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Circular dichroism spectra of α-lactose molecular measured by terahertz time-domain spectroscopy

Chinese Physics B 2022年第10期31卷 406-409页

作者：王春王博魏高帅陈佳宁汪力 Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China

Researchers have long studied circular dichroism(CD) for its enormous prospects in life sciences. Many biomolecules have vibration modes in the terahertz region, and terahertz CD spectra are robust to detect biomolecular structures. However,few studies explore the terahertz CD spectra on even natural materials due to technical challenges in both fields. Here, we report a setup of home-built terahertz time-domain spectroscopy to measure the polarization states of terahertz *** carefully measuring the transmission Jones matrix, we obtain terahertz CD spectra of α-lactose tablets and D-glucose tablets. Our results show that the terahertz CD spectra are sensitive to vibrational motions in biochemical compounds,which will find wide applications in biosensing and biomedical diagnostics.

关键词： circular dichroism terahertz α-lactose

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Terahertz Time-Domain Spectroscopy to Identify and Evaluate Anomer in lactose

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American Journal of Analytical Chemistry 2013年第12期4卷 756-762页

作者： Satoshi Yamauchi Sakura Hatakeyam Yoh Imai Masayoshi Tonouchi Department of Biomolecular Functional Engineering Ibaraki University Hitachi Japan Department of Electric and Electronic Engineering Ibaraki University Hitachi Japan Institute of Laser Engineering Osaka University Osaka Japan

lactose powder consisting of α-D-lactose monohydrate and anhydrous β-D-lactose was nondestructively and quantitatively evaluated by transmission-type Terahertz time-domain spectroscopy (THz-TDS). An absorption with peak at 39.7 cm-1 (1.19 THz) was assigned to be derived from anhydrous β-D-lactose, in addition to the absorptions due to α-D-lactose monohydrate with peak at 17.1 cm-1 (0.53 THz) and 45.6 cm-1 (1.37 THz). After deconvolution of the spectra using Lorentzian, integrated intensities of the absorptions with peak at 39.7 cm-1 and 45.6 cm-1 were uniquely dependent on the weight composition ratio of the α-and β-lactose powder. As a result, the net molar-ratio of the α-and β-lactose in lactose powder could be precisely evaluated by the integrated intensity ratio. Further, anomer content in lactose powder extracted from lactose solution was evaluated and the refined and unrefined features were shown by the evaluation method.

关键词： THz-TDS lactose-Powder Anomer α-lactose β-lactose

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