检索结果-南通市图书馆

World Journal of Clinical Oncology 2025年第2期16卷 130-143页

作者： Wei-Wu Chen Yue-Hong Kong Li-Yuan Zhang Center for Cancer Diagnosis and Treatment The Second Affiliated Hospital of Soochow UniversitySuzhou 215000Jiangsu ProvinceChina

BACKGROUND Bone is a major site of metastasis in nasopharyngeal carcinoma(NPC).Recently,nuclear factor kappa-beta ligand(RANKL)inhibitors have garnered attention for their ability to inhibit osteoclast formation and bone resorption,as well as their potential to modulate immune functions and thereby enhance the efficacy of programmed cell death protein 1(PD-1)inhibitor *** SUMMARY We present a case of a patient with NPC who developed sternal stalk metastasis and multiple bone metastases with soft tissue invasion following radical chemoradiotherapy and targeted *** to chemotherapy,the patient experienced severe bone marrow suppression and opted out of further chemotherapy ***,the patient received combination therapy,including RANKL inhibitors(denosumab)alongside PD-1,radiotherapy,and granulocyte-macrophage colonystimulating factor(PRaG)therapy(NCT05435768),and achieved 16 months of progression-free survival and more than 35 months of overall survival,without encountering any grade 2 or higher treatment-related adverse *** Denosumab combined with PRaG therapy could be a new therapeutic approach for the second-line treatment in patients with bone metastases.

关键词： Nasopharyngeal carcinoma Bone metastasis Radiotherapy Programmed cell death protein 1 inhibitors Denosumab Case report

Albumin fusion with granulocyte-macrophage colony-stimulating factor acts as an immunotherapy against chronic tuberculosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2021年第10期18卷 2393-2401页

作者： Yu-Min Chuang Liangmei He Michael L.Pinn Ya-Chea Tsai Max A.Cheng Emily Farmer Petros C.Karakousis Chien-Fu Hung Department of Pathology Johns Hopkins University School of MedicineBaltimoreMDUSA Department of Medicine Johns Hopkins University School of MedicineBaltimoreMDUSA Section of Infectious Diseases Department of Internal MedicineYale University School of MedicineNew HavenCTUSA

A long duration of treatment and emerging drug resistance pose significant challenges for global tuberculosis(TB)eradication ***,there is an urgent need to develop novel strategies to shorten TB treatment regimens and to treat drug-resistant *** an albumin-fusion strategy,we created a novel albumin-fused granulocyte-macrophage colony-stimulating factor(albGM-CSF)molecule that harnesses albumin’s long half-life and targeting abilities to enhance the biostability of GM-CSF and direct it to the lymph nodes,where the effects of GM-CSF can increase dendritic cell populations crucial for eliciting a potent immune *** this study,we demonstrate that albGM-CSF serves as a novel immunotherapy for chronic Mycobacterium tuberculosis(Mtb)infections by enhancing GM-CSF biostability in ***,albumin is very safe,stable,and has a long half-life,thereby enhancing the biostability of *** the lungs and draining lymph nodes,albGM-CSF is able to increase the numbers of dendritic cells,which are crucial for the activation of naive T cells and for eliciting potent immune *** administration of albGM-CSF alone reduced the mean lung bacillary burden in mice with chronic tuberculosis *** GM-CSF administration was associated with IL-1βrelease from Mtb-infected dendritic cells and macrophages,higher IL-1βlevels were observed in albGM-CSF-treated mice with chronic tuberculosis infection than in mice receiving *** fusion with GM-CSF represents a promising strategy for the control of chronic lung tuberculosis infections and serves as a novel therapeutic vaccination platform for other infectious diseases and malignancies.

