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Enhanced a novel β-agarase production in recombinant escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy

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Acta Oceanologica Sinica 2018年第2期37卷 110-118页

作者： CHAN Zhuhua CHEN Xinglin HOU Yanping GAO Boliang ZHAO Chungui YANG Suping ZENG Runying Department of Bioengineering and Biotechnology Huaqiao University State Key Laboratory Breeding Base of Marine Genetic Resource Third Institute of Oceanography State Oceanic Administration School of Life Sciences Xiamen University

agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates（μ） was applied in the pre-induction phase. The results showed that the low μ（μ=0.2） was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside（IPTG）, the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/（L·h）, the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to ***, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose（NA4） and neoagarohexaose（NA6）. The overall research may be useful for the industrial production and application of β-agarase.

关键词： β-agarase escherichia coli process optimization glycerol feeding strategy neoagarooligosaccharide

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