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高等学校化学学报 2003年第9期24卷 1585-1587页

作者：程伟黄卫华庞代文王宗礼程介克崔大付屈三甫郑从义武汉大学化学与分子科学学院分析科学中心武汉430072 中国科学院电子研究所传感技术国家重点实验室北京100080 武汉大学生命科学学院武汉430072

微流控芯片技术用于细胞生化分析已引起了广泛关注.

关键词：微流控芯片单细胞操纵实时监测单细胞活体量子释放碳纤维微电极生物化学分析

纳米电极时空分辨监测单个PC12细胞多巴胺量子释放

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高等学校化学学报 2003年第3期24卷 425-427页

作者：黄卫华张丽瑶程伟庞代文王宗礼程介克武汉大学化学与分子科学学院分析科学中心武汉430072

Carbon fiber nanoelectrodes(200-300 nm) were firstly used to amperometricaly monitor the dopamine release from single PC12 cells with temporal resolution and especially more higher spatial resolution than those obtained by using microelectrodes. When the nanoelectrode was in a distance 1 μm above the PC12 cell, only one peak signal corresponding to a single vesicle exocytotic event was detected caused from the stimulation with 1 mmol/L nicotine. The spatial difference of exocytosis was also detected by placing the electrode onto the different locations of the cell body, the results have demostrated that the spatial distribution of dopamine in cells is not uniform and the time for stimulating secretion is very different. Nanoelectrodes electrochemical method can provide a powerful tool for observing the temporal and spatial characteristics of the secretion from single cells directly.

关键词：碳纤维纳米电极时空分辨监测单细胞 PC12细胞多巴胺量子释放神经细胞囊泡

与量子释放缺陷相关的新型先天性肌无力综合征

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世界核心医学期刊文摘(神经病学分册) 2006年第10期 56-57页

作者： Milone M. Fukuda T. A.G. Engel 樊慧婷 Mayo Clinic 200 First Street S.W. Rochester MN 55905 United States Dr.

Background:Most congenital myasthenic syndromes are caused by defects in postsynaptic or synaptic basal lamina- associated proteins; congenital myasthenic syndromes (CMSs) associated with presynaptic defects are uncommon. Here, the authors describe clinical, electrophysiologic, and morphologic features of two novel and highly disabling CM- Ss, one determined by presynaptic and the other determined by combined presynaptic and postsynaptic defects. Methods: Microelectrode, single channel patch clamp, immunocytochemical, 125Iα - bungarotoxin binding, and quantitative electron microscopy studies of endplates were performed. Candidate genes were directly sequenced. Results: Patient 1, a 7- year- old boy, had severe myasthenic symptoms since infancy. Patient 2, a 48- year- old man, had delayed motor milestones and became progressively weaker after age 2 years. Both used wheelchairs and had a 30- 50% EMG decrement on 2- Hz stimulation. Evoked quantal release was reduced to approximately 25% of normal in both. In Patient 2, the synaptic response to acetylcholine was further compromised by degeneration of the junctional folds with concomitant loss of the acetylcholine receptor (AChR). A search for mutations in components of the synaptic vesicle release complex and in other candidate proteins failed to identify the molecular basis of the two syndromes. Conclusions: Combined clinical, morphologic, and in vitro electrophysiologic findings define two novel congenital myasthenic syndromes. The molecular basis of these syndromes awaits discovery.

关键词：突触后膜肌无力量子释放

在大白鼠不均匀牵张膈肌标本上量子释放和二项统计参数的计算

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中国应用生理学杂志 1990年第2期 127-127页

作者：纪占欣刘传绩军事医学科学院毒物药物研究所

已在甘油处理的肌肉和钳压肌肉标本上证明了突触前神经末梢递质释放的统计学性质为二项分布,但尚未在我们建立的接近正常生理状态的不均匀牵张膈肌标本(isolated nonuniform stretched muscle preparation,INSMP)上得到证实。递质释放... 详细信息

