医院2002-07/2003-12106例ESCC石蜡标本中随机选取70例.运用免疫组织化学及实时定量PCR检测70例ESCC患者肿瘤切除石蜡标本中待测基因的mRNA及蛋白表达水平,统计学分析其与ESCC预后及临床病理的关系.结果:ESCC中的β-catenin、Wnt1、Smad4、Hoxa9、Bmi-1mRNA表达水平明显高于癌旁组织(0.0821±0.0416vs0.0185±0.0201,P=0.0000;1.9934±1.9888vs0.8863±0.6658,P=0.0184;0.0298±0.0215vs0.0189±0.0187,P=0.0017;2.098±0.091vs1.016±0.078,P=0.0000;2.181±2.158vs0.931±0.894,P=0.0152).ESCC中的β-catenin、Wnt1、Smad4、Hoxa9、Bmi-1的蛋白水平明显高于癌旁组织(0.2835±0.0844vs0.2352±0.0670,P=0.0003;0.3830±0.0947vs0.2721±0.1474,P=0.0000;0.2637±0.0348vs0.2042±0.0180,P=0.0000;0.2058±0.0316vs0.1218±0.0518,P=0.0000;0.2736±0.0834vs0.2251±0.0571,P=0.0001).除Smad4外,待测基因mRNA及蛋白质表达水平均与病理分期和淋巴结转移相关(均P0.05).Smad4mRNA表达水平与病理分期和淋巴结转移相关(均P0.05),与患者预后无关(P>0.05).Logistic多因素分析显示预后有关的因素有β-catenin、Wnt1、Bmi-1表达阳性或Wnt1及β-catenin二者表达阳性者或Wnt1、β-catenin、Bmi-1三者表达阳性者(χ2=17.65,P=0.0072).结论:Wnt1/β-catenin、Bmi-1高表达者预后不佳,表达水平与ESCC临床病理分期、淋巴结转移呈逆相关;Smad4、Hoxa9mRNA表达水平虽与ESCC预后及临床病理分期、淋巴结转移相关,但可能不是独立的预后因素.

关键词：食管鳞癌 β-catenin Wnt-1 Bmi-1 Smad4 Hoxa9 预后临床病理

Reduced expression ofβ-catenin inhibitor Chibby in colon carcinoma cell lines

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World Journal of Gastroenterology 2006年第10期12卷 1529-1535页

作者： Marion M Schuierer Elisabeth Graf Ken-Ichi Takemaru Wolfgang Dietmaier Anja-Katrin Bosserhoff Institute of Pathology University of Regensburg Franz-Josef-Strauss-Alice 1193053 RegensburgGermany Department of Pharmacology BST 7-168 Stony BrookNY 11794-8651United States Institute of Pathology University of Regensburg Franz-Josef-Strauss-Allee 1193053 RegensburgGermany

AIM： To analyse the Chibby expression and its function in colon carcinoma cell lines and colorectal carcinoma （CRC）. METHODS： Chibby expression levels were investigated by quantitative RT-PCR in a panel of seven different colon carcinoma cell lines. By sequencing, we analysed mutational status of Chibby. To test whether Chibby exhibited effects on β-catenin signalling in colon carcinoma cells, we transfected SW480 cells with Chibby expression plasmid and, subsequently, analysed activity of β-catenin and tested for alterations in cellular phenotype. In addition, we examined Chibby mRNA levels in samples of colorectal carcinomas and adjacent normal tissues by using quantitative RT-PCR and hybridised gene chips with samples from CRC and normal tissues. RESULTS： Chibby mRNA expression was strongly downregulated in colon carcinoma cell lines in comparison to normal colon epithelial cells and no mutation in any of the examined colon carcinoma cell lines was found. Further, we could show that Chibby inhibited β-catenin activity in TOPflash assays when over-expressed in SW480 cells. Proliferation and invasion assays with Chibby transfected SW480 cells did not reveal profound differences compared to control cells. In contrast to these in vitro data, quantitative RT-PCR analyses of Chibby mRNA levels in CRC tumor samples did not show significant differences to specimens in adjacent non-cancerous tissue. Consistent with these findings, gene chips analysing tissue samples of tumors and corresponding normal tissue did not show altered Chibby expressionCONCLUSION： Altered Chibby expression might be observed in vitro in different colon carcinoma cell lines. However, this finding could not be confirmed in vitro in CRC tumors, indicating that Chibby is not likely to promote CRC tumor development or progression. As Chibby is an important inhibitor of β-catenin signalling, our data implicate that the usability of colon carcinoma cell lines for in vitro studies analysing the Wnt/β-ca