关键词： Tuberculosis albumin GM-CSF therapeutic vaccine fusion protein

Pharmacokinetics of recombinant human granulocyte macrophage colony-stimulating factor in Macaca mulata

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国药理学报 1998年第1期19卷 50-53页

作者：王仁刚朱兴族宋伟中国科学院上海药物研究所药理室

目的：研究重组人粒细胞巨噬细胞集落刺激因子（ｒｈＧＭＣＳＦ）在恒河猴体内的药物动力学．方法：用酶连接免疫吸附测定法检测血浆中ｒｈＧＭＣＳＦ的含量．结果：ｉｖｒｈＧＭＣＳＦ后血药浓度时间曲线符合三房室模型．第１，... 详细信息

目的：研究重组人粒细胞巨噬细胞集落刺激因子（ｒｈＧＭＣＳＦ）在恒河猴体内的药物动力学．方法：用酶连接免疫吸附测定法检测血浆中ｒｈＧＭＣＳＦ的含量．结果：ｉｖｒｈＧＭＣＳＦ后血药浓度时间曲线符合三房室模型．第１，２和３相的Ｔ１２分别为００５－００７ｈ，０１４－０５８ｈ和１４－４１ｈ．ＡＵＣ随剂量成比例增加．ｉｖ高剂量和低剂量的Ｃｌ和Ｋ１０都相似．ｓｃｒｈＧＭＣＳＦ后血药浓度的峰值为０９３±０１６μｇ·Ｌ－１，达峰时间为２６５±０１４ｈ，生物利用度为０６１．结论：恒河猴ｒｈＧＭＣＳＦ药物动力学数据为临床试验提供有用参考．

关键词：药物动力学 ELISA rhGM-CSF

维普期刊数据库

granulocyte-macrophage colony-stimulating factor protects mice against hepatocellular carcinoma by ameliorating intestinal dysbiosis and attenuating inflammation

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2020年第36期26卷 5420-5436页

作者： Yong-Na Wu Lei Zhang Tuo Chen Xun Li Li-Hong He Guang-Xiu Liu Key Laboratory of Desert and Desertification Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou 730000Gansu ProvinceChina Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province Lanzhou 730000Gansu ProvinceChina University of Chinese Academy of Sciences Beijing 100049China The First Hospital of Lanzhou University Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Key Laboratory of Biological Therapy and Regenerative Medicine Transformation Gansu Province Lanzhou 730000Gansu ProvinceChina

BACKGROUND Hepatocellular carcinoma(HCC)is the third leading cause of cancer mortality *** gut microbiota can help maintain healthy metabolism and ***-macrophage colony-stimulating factor(GM-CSF)is a critical factor in promoting health and homeostasis;it promotes intestinal immunity,stimulates bone marrow precursors to generate macrophage colonies,and enhances the antibacterial and antitumor activity of circulating *** such,GM-CSF may protect against HCC development by regulating immunity as well as intestinal *** To investigate the impact of GM-CSF on the gut microbiome and metabolic characteristics of *** Thirty-six male BALB/c nude mice were divided into three groups:Control(n=10),HCC(n=13),and HCC+GM-CSF(GM-CSF overexpression,n=13).We utilized HCC cells to establish orthotopic transplantation tumor models of HCC with normal and over-expressing *** injury,immune inflammatory function and intestinal barrier function were *** fecal microbiome and metabolome were studied using 16S rRNA absolute quantification sequencing and gas chromatography-mass *** GM-CSF overexpression significantly affected the gut microbiome of mice with HCC and resulted in a high abundance of organisms of the genera Roseburia,Blautia and Butyricimonass,along with a significant reduction in Prevotella,Parabacteroides,Anaerotruncus,Streptococcus,Clostridium,and ***,GM-CSF overexpression resulted in a substantial increase in fecal biotin and oleic acid levels,along with a prominent decrease in the fecal succinic acid,adenosine,fumaric acid,lipoic acid,and maleic acid *** analysis revealed that the intestinal microbiota and fecal metabolites induced by GM-CSF were primarily involved in pathways related to reducing the inflammatory response,biotin metabolism,and intestinal barrier *** GM-CSF can protect against HCC development by regulating immunity and

关键词： granulocyte-macrophage colony-stimulating factor Microbiome Inflammation Hepatocellular carcinoma

