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Cluster of differentiation antigens:essential roles in the identification of teleost fish t lymphocytes

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Marine Life Science & technology 2022年第3期4卷 303-316页

作者： Hong‑fei tian Jing Xing Xiao‑qian tang Heng Chi Xiu‑zhen Sheng Wen‑bin Zhan Laboratory of Pathology and Immunology of Aquatic Animals KLMMEFisheries CollegeOcean University of ChinaQingdao 266003China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

Cluster of differentiation(CD)antigens are cell surface molecules expressed on leukocytes and other cells associated with the immune *** that react with CD antigens are known to be one of the most essential tools for identifying leukocyte subpopulations.t lymphocytes,as an important population of leukocytes,play essential roles in the adaptive immune *** of the CD antigens expressed on t lymphocytes are used as surface markers for t lymphocyte classification,including CD3,CD4 and CD8 *** this review,we summarize the recent advances in the identification of CD molecules on t lymphocytes in teleosts,with emphasis on the functions of CD markers in the classification of t lymphocyte *** notice that genes encoding CD3,co-receptors CD4 and CD8 have been cloned in several fish species and antibodies have been developed to study protein expression in morphological and functional contexts.t lymphocytes can be divided into CD4^(+)and CD8^(+)cells discriminated by the expression of CD4 and CD8 molecules in teleost,which are functionally similar to mammalian helper t cells(th)and cytotoxic t cells(tc),*** studies are still needed on the particular characteristics of teleost t cell repertoires and adaptive responses,and results will facilitate the health management and development of vaccines for fish.

关键词： t lymphocytes Surface marker Classification Monoclonal antibody Fish

Effect of Chinese Herbal Medicinal Ingredients on IL-2 mRNA Levels of t lymphocytes in Mice Measured Using Semiquantification Rt-PCR

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Agricultural Sciences in China 2006年第11期5卷 873-878页

作者： CHU Yue-feng YAN Xin-min LI Xiang-rui HU Yuan-liang College of Veterinary Medicine Nanjing Agricultural University Nanjing 210095 P.R.China Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 P.R.China

In this study, the IL-2 mRNA levels of t lymphocytes in normal mice stimulated by nine Chinese herbal medicinal ingredients （CHMIs） were measured using semiquantification reverse transcription polymerase chain reaction. the results showed that astragalus polysaccharide （APS）, epimedium polysaccharide （EPS）, Chinese angelica polysaccharide （CAPS）, propolis flavone （PF）, and astrogalosides （AS） promoted IL-2 mRNA levels in t lymphocytes in vitro and in vivo to differing degrees, and the level of IL-2 mRNA induced by propolis polysaccharide （PPS） in vitro was higher than that induced by the control, which differed from that of PPS in vivo.

关键词： Chinese herbal medicinal ingredients semiquantification Rt-PCR IL-2 mRNA t lymphocytes mice

High mobility group box 1 protein(HMGB1)as an immune-modulating factor for polarization of human t lymphocytes

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Journal of Geriatric Cardiology 2008年第2期5卷 117-122页

作者： Lifeng Huang Yongming Yao Haidong Meng Xiaodong Zhao Ning Dong Yan Yu Department of Microbiology and Immunology Burns Institutethe First Hospital Affiliated to the Chinese PLA General HospitalBeijing 100037China

Objective this study was performed to investigate the effect of high mobility group box-1 protein(HMGB1)on immune function of human t lymphocytes in vitro and explore its potential role in cell-mediated immune *** Fresh blood was obtained from healthy adult volunteers and peripheral blood mononuclear cells(PBMCs)were isolated,then rhHMGB1 was added to ***-color flow cytometric(FCM)analysis was used for the measurement of intracellular cytokine including interleukin IL-4 and interferon IFN-?ELISA kits were employed for the determination of IL-2 and sIL-2R protein levels in cell culture ***(1)Different stimulating time and dosage of rhHMGB1 did not alter the number of IFN-αpositive cells(th1).rhHMGB1 stimulation provoked a dose-dependent and time-dependent increase in th2 subset and decrease in ratio of th1 to th2.(2)Compared with the untreated cells,when the cells were coincubated with rhHMGB1(10-100ng/ml)for 12 hrs,protein release of IL-2 and sIL-2R were significantly *** 48 hrs,in contrast,protein production was relatively lower in cells after exposure to 100-1000 ng/ml *** these findings demonstrated that HMGB1 has a dual influence on immune functions of human t lymphocytes.