关键词： Chibby Wnt β-catenin Colorectal carcinoma Colon carcinoma cell lines

MUC5AC/β-catenin expression and KRAS gene alteration in laterally spreading colorectal tumors

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World Journal of Gastroenterology 2012年第39期18卷 5551-5559页

作者： Kosaburo Nakae Hiroyuki Mitomi Tsuyoshi Saito Michiko Takahashi Takashi Morimoto Yasuhiro Hidaka Naoto Sakamoto Takashi Yao Sumio Watanabe Department of Gas-troenterology Juntendo University School of Medicine2-1-1 HongoBunkyo-kuTokyo 113-8421Japan Department of Human Pathology Juntendo University School of Medicine1-1-19 HongoBunkyo-kuTokyo 113-0033Japan

AIM： To clarify differences in mucin phenotype, prolif- erative activity and oncogenetic alteration among sub- types of colorectal laterally spreading tumor （LST）. METHODS： LSTs, defined as superficial elevated lesions greater than 10 mm in diameter with a low vertical axis, were macroscopically classified into two subtypes：（1） a granular type （Gr-LST） composed of superficially spread- ing aggregates of nodules forming a fiat-based lesion with a granulonodular and uneven surface; and （2） a non-granular type （NGr-LST） with a flat smooth surface and an absence of granulonodular formation. A total of 69 LSTs, comprising 36 Gr-LSTs and 33 NGr-LSTs, were immunohistochemically stained with MUC2, MUC5AC, MUC6, CD10 （markers of gastrointestinal cell lineage）, p53, 13-catenin and Ki-67 antibodies, and examined for alteration in exon 1 of v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog （KRAS） and exon 15 of v-raf murine sarcoma viral oncogene homologue B1 （BRAF） by poly- merase chain reaction followed by direct sequencing. RESULTS： Histologically, 15 Gr-LST samples were ad- enomas with low-grade dysplasia （LGD）, 12 were high- grade dysplasia （HGD） and 9 were adenocarcinomas invading the submucosa （INV）, while 12 NGr-LSTs demonstrated LGD, 14 HGD and 7 INV. In the proximal colon, MUC5AC expression was significantly higher in the Gr-type than the NGr-type. MUC6 was expressed only in NGr-LST. MUC2 or CD10 did not differ. P53 ex- pression demonstrated a significant stepwise increment in progression through LGD-HGD-INV with both types of LST. Nuclear β-catenin expression was significantly higher in the NGr-type. Ki-67 expression was signifi- cantly higher in the Gr-type in the lower one third zone of the tumor. In proximal, but not distal colon tumors, the incidence of KRAS provided mutation was signifi- cantly higher in the Gr-type harboring a specific muta- tional pattern （G12V）. BRAF mutations （V600E） were detected only in two Gr-LSTs. CONCLUSION： The two subtypes of LST,

关键词： Laterally spreading tumor Mucin core pro-tein Colon β-catenin Immunohistochemistry v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog Directsequencing Adenoma-carcinoma sequence

β-catenin基因在Peutz-Jeghers综合征组织中的突变及表达

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世界华人消化杂志 2011年第16期19卷 1739-1744页