Preclinical evaluation of herpes simplex virus armed with granulocyte-macrophage colony-stimulating factor in pancreatic carcinoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2013年第31期19卷 5138-5143页

作者： Hao Liu Shou-Jun Yuan Ying-Tai Chen Yi-Bin Xie Liang Cui Wei-Zhi Yang De-Xuan Yang Yan-Tao Tian Department of Abdominal Surgery Cancer Hospital Chinese Academy of Medical Sciences Peking Union Medical College Pharmacological and Toxi-cological Laboratory Institute of Radiation Medicine Medical Radiobiology Laboratory Cancer Hospital Chinese Academy of Medical Science Peking Union Medical College

AIM: To investigate the therapeutic efficacy and mechanisms of action of oncolytic-herpes-simplex-virus encoding granulocyte-macrophage colony-stimulating factor(HSVGM-CSF) in pancreatic ***: Tumor blocks were homogenized in a sterile grinder in *** homogenate was injected into the right armpit of each *** vaccination,the mice were randomly assigned into four groups: a control group,a high dose HSVGM-CSFgroup [1 × 107plaque forming units(pfu)/tumor],a medium dose HSVGM-CSF group(5 × 106pfu/tumor) and a low dose HSVGM-CSF group(5 × 105pfu/tumor).After initiation of drug administration,body weights and tumor diameters were measured every 3 *** days later,after decapitation of the animal by cervical dislocation,each tumor was isolated,weighed and stored in 10% formaldehyde *** drug effectiveness was evaluated according to the weight,volume and relative volume change of each ***,GM-CSF protein levels in serum were assayed by enzyme-linked immunosorbent assays at 1,2,3 and 4 d after injection of ***: Injection of the recombinant mouse HSV encoding GM-CSF resulted in a significant reduction in tumor growth compared to the control group,and dosedependent effects were observed: the relative tumor proliferation rates of the low dose,medium dose and high dose groups on 15 d after injection were 45.5%,55.2% and 65.5%,*** inhibition rates of the tumor weights of the low,middle,and high dose groups were 41.4%,46.7% and 50.5%,***,the production of GM-CSF was significantly increased in the mice infected with *** increase in the GM-CSF level was more pronounced in the high dose group compared to the other two dose ***: Our study provides the first evidence that HSVGM-CSFcould inhibit the growth of pancreatic *** enhanced GM-CSF expression might be responsible for the phenomenon.

关键词： Pancreatic carcinoma Gene therapy Animal test Herpes-simplex-virus encoding granulocytemacrophage colony-stimulating factor

The clinical effectiveness and safety of using epidermal growth factor,fibroblast growth factor and granulocyte-macrophage colony stimulating factor as therapeutics in acute skin wound healing:a systematic review and meta-analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Burns & Trauma 2022年第1期10卷 715-741页

作者： Yating Wei Jiangfeng Li Yao Huang Xun Lei Lijun Zhang Meifang Yin Jiawen Deng Xiaoyan Wang Xiaobing Fu Jun Wu Department of Burn and Plastic Surgery Department ofWound RepairShenzhen Institute of Translational Medicinethe First Affiliated Hospital of Shenzhen UniversityShenzhen Second People’s HospitalShenzhenChina Shenzhen University ShenzhenChina School of Public Health and Management Chongqing Medical UniversityChongqingChina The First Affiliated Hospital Sun Yat-sen UniversityGuangzhouChina Research Center for Wound Repair and Tissue Regeneration Medical Innovation Research DepartmentThe Key Laboratory of PLAWound Repair and Tissue Regenerationthe Fourth Medical Center of PLA General Hospitalthe PLA General HospitalBeijing 100048China