关键词： High mobility group box-1 protein Immunity t lymphocytes th1/th2

tHE ADAPtABLE CHANGE OF tHE FUNCtION OF t lymphocytes FOR PHYSICAL EXERCISE WItH OXYGEN

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Academic Journal of Xi'an Jiaotong University 2001年第1期13卷 68-69,76页

作者：王军阳袁育康范桂香任会勋史霖 Department of Immunology Med. Coll. of Xi'an Jiaotong Univ. Xi'an 710061 China

Objective to find out the possible regularity and mechanism of the adaptable change of human be- ing t lymphocytes for physical exercise with oxygen and bring the original data for the Movement of All People Im- proving their Health. Methods We selected 16 untrained female students in university and let them had the same amount of exercise for 8 weeks. After that, we collected the cycle blood at the time point or before exercise, the end of exercise and 1 hour after exercise at the end or the 0,first, 2 nd, 4 nd, 6 nd and 8 nd week respectively, so as to de- termine its stimuli index (SI) by Mtt method. Results In the different time sect, such as the early stage or exer- cise, quiet condition,as soon as the end of exercise and 1 hour after exercise, we found that the SI were obviousIy low- er than that or normal (Pthe time sect of the end or exercise. Continuing to 4 weeks,the func- tion of t lymphocytes restored gradualy and it lasted to the 8th week, the SI in quiet condition and 1 hour after exer- cise had restored to normal (P>0.05),but in the end or exercise, it still was low,however. the extent of the cases se- lected was in a condition or acute excitability. Conclusion As the bodies adapting to the exercise, the function of t lymphocytes restored slowly and the rate increased faster and faster.

关键词： Physical exercise with oxygen t lymphocytes Mtt

Ambiguous nucleus regulates the proliferation and percentage of t lymphocytes in peripheral blood

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Neural Regeneration Research 2011年第35期6卷 2761-2767页

作者： Wei Wang Wei Chen Yingwu Mei Bin Guo Zhanqing Yang Shoupeng Fu Zhanpeng Yue Juxiong Liu College of Animal Science and Veterinary Medicine Jilin University Changchun 130062 Jilin Province China

the aim of this study was to examine the immunomodulatory role of the unilateral ambiguous nucleus （Amb）. We performed electrical stimulation of the unilateral Amb, electrical stimulation of the left parietal cortex and the lateral hypothalamus following unilateral Arab lesion, as well as microinjection of acetylcholine chloride and hemicholine-3 into the unilateral Amb, and electrical stimulation of the unilateral Amb after injection of atropine, mecamylamine, propranolol, and phentolamine. Results showed that the number and proliferation of peripheral blood t lymphocytes were increased after electrical stimulation of the unilateral Arab. the cholinergic neurons in the Amb released choline substances to alter cellular immunity, thus confirming that the Amb mediates the neuro-immunomodulatory process.

关键词： ambiguous nucleus electrical stimulation t lymphocytes proliferation neuroimmune regulation parasympathetic nervous system

Phenotypic and Functional Analysis of Porcine t lymphocytes

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tsinghua Science and technology 2001年第3期6卷 273-276页

作者：李华陈应华 Laboratory of Immunology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Porcine and other higher mammals express clusters of differentiation (CD) antigens on the surface of t lymphocytes, such as CD2, CD3, CD4, CD8, etc. However, in porcine, a high percentage of the CD4 +CD8 + t lymphocyte subpopulation exist in the peripheral blood and the ratio of the CD4 + and CD8 + t lymphocyte subpopulations is reversed. these differences bring new challenges to better understanding of the phenotype and function of porcine t lymphocytes in antigen recognition and immune response. 