作者：占春玲吴保平张亚历长沙市第一医院感染二科湖南省长沙市410005 南方医科大学南方医院消化内科广东省广州市510515

目的:研究Peutz-Jeghers综合征(PJS)患者体内β-catenin基因突变及其蛋白表达,探讨β-catenin在PJS息肉发生及恶变中的作用.方法:收集PJS息肉、癌旁正常黏膜各12例,结肠腺瘤、结肠癌组织各14例,及相应PJS血液标本,检测β-catenin基因外... 详细信息

目的:研究Peutz-Jeghers综合征(PJS)患者体内β-catenin基因突变及其蛋白表达,探讨β-catenin在PJS息肉发生及恶变中的作用.方法:收集PJS息肉、癌旁正常黏膜各12例,结肠腺瘤、结肠癌组织各14例,及相应PJS血液标本,检测β-catenin基因外显子3突变及mRNA表达水平.选取PJS息肉、结肠腺瘤、结肠癌、结肠正常黏膜组织石蜡标本各20例,通过免疫组织化学SP法检测β-catenin蛋白表达情况.结果:PJS息肉组织RT-PCRβ-cateninIA/GAPDHIA与结肠腺瘤、结肠癌、癌旁正常组织相比,差异无显著性.7例(70%)PJS息肉组织、2例(66.7%)PJS肠道正常黏膜、3例(50%)PJS血液白细胞出现编码区431位碱基缺失突变;8例(80%)PJS息肉组织、1(33.3%)例PJS肠道正常黏膜、4例(66.7%)PJS血液白细胞、2例(100%)PJS家族正常成员出现非编码区184位插入突变;1(50%)例PJS家族正常成员、2例(33.3%)PJS血液白细胞编码区421位缺失突变;2(20%)例PJS息肉组织非编码区189位插入突变.PJS、腺瘤、腺癌及正常组织中,β-catenin膜表达缺失率分别为30.0%、45.0%、75.0%和5.0%;细胞质阳性表达率和表达强度呈增加趋势;胞核阳性表达率分别为60.0%、30.0%、80.0%、5.0%;总的异位表达率分别为60.0%、50.0%、90.0%、5.0%,差异均具有显著性(Pβ-catenin蛋白胞质内异常聚集并非mRNA表达量增高所致.PJS息肉组织中β-catenin基因突变较为常见,PJS不仅存在体系突变,也存在胚系突变,431、421、184、189这4个碱基位点可能是PJS有别于其他肿瘤的新的突变位点.β-catenin蛋白表达异常可能是PJS息肉形成及恶变的特征之一.

关键词： Peutz-Jeghers综合征 β-catenin 癌易感综合征基因突变蛋白表达

Altered Wnt Signaling Pathway in Cognitive Impairment Caused by Chronic Intermittent Hypoxia： Focus on Glycogen Synthase Kinase-3β and β-catenin

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Chinese Medical Journal 2016年第7期129卷 838-845页

作者： Yue-Ying Pan Yan Deng Sheng Xie Zhi-Hua Wang YuWang Jie Ren Hui-Guo Liu Department of Respiratory and Critical Care Medicine Tongji Hospital Tongli Medical College Huazhong University of Science and Technology Key Laboratory of Respiratory Disease of the Ministry of Health Wuhan Hubei 430030 China