已在甘油处理的肌肉和钳压肌肉标本上证明了突触前神经末梢递质释放的统计学性质为二项分布,但尚未在我们建立的接近正常生理状态的不均匀牵张膈肌标本(isolated nonuniform stretched muscle preparation,INSMP)上得到证实。递质释放的统计学性质是计算递质释放参数的依据,是有关INSMP的基本数据。本实验观察在INSMP上递质释放的统计学特性,验证量子释放参数计算公式在INSMP上的应用。在INSMP上,同时记录同一终板的微终板电位(MEPP)和终板电位(EPP),其膜电位(MP)保

关键词：二项分布量子释放突触前泊松分布终板电位统计参数牵张标本

咖啡因引起平滑肌钙的量子释放依赖于肌浆内质网Ca2＋ATP酶的活性

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医学信息 1996年第8期 32-32页

作者：胡玉珍

咖啡因引起平滑肌钙的量子释放依赖于肌浆内质网ＣａＡＴＰ酶的活性［英］Ｊ．Ｍ．Ｓｔｅｅｎｂｅｒｇｅｎ，ｅｔａｌ．ＪＢｉｏｌｃｈｅｍ．１９９６；２７１(４)：１８２１－１８２４细胞内贮存钙的释放在一定程度上反应了１，４，５... 详细信息

咖啡因引起平滑肌钙的量子释放依赖于肌浆内质网ＣａＡＴＰ酶的活性［英］Ｊ．Ｍ．Ｓｔｅｅｎｂｅｒｇｅｎ，ｅｔａｌ．ＪＢｉｏｌｃｈｅｍ．１９９６；２７１(４)：１８２１－１８２４细胞内贮存钙的释放在一定程度上反应了１，４，５－三磷酸肌醇或咖啡因的?..

关键词：肌浆内质网量子释放酶的活性咖啡因嘌呤生物碱平滑肌

咖啡因引起平滑肌钙的量子释放依赖于肌浆内质网Ca2＋ATP酶的活性

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医学信息 1996年第8期 33-33页

作者：Ｊ．Ｍ．Ｓｔｅｎｂｅｒｇｅｎ,胡玉珍摘译

咖啡因引起平滑肌钙的量子释放依赖于肌浆内质网Ｃａ２＋ＡＴＰ酶的活性［英］Ｊ．Ｍ．Ｓｔｅｎｂｅｒｇｅｎ，ｅｔａｌ．ＪＢｉｏｌｃｈｅｍ．１９９６；２７１（４）：１８２１－１８２４细胞内贮存钙的释放在一定程度上反应了１，４... 详细信息

咖啡因引起平滑肌钙的量子释放依赖于肌浆内质网Ｃａ２＋ＡＴＰ酶的活性［英］Ｊ．Ｍ．Ｓｔｅｎｂｅｒｇｅｎ，ｅｔａｌ．ＪＢｉｏｌｃｈｅｍ．１９９６；２７１（４）：１８２１－１８２４细胞内贮存钙的释放在一定程度上反应了１，４，５—三磷酸肌醇或咖啡因的含量增加...

关键词：肌浆内质网量子释放咖啡因嘌呤生物碱 ATP Ca 平滑肌酶的活性

离体大鼠不均匀牵张膈肌标本上的神经肌肉传递

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中国应用生理学杂志 1994年第1期10卷 18-22页

作者：纪占欣刘传缋北京毒物药物研究所

在离体大鼠不均匀牵张膈肌标木（ＩＮＳＭＰ）上选择合适终板，可用同一微电极记录同一终板的小终板电住（ＭＥＰＰ）和终板电位（ＥＰＰ）。膜电位为－６２±２ｍＶ，ＭＥＰＰ频率和幅度在正常值范围，ＥＰＰ幅度高于２０ｍＶ，用直接... 详细信息

