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ONCOGENIC　TRANSFORMATION　OF　SYRIAN　HAMSTER　EMBRYO　CELLS　BY　5．3－MeV　α　particles　AND　A　TUMOR　PROMOTER　PHORBOL　ESTER　

Chinese Medical Sciences Journal 1996年第4期11卷 195-199页

作者：寿江龚诒芬吴德昌 Institute of Radiation Medicine Beijing.

The primary Syrian hamster embryo(SHE) cells were used to study the oncogenic transformation by ￣(238)pu α particles or X-rays alone or in combination with a chemical promoter phorbol *** curves of SHE cells following exposure to α-particles or X-rays were fitted to single-or multi-target models,respectively. Model parameters were: Do = 0. 55 Gy. n = 1 for α particles 4 Do = 1.44 Gy. Dq = 3.0 Gy. n=7.7 for *** of α particles or X-rays induced cell transformation was dose-dependant.α particles were more efficient in inducing cell transformation than that of X-rays. The enhancement of SHE cell transformation by phorbol 12-myristate 13-acetate(PMA) following exposure to α particles of 0. 25-1. 00 Gy was observed.

关键词： oncogenic transformation syrian hamster embryo cells α particles

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PARTIAL　DELTETION　OF　p53　GENE　IN　α　PARTICLE－INDUCED　TRANSFORMANT　OF　SYRIAN　HAMSTER　EMBRYO　CELLS

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Chinese Medical Sciences Journal 1996年第3期11卷 162-165页

作者：寿江章扬培吴德昌

The mutation of p53 gene was detected in Syrian hamster embryo(SHE) cells neoplastically initiated with a *** level of the p53 mRNA in transformant was obviously higher than that in non-irradiated counterpart,as measured by Northern blot analysis of total RNA.A pair of primers were designed based on p53 cDNA sequence to produce the whole length of coding sequence about 1.2 kilobase(Kb) by reverse transcription of mRNA followed by the polymerase chain reaction(RTPCR),but the length of fragment amplified from transformant mRNA was about 0.3Kb,remarkably shorter than that from normal SHE *** analysis of p53 protein showed that no heavy staining was found on slice of tumor derived from transformant inoculated in nude mice with hamster specific p53 monocloned antibody *** results implied that p53 gene had been mutated by deletion,which might lead to loss of p53 protein expression but the increased expression of p53 remained in a particle-induced SHE transformant.

关键词： α particles p53 gene partial deletion

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