关键词： swine t lymphocytes phenotype function

Protective Effects of Lentinan against t lymphocytes Injury in Mice under Chronic Radiation Stress

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Chinese Herbal Medicines 2013年第1期5卷 62-66页

作者： WANG Yong LI Ming-chun FU Qing-jie Medical College of Qingdao University

Objective to study the effects of lentinan (LtN) on mice exposed to chronic radiation. Methods Animals were divided into three groups (n = 10), they were animals exposed to radiation (Rad), normal control animals (Ctr), and irradiated animals treated with LtN (Rad + LtN). Animal model of chronic radiation stress injury was induced by irradiating mice with 60 Co γ-ray for 6 weeks from Monday to Friday consecutively. Before radiation, the mice in Rad + LtN group were ip injected with 0.5 mL LtN (0.01 mg/mL), whereas mice in other groups were injected with 0.9% physiological saline. the effects of LtN treatment on irradiated mice were examined by histological analysis on the spleen. the cell numbers and viability of t lymphocytes, which were isolated from the spleen, were determined by trypan blue staining. Nitric oxide (NO) production and interleukin-2 (IL-2) secretion in t lymphocytes were also measured. Results Chronic radiation significantly reduced the body weights and the spleen and thymus indexes, associated with reduced t lymphocytes viability and functions, and elevated NO production. treatment with LtN significantly normalized the elevated NO production, and attenuated the negative outcomes resulting from radiation mentioned above. Conclusion the results suggest that radioprotective effect of LtN may be contributed by improved t lymphocytes viability and functions via regulating the NO and IL-2 production in t lymphocytes.

关键词： chronic radiation stress interleukin-2 lentinan nitric oxide t lymphocytes

Interleukin-12 induces gene expression in interleukin-2 stimulated human t lymphocytes

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Interleukin-12 induces gene expression in interleukin-2 stim...

Correlative factors of poor prognosis and abnormal cellular immune function in patients with Alzheimer’s disease

作者： Jacques theze Division of Cellular Immunol and Immunogen Institute PasteurParisFrance

IL-12 is a critical immunoregulatory cytokine that promotes cell-mediated immune responses by inducing the differentiation of thl *** better clarify the molecular basis of IL-12 action, we compared the gene expression in human t lymphocytes activated by IL-2 and *** from t lymphocytes activated by either IL-2 alone or IL-2 plus IL-12 were transcribed into cDNAs.A differential mRNA display was conducted. As a result ,differential display of five cDNA

关键词： IL-12 IL-2 t lymphocytes C-type lectin glucose transporter-like protein

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World Journal of Clinical Cases 2024年第6期12卷 1063-1075页

作者： Hua Bai Hong-Mei Zeng Qi-Fang Zhang Yue-Zhi Hu Fei-Fei Deng Department of Neurology The Third Affiliated Hospital of Guizhou Medical University in ChinaDuyun 558099Guizhou ProvinceChina Department of Neurology Guizhou Medical UniversityDuyun 558099Guizhou ProvinceChina Key Laboratory of Medical Molecular Biology Guizhou Medical UniversityGuiyang 550004Guizhou ProvinceChina

BACKGROUND Alzheimer’s disease(AD)is a serious disease causing human dementia and social *** quality of life and prognosis of AD patients have attracted much *** role of chronic immune inflammation in the pathogenesis of AD is becoming more and more *** to study the relationship among cognitive dysfunction,abnormal cellular immune function,neuroimaging results and poor prognostic factors in *** A retrospective analysis of 62 hospitalized patients clinical diagnosed with AD who were admitted to our hospital from November 2015 to November *** cognitive dysfunction performance characteristics,laboratory test data and neuroimaging data from medical records within 24 h of admission,including Mini Mental State Examination Scale score,drawing clock test,blood t lymphocyte subsets,and neutrophils and lymphocyte ratio(NLR),disturbance of consciousness,extrapyramidal symptoms,electroencephalogram(EEG)and head nucleus magnetic spectroscopy(MRS)and other *** logistic regression analysis was used to determine independent prog-nostic *** modified Rankin scale(mRS)was used to determine whether the prognosis was *** correlation between drug treatment and prognostic mRS score was tested by the rank sum *** Univariate analysis showed that abnormal cellular immune function,extrapyramidal symptoms,obvious disturbance of consciousness,abnormal EEG,increased NLR,abnormal MRS,and complicated pneumonia were related to the poor prognosis of AD *** logistic regression analysis showed that the decrease in the proportion of t lym-phocytes in the blood after abnormal cellular immune function(odd ratio:2.078,95%confidence interval:1.156-3.986,Pt risk factor for predicting the poor prognosis of *** number of days of donepezil treatment to improve cognitive function was negatively correlated with mRS score(r=0.578,Pthe decrease in the proportion of t

关键词： Alzheimer’s disease Cellular immunity Prognosis t lymphocytes Magnetic resonance spectroscopy