在离体大鼠不均匀牵张膈肌标木（ＩＮＳＭＰ）上选择合适终板，可用同一微电极记录同一终板的小终板电住（ＭＥＰＰ）和终板电位（ＥＰＰ）。膜电位为－６２±２ｍＶ，ＭＥＰＰ频率和幅度在正常值范围，ＥＰＰ幅度高于２０ｍＶ，用直接法计算的量子含量对细胞外Ｃａ２＋浓度双对数作图呈直线，斜率为４．０６。２、５０和１００Ｈ２串刺激诱发量子释放的统计学性质为二项分布。表明ＩＮＳＭＰ上合适终板可作为电生理学上接近正常生理状态的神经肌接头模型，并提示生理状态下神经肌接头突触前诱发量子释放的机率较高（５０Ｈｚ时ｐ＝０．９），遵循二项分布规律，递质动员较快（５０ＨＺ时为２０５０ｓ－１），以保证正常的神经肌肉传递。

关键词：神经肌肉传递终板电位量子释放

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巨蜥心脏内的血流

动物学报 1997年第S1期43卷 135-137页

巨蜥心脏内的血流ＢＬＯＯＤＣＩＲＣＵＬＡＴＩＯＮＩＮＴＨＥＨＥＡＲＴＯＦＭＯＮＩＴＯＲＬＩＺＡＲＤ（ＶＡＲＡＮＵＳＳＡＬＶＡＴＯＲ）关键词巨蜥心脏血液循环量子释放ＫｅｙｗｏｒｄｓＭｏｎｉｔｏｒｌｉｚａｒｄ，Ｈｅａｒｔ... 详细信息

巨蜥心脏内的血流ＢＬＯＯＤＣＩＲＣＵＬＡＴＩＯＮＩＮＴＨＥＨＥＡＲＴＯＦＭＯＮＩＴＯＲＬＩＺＡＲＤ（ＶＡＲＡＮＵＳＳＡＬＶＡＴＯＲ）关键词巨蜥心脏血液循环量子释放ＫｅｙｗｏｒｄｓＭｏｎｉｔｏｒｌｉｚａｒｄ，Ｈｅａｒｔｃｉｒｃｕｌａｔｉｏｎ，Ｑｕａｎｔ...

关键词：巨蜥心脏血液循环量子释放

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神经肌肉疾病

国外科技资料目录（医药卫生） 2002年第8期 194-195页

0231350 L5/S1神经根病时肌肉功能减弱与相关电生理参数变化/Scelsa S N∥Electromyogr Clin Neurophysiol.-2001,41(3).-145～151 冀医图0231351 与量子释放缺陷有关的突触前先天性肌无力综合征/Maselli R A∥Neurol-ogy.-2001,57(2).-... 详细信息

关键词：神经肌肉疾病先天性肌无力综合征肌肉功能神经根病量子释放电生理肌萎缩侧索硬化症突触前参数变化包涵体肌炎

静脉注射IgG的中和作用对Lambert-Eaton肌无力综合征患者的突触前效应

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世界核心医学期刊文摘（神经病学分册） 2005年第8期1卷 50-51页

作者： Buchwald B. Ahangari R. Weishaupt A. Toyka K.V. 李一明 Max Planck Institute of Psychiatry Neurology Section Neurophy siology Kraepelinstrasse 2- 10 80804 Munich Germany Dr.

Intravenous immunoglobulin (IVIg) treatment improves mus- cle strength in Lambert-Eaton myasthenic syndrome (LEMS), butits specific mod e of action is unknown. We have delineated its mode of action on neuromuscular b locking properties of LEMS IgG. The effect of sera and purified IgG from six pat ients with LEMS on evoked quantal release was investigated after direct applicat ion to the motor nerve terminal by the perfused macro-patch-clamp electrode in mouse hemidiaphragms. The effect of LEMS IgG was analyzed alone and after coinc ubation with different concentrations of IVIg or its Fab fragments. All LEMS ser a and purified LEMS IgG fractions taken before IVIg treatment inhibited evoked q uantal release in a dose-dependent manner. When LEMS IgG was coincubated with a therapeutic IVIg preparation, presynaptic inhibitory activity of LEMS IgG was d iminished in a dose-dependent fashion. Monovalent Fab fragments were as effecti ve in neutralizing the activity of LEMS IgG as whole IVIg. These direct neutrali zing effects of IVIg may explain its therapeutic efficacy.

关键词：肌无力 IgG Eaton 突触前抑制 Lambert 量子释放运动神经末梢鼠膈作用模式作用方式