Background： Cognitive impairment is a severe complication caused by obstructive sleep apnea （OSA）. The mechanisms of causation are still unclear. The Wntβ-catenin signaling pathway is involved in cognition, and abnormalities in it are implicated in neurological disorders. Here, we explored the Wntβ-catenin signaling pathway abnormalities caused by chronic intermittent hypoxia （CIH）, the most characteristic pathophysiological component of OSA. Methods： We divided 32 4-week-old male C57/BL mice into four groups of eight each： a CIH ＋ normal saline （NS） group, CIH ＋ LiCI group, sham CIH ＋ NS group, and a sham CIH ＋ LiCI group. The spatial learning performance of each group was assessed by using the Morris water maze （MWM）. Protein expressions of glycogen synthase kinase-β （GSK-β） and β-catenin in the hippocampus were examined using the Western blotting test. EdU labeling and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling staining methods were used, respectively, to determine the proliferation and apoptosis of neurons in the hippocampal dentate gyrus region. Results： Mice exposed to CIH showed impaired spatial learning performance in the MWM, including increased mean escape latencies to reach the target platform, decreased mean times passing through the target platform and mean duration in the target quadrant. The GSK-313 activity increased, and expression of β-catenin decreased significantly in the hippocampus of the CIH-exposed mice. Besides, CIH significantly increased hippocampal neuronal apoptosis, with an elevated apoptosis index. Meanwhile, LiCI decreased the activity of GSK-β and increased the expression of β-catenin and partially reversed the spatial memory deficits in MWM and the apoptosis caused by CIH. Conclusions： Wntβ-catenin signaling pathway abnormalities possibly play an important role in the development of cognitive deficits among mice exposed to CIH and that LiCI might attenuate CIH-induced cognitive impairment via Wntβ-c

关键词： β-catenin Chronic Intermittent Hypoxia Cognition Glycogen Synthase kinase-3β Hippocampus Obstructive SleepApnea

Caveolin-1,E-cadherin and β-catenin in Gastric Carcinoma,Precancerous Tissues and Chronic Non-atrophic Gastritis

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Chinese Journal of Cancer Research 2012年第1期24卷 23-28页

作者： Guo-yang Sun Jun-xia Wu Jian-sheng Wu Yu-ting Pan Rong Jin Department of Gastroenterology the First Affiliated HospitalWenzhou Medical CollegeWenzhou 325000China Department of Gastroenterology Shaoxing Municipal People's HospitalShooxing 312000China Department of Epidemiology the First Affiliated HospitalWenzhou Medical CollegeWenzhou 325000China

Objective： To investigate the expressions of caveolin-1, E-cadherin and β-catenin in gastric carcinoma, precancerous gastric and chronic non-atrophic gastritis tissues, and evaluate the correlation of these expressions with the development of gastric cancer. Methods： The expressions of caveolin-1, E-cadherin and β-catenin were detected by biotin-streptavidinperoxidase （SP） immunohistochemistry on 58 gastric cancer tissues, 40 precancerous gastric tissues and 42 chronic non-atrophic gastritis tissues. The correlation between the expressions of caveolin-1, E-cadherin and β-catenin, and the clinicopathologic parameters of gastric cancer was analyzed retrospectively. Results： The positive rates of caveolin-1 and E-cadherin expressions in gastric carcinoma were significantly lower than precancerous gastric and chronic non-atrophic gastritis tissues （P〈0.01）. An abnormal rate of β-catenin expression in gastric carcinoma was higher than precancerous gastric and chronic non-atrophic gastritis tissues （P〈0.01）. Moreover, low expressions of caveolin-1, E-cadherin and β-catenin correlated with tumor size, depth of invasion, lymph node metastasis and TNM stage （P〈0.05）. The positive rates of caveolin-1 and E-cadherin expressions decreased （P〈0.01）, while an abnormal rate of β-catenin expression increased inversely, with the degree of atypical hyperplasia （P〈0.01）. Caveolin-1 expression correlated positively with E-cadherin （r=0.41, P〈0.05）. Caveolin-1 （r=-0.36, P〈0.05） and E-cadherin （r=-0.45, P〈0.05） expressions negatively correlated with abnormal β-catenin expression. Conclusion： These results suggested that dysregulated expressions of caveolin-1, E-cadherin and β-catenin correlated with the development of gastric cancer and its biological behavior.