Background:Promoting wound healing is crucial to restore the vital barrier function of injured *** factor products including epidermal growth factor(EGF),fibroblast growth factor(FGF)and granulocyte-macrophage colony stimulating factor(GM-CSF)have been used for decades although no systematic evaluation exists regarding their effectiveness and safety issues in treating acute skin *** has resulted in a lack of guidelines and standards for proper application ***,this systematic review and meta-analysis was performed to critically evaluate the effectiveness and safety of these growth factors on skin acute wounds and provide guidelines for application ***:We searched PubMed/Medline(1980-2020),Cochrane Library(1980-2020),Cochrane CENTRAL(from establishment to 2020),***(from establishment to 2020),Chinese Journal Full-text Database(CNKI,1994-2020),China Biology Medicine disc(CBM,1978-2019),Chinese Scientific Journal Database(VIP,1989-2020)andWanfang Database(WFDATA,1980-2019).Randomized controlled trials(RCTs),quasi-RCTs and controlled clinical trials treating patients with acute skin wounds from various causes and with those available growth factors were ***:A total of 7573 papers were identified through database searching;229 papers including 281 studies were kept after final *** growth factors significantly short-ened the healing time of acute skin wounds,including superficial burn injuries[mean differ-ence(MD)=−3.02;95%confidence interval(CI):−3.31∼−2.74;pfactors increased the healing rate of acute skin wounds and decreased scar *** incidence of adverse reactions was lower in the growth factor treatment group than in the non-growth factor ***:The studied growth factors not only are effective and safe for managing acute sk

关键词： Growth factors Skin wounds Meta-analysis Wound healing

Impaired granulocyte-macrophage colony-stimulating factor bioactivity accelerates surgical recurrence in ileal crohn's disease

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2018年第5期24卷 623-630页

作者： Grace Gathungu Yuanhao Zhang Xinyu Tian Erin Bonkowski Leahana Rowehl Julia Krumsiek Billy Nix Claudia Chalk Bruce Trapnell Wei Zhu Rodney Newberry Lee Denson Ellen Li Department of Pediatrics Division of Pediatric Gastroenterology Stony Brook University Department of Medicine Division of Gastroenterology Stony Brook University Department of Pediatrics Cincinnati Children’s Hospital Medical Center Department of Medicine Washington University St.Louis Applied Mathematics and Statistics Stony Brook University

AIM To examine the relationship between elevated granulocyte-macrophage colony-stimulating factor(GMCSF) auto-antibodies(Ab) level and time to surgical recurrence after initial surgery for Crohn's disease(CD). METHODS We reviewed 412 charts from a clinical database at tertiary academic hospital. Patients included in the study had ileal or ileocolonic CD and surgical resection of small bowel or ileocecal region for management of disease. Serum samples were analyzed for serological assays including GM-CSF cytokine, GM-CSF Ab, ASCA Ig G and Ig A, and genetic markers including SNPs rs2066843, rs2066844, rs2066845, rs2076756 and rs2066847 in NOD2, rs2241880 in ATG16 L1, and rs13361189 in IRGM. Cox proportional-hazards models were used to assess the predictors of surgical *** Ninety six percent of patients underwent initial ileocecal resection(ICR) or ileal resection(IR) and subsequently 40% of patients required a second ICR/IR for CD. GMCSF Ab level was elevated at a median of 3.81 mcg/mL. factors predicting faster time to a second surgery included elevated GM-CSF Ab [hazard ratio(HR) 3.52, 95%CI: 1.45-8.53, P = 0.005] and elevated GM-CSF cytokine(HR = 2.48, 95%CI: 1.31-4.70, P = 0.005). factors predicting longer duration between first and second surgery included use of Immunomodulators(HR = 0.49, 95%CI: 0.31-0.77, P = 0.002), the interaction effect of low GM-CSF Ab levels and smoking(HR = 0.60, 95%CI: 0.45-0.81, P = 0.001) and the interaction effect of low GM-CSF cytokine levels and ATG16 L1(HR = 0.65, 95%CI: 0.49-0.88, P = 0.006).CONCLUSION GM-CSF bioavailability plays a critical role in maintaining intestinal homeostasis. Decreased bioavailability coupled with the genetic risk markers and/or smoking results in aggressive CD behavior.