关键词： Gastric carcinoma Caveolin-1 E-cadherin β-catenin Immunohistochemistry

β-catenin蛋白在散发性结直肠癌中的异常表达和基因突变

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世界华人消化杂志 2009年第23期17卷 2385-2390页

作者：覃业军周晓燕施达仁山东大学附属省立医院病理科山东省济南市250021 复旦大学附属肿瘤医院病理科上海市200032

目的:观察β-catenin蛋白在散发性结直肠癌(SCRC)中胞质胞核异常表达特征和基因突变发生率,并探讨其与临床病理的相关性.方法:用免疫组织化学EnVision二步法检测90例SCRC与22例散发性结直肠腺瘤标本的β-catenin蛋白在胞质胞核中的异常... 详细信息

目的:观察β-catenin蛋白在散发性结直肠癌(SCRC)中胞质胞核异常表达特征和基因突变发生率,并探讨其与临床病理的相关性.方法:用免疫组织化学EnVision二步法检测90例SCRC与22例散发性结直肠腺瘤标本的β-catenin蛋白在胞质胞核中的异常表达.以PCR扩增和直接测序检测上述标本中β-catenin基因突变.结果:正常结直肠黏膜中β-catenin蛋白表达定位于细胞膜上,未见胞质胞核中的异常表达.SCRC中β-catenin蛋白在胞质与胞核的异常表达率显著高于散发性腺瘤中的异常表达率(88.9%vs59.1%,41.1%vs18.2%,Pβ-catenin异常胞质表达率与肿瘤生长方式,分化程度相关(Pβ-catenin基因突变;22例散发性结直肠腺瘤中未检测出突变.结论:β-catenin蛋白胞质胞核异常表达可能在SCRC的发生发展中起重要作用.在SCRC中β-catenin基因突变可能不是β-catenin蛋白异常表达的主要原因.

关键词：散发性结直肠癌 β-catenin 免疫组织化学基因突变

1α,25-dihydroxyvitamin D 3 inhibits transforming growth factorβ1-induced epithelial-mesenchymal transition viaβ-catenin pathway

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Chinese Medical Journal 2020年第11期133卷 1298-1303页

作者： Xin-Rong Xiong Xin-Li Tian Ru-Jie Huo Yan-Ting Dong Dai Liu Jing-Cui Bai Yun-Feng Qi Xin-Rui Tian Shanxi medical university TaiyuanShanxi 030001China Cardiopulmonary Center The Seventh Medical Center of People's Liberation Army General HospitalBeijing 100730China Department of Respiratory and Critical Care Medicine The Second Hospital of Shanxi Medical UniversityTaiyuanShanxi 030001China Department of Respiratory Medicine Jinhua Hospital of Traditional Chinese MedicineJinhuaZhejiang 321017China

Background::The transforming growth factorβ1(TGF-β1)-induced epithelial-mesenchymal transition(EMT)has been proven associated with the pathogenesis of asthmatic airway remodeling,in which the Wnt/β-catenin pathway plays an important role,notably with regard to TGF-β*** studies have shown that 1α,25-dihydroxyvitamin D 3(1α,25(OH)2D 3)inhibits TGF-β1-induced EMT,although the underlying mechanism have not yet been fully ***::Alveolar epithelial cells were exposed to 1α,25(OH)2D 3,ICG-001,or a combination of both,followed by stimulation with TGF-β*** protein expression of E-cadherin,α-smooth muscle actin,fibronectin,andβ-catenin was analyzed by western blotting and immunofluorescence *** mRNA transcript of Snail was analyzed using RT-qPCR,and matrix metalloproteinase 9(MMP-9)activity was analyzed by gelatin *** activity of the Wnt/β-catenin signaling pathway was analyzed using the Top/Fop flash ***::Both 1α,25(OH)2D 3 and ICG-001 blocked TGF-β1-induced EMT in alveolar epithelial *** addition,the Top/Fop Flash reporters showed that 1α,25(OH)2D 3 suppressed the activity of the Wnt/β-catenin pathway and reduced the expression of target genes,including MMP-9 and Snail,in synergy with ***::1α,25(OH)2D 3 synergizes with ICG-001 and inhibits TGF-β1-induced EMT in alveolar epithelial cells by negatively regulating the Wnt/β-catenin signaling pathway.

关键词： 1α,25-Dihydroxyvitamin D3 Vitamin D β-catenin Epithelial-mesenchymal transition Airway remodeling Asthma