关键词： Inflammatory bowel disease granulocytemacrophage colony-stimulating factor antibody Crohn’s disease surgery

CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTOR WITH granulocyte-macrophage colony-stimulating factor GENE

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Cancer Research 2000年第2期12卷 125-127页

作者：郑秋红郑天荣谢云青卢林陈晖 Department of Tumor Molecular Biology Fujian Tumor Hospital Fuzhou China

Objective: To construct the eukaryotic expression vector that express human granulocyte-macrophage colony-stimulating factor (hGM-CSF) gene for making highly express in mammalian cells. Methods: Extract totally RNA from the induced human fetal lung (HFL) cell line. HGM-CSF cDNA was obtained by reverse transcription-polymerase chain reaction (RT-PCR), and then directionally subcloned into the HindIII and EcoRI site on the pcDNA3.1 plasmid, which was controlled by the CMV promoter, to form the recombinant expressing vector pcDNA3.1-GM-CSF. Results: The PCR amplification was identified and the sequence was analyzed, the results showed that hGM-CSF was properly inserted into the vector and the sequence was correct.

关键词： Human granulocyte-macrophage colony-stimulating factor (hGM-CSF) Reverse transcription and polymerse chain reaction (RT-PCR) Eukaryotic expression

维普期刊数据库

Effects of granulocyte-macrophage colony stimulating factor on the repair of vessel intima damaged by balloon

在线全文

学校读者我要写书评

暂无评论

中华医学杂志(英文版) 2005年第3期000卷 45-50页

作者： ZHANG Xing-hua, MA Xiao-jing and ZHAO Tong Department of Cardiology, Shandong Provincial Hospital, Jinan 250021, ChinaDepartment of Intensive Care Unit, Shandong Provincial Hospital, Jinan 250021, ChinaDepartment of Cardiology, Second Affiliated Hospital of Shandong University, Jinan 250000, China Department of Cardiology Shandong Provincial Hospital Jinan 250021 China Department of Intensive Care Unit Shandong Provincial Hospital Jinan 250021 China Department of Cardiology Second Affiliated Hospital of Shandong University Jinan 250000 China

Background The dysfunction of vascular endothelial cells plays a key role in starting and facilitating restenosis. The acceleration of intima repair and the recovery of endothelial function would reduce the restenosis rate. This study was undertaken to assess the effect of granulocyte-macrophage colony stimulating factor (GM-CSF) on the repair of damaged iliac arteries.

关键词： granulocyte-macrophage colony stimulating factor · angioplasty · hyperplasia

ANTITUMOR EFFECT OF granulocyte-macrophage colony-stimulating factor(GM-CSF)-GENE ENCODED VACCINIA MELANOMA ONCOLYSATE AND ITS IMMUNOLOGICAL MECHANISMS

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Cancer Research 1997年第4期9卷 31-35页

作者：鞠佃文曹雪涛万涛章卫平陶群于益芝陈国友

Vaccinia melanoma oncolysate (VMO) prepared by infecting B16F10 melanoma cells with recombinant vaccinia virus encoding murine GMCSF gene was tested for its therapeutic effect on the preestablished melanoma. C57BL/6 mice were inoculated s.c. with 1×105 B16F10 melanoma cells and received s.c. administration with VMO prepared with GMCSF gene encoded vaccinia virus(GMCSFVMO), VMO prepared with thymidine kinase genedeficient vaccinia virus(TKVMO), B16F10 melanoma oncolysate(BMO), or PBS 3 days after tumor inoculation. The same treatment was bolstered one week later. The results demonstrated that GMCSFVMO treatment significantly inhibited the growth of subcutaneous tumor and prolonged the survival period of tumorbearing mice. Further study elucidated that cytotoxicity of PBL and splenocytes towards B16F10 increased obviously after treatment with GMCSFVMO, but NK activity remained unchanged. These results suggest that the tumor oncolysate vaccine prepared with GMCSF geneencoded vaccinia virus might exert potent therapeutic effect on the preestablished tumor through the efficient induction of specific antitumor immune response of the host.

关键词： Vaccinia virus Gene therapy Melanoma,granulocytemacrophage colonystimulating factor Oncolysate Antitumor immunity

维普期刊数据库